| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
The aim of this research project is to develop a method to determine the binding sites of specific transcription factors that play important roles in specific cell lineages, such as primordial germ cells. To purify genomic DNA bound to a specific transcription factor, I developed knock-in mice that carry tags in the particular transcription factor. Furthermore, I developed a method to quantitatively amplify genomic DNA bound to a transcription factor. By these methods, it has become plausible to determine genomic sites bound to a transcription factor in a small cell number, such as 10, 000 cells.
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