Development of an efficient chromatin pull-down method from small cell numbers
| Project/Area Number |
21770251
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto University (2010-2011)
The Institute of Physical and Chemical Research (2009) |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 生殖細胞 / エピジェネティクス / ChIP / 次世代シークエンサー / 転写因子 / Blimp1 / Oct3/4 / 始原生殖細胞 / 転写制御因子 / 免疫沈降 / Oct3 / 4 |
|---|
| Research Abstract |
The aim of this research project is to develop a method to determine the binding sites of specific transcription factors that play important roles in specific cell lineages, such as primordial germ cells. To purify genomic DNA bound to a specific transcription factor, I developed knock-in mice that carry tags in the particular transcription factor. Furthermore, I developed a method to quantitatively amplify genomic DNA bound to a transcription factor. By these methods, it has become plausible to determine genomic sites bound to a transcription factor in a small cell number, such as 10, 000 cells.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] TDRD5 is required for retrotransposon silencing, chromatoid body assembly and spermiogenesis in mice2011
Author(s)
Yabuta, Y., Ohta, H., Abe, T., Kurimoro, K., Chuma, S., and Saitou, M
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Journal Title
The Journal of Cell Biology
Volume: 192
Issue: 5
Pages: 781-795
DOI
Related Report
Peer Reviewed
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[Journal Article] TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice2011
Author(s)
Yabuta, Y Ohta, H.Abe, T., Kurimoto, K.Chuma, S, Saitou, M.
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Journal Title
J Cell Biol
Volume: 192
Pages: 781-95
Related Report
Peer Reviewed
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[Journal Article] Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells2009
Author(s)
Yamaguchi, S., Kurimoto, K., Yabuta, Y., Sasaki, H., Nakatsuji, N., Saitou, M. & Tada, T
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Journal Title
Development
Volume: 136
Issue: 23
Pages: 4011-4020
DOI
Related Report
Peer Reviewed
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