| Project/Area Number |
21770253
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設) (2011)
National Institutes of Natural Sciences Okazaki Research Facilities (2009-2010) |
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Principal Investigator |
HAYASHI Yoshiki 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 助教 (30508817)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 生殖幹細胞 / ニッチ / ショウジョウバエ / プロテオグリカン / HSPG / グリピカン / Dally / Dally-like / dally-like |
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| Research Abstract |
Stem cell posses the remarkable characteristics to produce its daughter cell that retain a stem-cell identity and the other that differentiates. Stem cells reside in dedicated cellular microenvironments termed stem-cell niches. These niches dictate a stem-cell identity, maintain the stem cell population, and coordinate proper homeostatic production of differentiated cells. Drosophila germ line stem cells(GSCs) are one of the ideal models for stem cell study in vivo. In apical tips of both male and female gonads, GSCs associate with specially differentiated somatic gonadal cells, the niche cells. These niche cells generate niches for GSCs by secreting signaling molecules. Although these facts indicate that region of niche is defined by the spatial distribution of these ligands, molecular mechanisms that regulate ligand distribution within the niche is not characterized.
In this study, we showed that Drosophila Heparan Sulfate Proteoglycans(HSPGs) are essential components of GSC niche. Drosophila two glypians, a subfamily of HSPGs, were expressed in niche cells of female and male gonads and were required for GSC maintenance. Conversely, when glypican was ectopically expressed in ovaries, we observed expansion of GSC niche. These results showed that these glypicans define niche region molecularly.
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