Experimental verification of the requirement of micro-environment for continuous self-replication system.

• Project/Area Number: 21770256
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Evolutionary biology
• Research Institution: Osaka University
• Principal Investigator: ICHIHASHI Norikazu Osaka University, 大学院・情報科学研究科, 招聘教員 (20448096)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 細胞サイズ / エマルション / 自己複製 / RNA複製 / 寄生体 / 無細胞翻訳系 / RNA / マイクロ流路 / 複製酵素

Research Abstract

Most of the self-sustained cell are micro-meter size ; there are no larger cells or smaller cells. In order to investigate the importance of micro-size of cells, I constructed an artificial self-replication system of genetic information, and examine the size-dependency of it. I found that the replication proceeded continuously only in micro-size reaction volume because of the repression of the propagation of parasitic replicator. This result suggested that the cell size is strongly connected with defense mechanism against parasite.

Research Products

• [Journal Article] Identification of two forms of Qβ replicase with different thermal stabilities but identical RNA replication activity (2010)
  • Author(s): Norikazu Ichihashi, Tomoaki Matsuura, Kazufumi Hosoda, Tetsuya Yomo
  • Journal Title: The Journal of Biological Chemistry 285 Pages: 37210-37217
  • Peer Reviewed
• [Journal Article] Compartmentalization in a water-in-oil emulsion repressed the spontaneous amplification of RNA by Qβ replicase (2010)
  • Author(s): Hiroya Urabe, Norikazu Ichihashi, Tomoaki Matsuura, Kazufumi Hosoda, Yasuaki. Kazuta, Hiroshi Kita, Tetsuya Yomo
  • Journal Title: Biochemistry 49 Pages: 1809-1813
  • Peer Reviewed
• [Journal Article] Identification of two forms of Qβrephcase with different thermal stabilities but identical RNA replication activity (2010)
  • Author(s): 市橋伯一
  • Journal Title: The Journal of Biological Chemistry Volume: 285 Pages: 37210-37217
  • Peer Reviewed
• [Presentation] Constructing an artificial self-replication system of genetic information from RNA and proteins (2010)
  • Author(s): 市橋伯一
  • Organizer: 12th International Conference on the Synthesis and Simulation of Living Systems (Alife XII)
  • Place of Presentation: Odense, Denmark
  • Year and Date: 2010-08-22
• [Presentation] Constructing self-replication system in liposome. (2009)
  • Author(s): 市橋伯一
  • Organizer: International Symposium on Complex System Biology
  • Place of Presentation: 東京
  • Year and Date: 2009-09-29
• [Book] The Origins of Life (2010)
  • Author(s): Norikazu Ichihashi, Tomoaki Matsuura, Hiroshi Kita, Takeshi Sunami, Hiroaki Suzuki, Tetsuya Yomo
  • Publisher: COLD SPRING HARBOR LABORATORY PRESS
• [Book] The Origins of Life (2010)
  • Author(s): 市橋伯一
  • Publisher: COLD SPRING HARBOR LABORATORY PRESS
• [Remarks] ホームページ等
  • URL: http://www-symbio.ist.osaka-u.ac.jp/
• [Remarks]
  • URL: http://www-symbio.ist.oska-u.ac.jp/
• [Patent(Industrial Property Rights)] エマルジョンを用いたRNAの選択的増幅法 (2009)
  • Inventor(s): 四方哲也、松浦友亮、市橋伯一、占部博也
  • Industrial Property Rights Holder: 科学技術振興機構
  • Industrial Property Number: 2009-168285
  • Filing Date: 2009-07-16