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Identification of novel regulatory of flowering time using multiple mutants of rice(Oryza sativa L.)

Research Project

Project/Area Number 21780002
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionKyoto University

Principal Investigator

SAITO Hiroki  京都大学, 大学院・農学研究科, 助教 (10536238)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords育種学 / 遺伝学 / 植物 / 発生・分化 / 出穂期 / 突然変異系統 / イネ
Research Abstract

To identify novel genes controlling flowering time in rice, flowering time mutants were investigated. High resolution molecular marker analyses showed that an early flowering time mutant, HS112, harbored a mutation in the gene(Os03g0151300) in chromosome 3, and another early flowering time mutant, HS254, harbored a mutant gene in chromosome 10. It is not reported that these genes involve in controlling flowering time. Therefore, it is considered that these genes are novel flowering time genes, called Se14 and Se15. Expression analyses demonstrated that a flowering time gene, Ehd1, was highly up-regulated under long-day condition in both of these mutants. These results indicate that Se14 and Se15 repress the expression of Ehd1 under long-day condition. Multiple mutants based on known mutant lines, X61, HS169 and double-mutant line of these two lines, were obtained. In total 15, 000 lines, 168 plants were selected as flowering time mutants. These novel mutants will provide us new knowledge to understand how to control flowering time in rice.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (12 results)

All 2012 2011 2009

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (9 results)

  • [Journal Article] Ef7 Encodes an ELF3-like Protein and Promotes Rice Flowering by Negatively Regulating the Floral Repressor Gene Ghd7 under Both Short- and Long-Day Conditions2012

    • Author(s)
      Saito H, Ogiso-Tanaka E, Okumoto Y, Yoshitake Y, Izumi H, Yokoo T, Matsubara K, Hori K, Yano M, Inoue H, Tanisaka T
    • Journal Title

      Plant and cell physiology

      Volume: 53 Issue: 4 Pages: 717-728

    • DOI

      10.1093/pcp/pcs029

    • NAID

      10030653553

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complete loss of photoperiodic response in the rice mutant line X61 is caused by deficiency of phytochrome chromophore biosynthesis gene2011

    • Author(s)
      Saito H, Okumoto Y, Yoshitake Y, Inoue H, Yuan Q, Teraishi M, Tsukiyama T, Nishida H, Tanisaka T
    • Journal Title

      Theor. Appl. Genet

      Volume: 122巻 Issue: 1 Pages: 109-118

    • DOI

      10.1007/s00122-010-1426-2

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Complete loss of photoperiodic response in the rice mutant line X61 is caused by deficiency of phytochrome chromophore biosynthesis gene2011

    • Author(s)
      Saito H, Okumoto Y, Yoshitake Y, Inouo H, Yuan Q, Teraishi M, Tsukiyama T, Nishida H, Tanisaka T
    • Journal Title

      Theoretical and Applied Genetics

      Volume: 122 Pages: 109-118

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] イネ早生突然変異系統の超長日条件における出穂遅延特性の多様性2011

    • Author(s)
      齊藤大樹、横尾敬行、徐銓、吉竹良洋、浅野翔、泉はるか、Kamal Mostafa、山川真梨恵、奥本裕
    • Organizer
      日本育種学会
    • Place of Presentation
      横浜市立大学
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Ehd1の発現制御に関わる新規感光性遺伝子の単離2011

    • Author(s)
      横尾敬行、齊藤大樹、浅見武人、徐銓、吉竹良洋、浅野翔、泉はるか、伊藤真、K. Mustafa、谷坂隆俊、奥本裕
    • Organizer
      日本育種学会第120回講演会
    • Place of Presentation
      宇都宮大学
    • Related Report
      2011 Final Research Report
  • [Presentation] イネ早生突然変異系統の超長日条件における出穂遅延特性の多様性2011

    • Author(s)
      齊藤大樹、横尾敬行、徐銓、吉竹良洋、浅野翔、泉はるか、Kamal Mostafa、山川真梨恵、奥本裕
    • Organizer
      日本育種学会第119回講演会
    • Place of Presentation
      横浜市立大学
    • Related Report
      2011 Final Research Report
  • [Presentation] 不感光性突然変異系統X61のもつ新規突然変異遺伝子の単離およびその育種的利用2009

    • Author(s)
      齊藤大樹・吉竹良洋・奥本裕・谷坂隆俊
    • Organizer
      日本育種学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 極早生突然変異系統X61のもつ不感光性遺伝子se13の機能解析2009

    • Author(s)
      吉竹良洋・齊藤大樹・浅見武人・浅野翔・築山拓司・河内孝之・三瀬和之・奥本裕・谷坂隆俊
    • Organizer
      日本育種学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] mPing SCARマーカーを新規出穂期突然変異遺伝子の同定2009

    • Author(s)
      浅見武人・齊藤大樹・徐銓・奥本裕・袁清波・吉竹良洋・谷坂隆俊
    • Organizer
      日本育種学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] mPing SCARマーカーを用いた新規出穂期突然変異遺伝子の同定2009

    • Author(s)
      浅見武人、齊藤大樹、徐銓、奥本裕、袁清波、吉竹良洋、谷坂隆俊
    • Organizer
      日本育種学会第116回講演会
    • Place of Presentation
      北海道大学
    • Related Report
      2011 Final Research Report
  • [Presentation] 不感光性突然変異系統X61のもつ新規突然変異遺伝子の単離およびその育種的利用2009

    • Author(s)
      齊藤大樹、吉竹良洋、袁清波、奥本裕、谷坂隆俊
    • Organizer
      日本育種学会第116回講演会
    • Place of Presentation
      北海道大学
    • Related Report
      2011 Final Research Report
  • [Presentation] 極早生突然変異系統X61のもつ不感光性遺伝子se13の機能解析2009

    • Author(s)
      吉竹良洋、齊藤大樹、浅見武人、浅野翔、築山卓司、河内孝之、三瀬和之、奥本裕、谷坂隆俊
    • Organizer
      日本育種学会第116回講演会
    • Place of Presentation
      北海道大学
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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