| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
Gene targeting(GT) via homologous recombination is a powerful transformation technology because it enables to modify the endogenous target gene as expected. In this study, we succeeded in the production of novel tryptophan(Trp)-fortified rice plants by GT-mediated targeted mutagenesis of OASA2 gene that encodes a key enzyme in Trp biosynthesis of rice. In the GT plant, other amino acids except for Trp and the metabolites synthesized from Trp such as serotonin, vitamin B and indole alkaloid accumulated at a higher level also. Thus, we demonstrated that GT-mediated targeted mutagenesis is applicable to metabolic engineering in crops.
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