Molecular breeding of tryptophan-fortified rice via gene targeting
| Project/Area Number |
21780010
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
SAIKA Hiroaki 独立行政法人農業生物資源研究所, ゲノム機能改変研究ユニット, 主任研究員 (20435613)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 遺伝子ターゲッティング / 点変異 / 代謝改変 / トリプトファン / 相同組換え / 遺伝子組換え植物 / アミノ酸アナログ |
|---|
| Research Abstract |
Gene targeting(GT) via homologous recombination is a powerful transformation technology because it enables to modify the endogenous target gene as expected. In this study, we succeeded in the production of novel tryptophan(Trp)-fortified rice plants by GT-mediated targeted mutagenesis of OASA2 gene that encodes a key enzyme in Trp biosynthesis of rice. In the GT plant, other amino acids except for Trp and the metabolites synthesized from Trp such as serotonin, vitamin B and indole alkaloid accumulated at a higher level also. Thus, we demonstrated that GT-mediated targeted mutagenesis is applicable to metabolic engineering in crops.
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Report
(4 results)
Research Products
(43 results)
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[Presentation] 新規シトクロムP450遺伝子CYP72A31はイネにおける除草剤ビスピリバックナトリウム耐性に関与する2012
Author(s)
雑賀啓明, 田口(塩原)文緒, 堀清純, 田中剛, 松本隆, 伊藤剛, 矢野昌裕, 堀田順子, 角康一郎, 清水力, 土岐精一
Organizer
日本育種学会第121回講演会
Place of Presentation
宇都宮大学
Year and Date
2012-03-30
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