| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
The glycosyltransferase(GT) and acyltransferase(AT) genes involved in anthocyanin biosynthesis were cloned and transformed into chrysanthemums to development of techniques for the gene regulation. We cloned genes that probably encode acyl-CoA : anthocyanin 3-or 5-acyltransferase from Lobelia. Perilla anthocyanin 3AT was suitable for aromatic acylation to the glucose of anthocyanin position 3 in chrysanthemum flowers. The petals of transgenic chrysanthemums that introduced petal specific promoter driven and translational enhancer fused Clitoria 3' 5' GT were synthesized and accumulated B-ring glycosylated anthocyanins. We also generated transgenic chrysanthemum plants co-expressing genes for B-ring hydroxylation, glycosylation and acylation. In the near future, we will characterize these transgenic chrysanthemum lines.
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