Study of anthocyanin modification genes that cause flower color diversity and development of techniques for the gene regulation
| Project/Area Number |
21780033
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
NODA Naonobu 独立行政法人農業・食品産業技術総合研究機構, 花き研究所花き研究領域, 主任研究員 (10455313)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 花色 / 遺伝子組換え / アントシアニン / 糖転移酵素 / アシル基転移酵素 |
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| Research Abstract |
The glycosyltransferase(GT) and acyltransferase(AT) genes involved in anthocyanin biosynthesis were cloned and transformed into chrysanthemums to development of techniques for the gene regulation. We cloned genes that probably encode acyl-CoA : anthocyanin 3-or 5-acyltransferase from Lobelia. Perilla anthocyanin 3AT was suitable for aromatic acylation to the glucose of anthocyanin position 3 in chrysanthemum flowers. The petals of transgenic chrysanthemums that introduced petal specific promoter driven and translational enhancer fused Clitoria 3' 5' GT were synthesized and accumulated B-ring glycosylated anthocyanins. We also generated transgenic chrysanthemum plants co-expressing genes for B-ring hydroxylation, glycosylation and acylation. In the near future, we will characterize these transgenic chrysanthemum lines.
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Report
(4 results)
Research Products
(7 results)
