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Analysis on the cell-to-cell movement mechanism mediated by cross-talk of a plant virus and NCAPs

Research Project

Project/Area Number 21780035
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant pathology
Research InstitutionThe University of Tokyo

Principal Investigator

YAMAJI Yasuyuki  The University of Tokyo, 大学院・農学生命科学研究科, 助教 (40345187)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords病害抵抗性 / NCAP / TMV / クロストーク
Research Abstract

In the researches of plant viruses, the molecular machinery of the cell-to-cell movement process is poorly understood. In this study, we focused plant non-cell autonomous proteins (NCAPs) and analyzed the cross-talks between the cell-to cell movement of NCAPs and tobacco mosaic virus (TMV). We showed that a tobacco NTH2O1, a KN1 family protein, and a tobacco NtMPIP1, a heat-shock protein, cooperatively promote the cell-to-cell movement of TMV via the interaction with TMV MP. These results elucidated a part of the cell-to-cell movement mechanism and established the basis of anti-viral strategy to inhibit the cell-to-cell movement.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (31 results)

All 2010 2009

All Journal Article (20 results) (of which Peer Reviewed: 20 results) Presentation (11 results)

  • [Journal Article] Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection.2010

    • Author(s)
      Yamaji, Y., Sakurai, K., Hamada, K., Komatsu, K., Ozeki, J., Yoshida, A., Yoshii, A., Shimizu, T., Namba, S., Hibi, T.
    • Journal Title

      Arch Virol. 155

      Pages: 263-268

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory effect on the tobacco mosaic virus infection by a plant RING finger protein.2010

    • Author(s)
      Yamaji, Y., Hamada, K., Yoshinuma, T., Sakurai, K., Yoshii, A., Shimizu, T., Hashimoto, M., Suzuki, M., Namba, S., Hibi, T.
    • Journal Title

      Virus Res. 152

      Pages: 50-57

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Viral-induced systemic necrosis in plants involves both programmed cell death and the inhibition of viral multiplication, which are regulated by independent pathways.2010

    • Author(s)
      Komatsu, K., Hashimoto, M., Ozeki, J., Yamaji, Y., Maejima, K., Senshu, H., Himeno, M., Okano, Y., Kagiwada, S., Namba, S.
    • Journal Title

      Mol.Plant-Microbe Interact. 23

      Pages: 283-293

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic heterogeneity found in the replicase gene of poinsettia mosaic virus isolates.2010

    • Author(s)
      Okano, Y., Maejima, K., Shiraishi, T., Hashimoto, M., Senshu, H., Ozeki, J., Takahashi, S., Komatsu, K., Yamaji, Y., Namba, S.
    • Journal Title

      Arch Virol. 155

      Pages: 1367-1370

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pseudo-polyprotein translated from the full-length ORF1 of capillovirus is important for pathogenicity, but a truncated ORF1 protein without variable and CP regions is sufficient for replication.2010

    • Author(s)
      Hirata, H., Yamaji, Y., Komatsu, K., Kagiwada, S., Oshima, K., Okano, Y., Takahashi, S., Ugaki, M., Namba, S.
    • Journal Title

      Virus Res. 152

      Pages: 1-9

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] First report of plum pox virus infecting Japanese apricot (Prunus mume Sieb. et Zucc.) in Japan.2010

    • Author(s)
      Maejima, K., Hoshi, H., Hashimoto, M., Himeno, M., Kawanishi, T., Komatsu, K., Yamaji, Y., Hamamoto, H., Namba, S.
    • Journal Title

      J.Gen.Plant Pathol. 76

      Pages: 229-231

    • NAID

      10029719709

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection.2010

    • Author(s)
      Yamaji, Y., Sakurai, K., Hamada, K., Komatsu, K., Ozeki, J., Yoshida, A., Yoshii, A., Shimizu, T., Namba, S., Hibi
    • Journal Title

      Arch Virol

      Volume: 155 Pages: 263-268

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory effect on the tobacco mosaic virus infection by a plant RING finger protein.2010

    • Author(s)
      Yamaji, Y., Hamada, K., Yoshinuma, T., Sakurai, K., Yoshii, A., Shimizu, T., Hashimoto, M., Suzuki, M., Namba S.
    • Journal Title

      Virus Res.

      Volume: 152 Pages: 50-57

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Viral-induced systemic necrosis in plants involves both programmed cell death and the inhibition of viral multiplication, which are regulated by independent pathways.2010

    • Author(s)
      Komatsu, K., Hashimoto, M., Ozeki, J., Yamaji, Y., Maejima, K., Senshu, H., Himeno, M., Okano, Y., Kagiwada, S.
    • Journal Title

      Mol.Plant-Microbe Interact

      Volume: 23 Pages: 283-293

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic heterogeneity found in the replicase gene of poinsettia mosaic virus isolates.2010

    • Author(s)
      Okano, Y., Maejima, K., Shiraishi, T., Hashimoto, M., Senshu, H., Ozeki, J., Takahashi, S., Komatsu, K., Yamaii, Y.
    • Journal Title

      Arch.Virol.

      Volume: 155 Pages: 1367-1370

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pseudo-polyprotein translated from the full-length ORF1 of capillovirus is important for pathogenicity, but a truncated ORF1 protein without variable and CP regions is sufficient for replication2010

    • Author(s)
      Hirata, H., Yamaji, Y., Komatsu, K., Kagiwada, S., Oshima, K., Okano, Y., Takahashi, S., Ugaki, M., Namba, S.
    • Journal Title

      Virus Res.

      Volume: 152 Pages: 1-9

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] First report of plum pox virus infecting Japanese apricot (Prunus mume Sieb.et Zucc.) in Japan.2010

    • Author(s)
      Maejima, K., Hoshi, H., Hashimoto, M., Himeno, M., Kawanishi, T., Komatsu, K., Yamaii, Y., Hamamoto, H., Namba, S.
    • Journal Title

      J.Gen.Plant Pathol

      Volume: 76 Pages: 229-231

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Significantly low level of small RNA accumulation derived from an encapsidated mycovirus with dsRNA genome2010

    • Author(s)
      Himeno M, Maejima K, Komatsu K, Ozeki J, Hashimoto M, Kagiwada S, Yamaji Y, Namba S
    • Journal Title

      Virology 396

      Pages: 69-75

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The N-terminal region of the Plantago asiatica mosaic virus coat protein is required for cell-to-cell movement, but is dispensable for virion assembly.2009

    • Author(s)
      Ozeki J, Hashimoto M, Komatsu K, Maejima K, Himeno M, Senshu H, Kagiwada T, Yamaji Y, Namba S.
    • Journal Title

      Mol.Plant-Microbe Interact. 22

      Pages: 677-685

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a novel tobacco DnaJ-like protein that interacts with the movement protein of tobacco mosaic virus.2009

    • Author(s)
      Shimizu T, Yoshii A, Sakurai K, Hamada K, Yamaji Y, Suzuki M, Namba S, Hibi T.
    • Journal Title

      Arch Virol. 154

      Pages: 959-967

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Complete nucleotide sequence and genome organization of butterbur mosaic virus.2009

    • Author(s)
      Hashimoto M, Komatsu K, Maejima K, Yamaji Y, Okano Y, Shiraishi T, Takahashi S, Kagiwada S, Namba S.
    • Journal Title

      Arch Virol. 154

      Pages: 1955-1958

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Significantly low level of small RNA accumulation derived from an encapsidated mycovirus with dsRNA genome.2009

    • Author(s)
      Himeno M, Maejima K, Komatsu K, Ozeki J, Hashimoto M, Kagiwada S, Yamaji Y, Namba S.
    • Journal Title

      Virology. 396

      Pages: 69-75

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The N-terminal region of the Plantago asiatica mosaic virus coat protein is required for cell-to-cell movement, but is dispensable for virion assembly2009

    • Author(s)
      Ozeki J, Hashimoto M, Komatsu K, Maejima K, Himeno M, Senshu H, Kawanishi T, Kagiwada S, Yamaji Y, Namba S.
    • Journal Title

      Mol.Plant-Microb.Interact. 22

      Pages: 677-685

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a novel tobacco DnaJ-like protein that interacts with the movement protein of tobacco mosaic virus2009

    • Author(s)
      Shimizu T, Yoshii A, Sakurai K, Hamada K, Yamaji Y, Suzuki M, Namba S, Hibi T.
    • Journal Title

      Arch Virol 154

      Pages: 959-967

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complete nucleotide sequence and genome organization of butterbur mosaic virus2009

    • Author(s)
      Hashimoto M, Komatsu K, Maejima K, Yamaji Y, Okano Y, Shiraishi T, Takahashi S, Kagiwada S, Namba S
    • Journal Title

      Arch Virol 154

      Pages: 1955-1958

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] タバコモザイクウイルスの感染における翻訳伸長因子eEF1Aの機能解析2010

    • Author(s)
      山次康幸・桜井慶太郎・小松健・難波成任・日比忠明
    • Place of Presentation
      国立京都国際会館(京都府)
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Role of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection2010

    • Author(s)
      Yamaji Y, Okano Y, Shiraish Ti, Komatsu K, Hashimoto M, Namba S, Hibi T
    • Organizer
      2010 European molecular biology organization workshop
    • Place of Presentation
      トリノ(イタリア)
    • Related Report
      2010 Final Research Report
  • [Presentation] Viral-Induced Systemic Necrosis in Plants Involves Both Programmed Cell Death and the Inhibition of Viral Multiplication, Which are Regulated by Independent Pathways2010

    • Author(s)
      Hashimoto M, Komatsu K, Ozeki J, Yamaji Y, Maejima K, Senshu H, Himeno M, Okano Y, Shiraishi T, Namba S
    • Organizer
      2010 European molecular biology organization workshop
    • Place of Presentation
      トリノ(イタリア)
    • Related Report
      2010 Final Research Report
  • [Presentation] Role of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection.2010

    • Author(s)
      Yamaji Y, Okano Y, Shiraish Ti, Komatsu K, Hashimoto M, Namba S, Hibi T
    • Organizer
      2010 European molecular biology organization workshop
    • Place of Presentation
      Torino, Italy
    • Related Report
      2010 Annual Research Report
  • [Presentation] Viral-Induced Systemic Necrosis in Plants Involves Both Programmed Cell Death and the Inhibition of Viral Multiplication, Which are Regulated by Independent Pathways2010

    • Author(s)
      Hashimoto M, Komatsu K, Ozeki J, Yamaji Y, Maejima K, Senshu H, Himeno M, Okano Y, Shiraishi T.
    • Organizer
      2010 European molecular biology organization workshop
    • Place of Presentation
      Torino, Italy
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物ウイルスのRNAサイレンシング抑制タンパク質の抑制能の比較解析2009

    • Author(s)
      千秋博子・尾関丈二・小松健・橋本将典・青山倫子・畑田康司・山次康幸・難波成任
    • Organizer
      平成21年度日本分子生物学会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] Potexvirus属ウイルスの細胞間移行には外被タンパク質の3番目のロイシン残基が必須である2009

    • Author(s)
      尾関丈二、橋本将典、青山倫子、小松健、川西剛史、岡野夕香里、山次康幸、難波成任
    • Organizer
      平成21年度日本分子生物学会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] わが国のウメにおいて初めて感染が確認されたplum pox virus(PPV)(ウメ輪紋ウイルス:和名新称)について2009

    • Author(s)
      萱野佑典・小野剛・前島健作・星秀男・川西剛史・山次康幸・橋本光司・濱本宏・難波成任
    • Organizer
      平成21年度日本植物病理学会関東部会
    • Place of Presentation
      藤沢
    • Related Report
      2010 Final Research Report
  • [Presentation] イムノクロマト法によるウメ輪紋ウイルス(plum pox virus, PPV)の簡易迅速高感度検出キットの開発2009

    • Author(s)
      前島健作・萱野佑典・姫野未紗子・小松健・白石拓也・山次康幸・北村暢夫・大上光明・濱本宏・難波成任
    • Organizer
      平成21年度日本植物病理学会関東部会
    • Place of Presentation
      藤沢
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] Potexvirus 属ウイルスの細胞間移行には外被タンパク質の3番目のロイシン残基が必須である2009

    • Author(s)
      尾関丈二、橋本将典、青山倫子、小松健、川西剛史、岡野夕香里、山次康幸、難波成任
    • Organizer
      平成21年度日本分子生物学会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] わが国のウメにおいて初めて感染が確認された plum pox virus(PPV)(ウメ輪紋ウイルス:和名新称)について2009

    • Author(s)
      萱野佑典・小野剛・前島健作・星秀男・川西剛史・山次康幸・橋本光司・濱本宏・難波成任
    • Organizer
      平成21年度日本植物病理学会関東部会
    • Place of Presentation
      藤沢
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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