Analysis on the cell-to-cell movement mechanism mediated by cross-talk of a plant virus and NCAPs

• Project/Area Number: 21780035
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: The University of Tokyo
• Principal Investigator: YAMAJI Yasuyuki The University of Tokyo, 大学院・農学生命科学研究科, 助教 (40345187)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 病害抵抗性 / NCAP / TMV / クロストーク

Research Abstract

In the researches of plant viruses, the molecular machinery of the cell-to-cell movement process is poorly understood. In this study, we focused plant non-cell autonomous proteins (NCAPs) and analyzed the cross-talks between the cell-to cell movement of NCAPs and tobacco mosaic virus (TMV). We showed that a tobacco NTH2O1, a KN1 family protein, and a tobacco NtMPIP1, a heat-shock protein, cooperatively promote the cell-to-cell movement of TMV via the interaction with TMV MP. These results elucidated a part of the cell-to-cell movement mechanism and established the basis of anti-viral strategy to inhibit the cell-to-cell movement.

Research Products

• [Journal Article] Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection. (2010)
  • Author(s): Yamaji, Y., Sakurai, K., Hamada, K., Komatsu, K., Ozeki, J., Yoshida, A., Yoshii, A., Shimizu, T., Namba, S., Hibi, T.
  • Journal Title: Arch Virol. 155 Pages: 263-268
  • Peer Reviewed
• [Journal Article] Inhibitory effect on the tobacco mosaic virus infection by a plant RING finger protein. (2010)
  • Author(s): Yamaji, Y., Hamada, K., Yoshinuma, T., Sakurai, K., Yoshii, A., Shimizu, T., Hashimoto, M., Suzuki, M., Namba, S., Hibi, T.
  • Journal Title: Virus Res. 152 Pages: 50-57
  • Peer Reviewed
• [Journal Article] Viral-induced systemic necrosis in plants involves both programmed cell death and the inhibition of viral multiplication, which are regulated by independent pathways. (2010)
  • Author(s): Komatsu, K., Hashimoto, M., Ozeki, J., Yamaji, Y., Maejima, K., Senshu, H., Himeno, M., Okano, Y., Kagiwada, S., Namba, S.
  • Journal Title: Mol.Plant-Microbe Interact. 23 Pages: 283-293
  • Peer Reviewed
• [Journal Article] Genetic heterogeneity found in the replicase gene of poinsettia mosaic virus isolates. (2010)
  • Author(s): Okano, Y., Maejima, K., Shiraishi, T., Hashimoto, M., Senshu, H., Ozeki, J., Takahashi, S., Komatsu, K., Yamaji, Y., Namba, S.
  • Journal Title: Arch Virol. 155 Pages: 1367-1370
  • Peer Reviewed
• [Journal Article] Pseudo-polyprotein translated from the full-length ORF1 of capillovirus is important for pathogenicity, but a truncated ORF1 protein without variable and CP regions is sufficient for replication. (2010)
  • Author(s): Hirata, H., Yamaji, Y., Komatsu, K., Kagiwada, S., Oshima, K., Okano, Y., Takahashi, S., Ugaki, M., Namba, S.
  • Journal Title: Virus Res. 152 Pages: 1-9
  • Peer Reviewed
• [Journal Article] First report of plum pox virus infecting Japanese apricot (Prunus mume Sieb. et Zucc.) in Japan. (2010)
  • Author(s): Maejima, K., Hoshi, H., Hashimoto, M., Himeno, M., Kawanishi, T., Komatsu, K., Yamaji, Y., Hamamoto, H., Namba, S.
  • Journal Title: J.Gen.Plant Pathol. 76 Pages: 229-231
  • NAID: 10029719709
  • Peer Reviewed
• [Journal Article] Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection. (2010)
  • Author(s): Yamaji, Y., Sakurai, K., Hamada, K., Komatsu, K., Ozeki, J., Yoshida, A., Yoshii, A., Shimizu, T., Namba, S., Hibi
  • Journal Title: Arch Virol Volume: 155 Pages: 263-268
  • Peer Reviewed
• [Journal Article] Inhibitory effect on the tobacco mosaic virus infection by a plant RING finger protein. (2010)
  • Author(s): Yamaji, Y., Hamada, K., Yoshinuma, T., Sakurai, K., Yoshii, A., Shimizu, T., Hashimoto, M., Suzuki, M., Namba S.
  • Journal Title: Virus Res. Volume: 152 Pages: 50-57
  • Peer Reviewed
• [Journal Article] Viral-induced systemic necrosis in plants involves both programmed cell death and the inhibition of viral multiplication, which are regulated by independent pathways. (2010)
  • Author(s): Komatsu, K., Hashimoto, M., Ozeki, J., Yamaji, Y., Maejima, K., Senshu, H., Himeno, M., Okano, Y., Kagiwada, S.
  • Journal Title: Mol.Plant-Microbe Interact Volume: 23 Pages: 283-293
  • Peer Reviewed
• [Journal Article] Genetic heterogeneity found in the replicase gene of poinsettia mosaic virus isolates. (2010)
  • Author(s): Okano, Y., Maejima, K., Shiraishi, T., Hashimoto, M., Senshu, H., Ozeki, J., Takahashi, S., Komatsu, K., Yamaii, Y.
  • Journal Title: Arch.Virol. Volume: 155 Pages: 1367-1370
  • Peer Reviewed
• [Journal Article] Pseudo-polyprotein translated from the full-length ORF1 of capillovirus is important for pathogenicity, but a truncated ORF1 protein without variable and CP regions is sufficient for replication (2010)
  • Author(s): Hirata, H., Yamaji, Y., Komatsu, K., Kagiwada, S., Oshima, K., Okano, Y., Takahashi, S., Ugaki, M., Namba, S.
  • Journal Title: Virus Res. Volume: 152 Pages: 1-9
  • Peer Reviewed
• [Journal Article] First report of plum pox virus infecting Japanese apricot (Prunus mume Sieb.et Zucc.) in Japan. (2010)
  • Author(s): Maejima, K., Hoshi, H., Hashimoto, M., Himeno, M., Kawanishi, T., Komatsu, K., Yamaii, Y., Hamamoto, H., Namba, S.
  • Journal Title: J.Gen.Plant Pathol Volume: 76 Pages: 229-231
  • Peer Reviewed
• [Journal Article] Significantly low level of small RNA accumulation derived from an encapsidated mycovirus with dsRNA genome (2010)
  • Author(s): Himeno M, Maejima K, Komatsu K, Ozeki J, Hashimoto M, Kagiwada S, Yamaji Y, Namba S
  • Journal Title: Virology 396 Pages: 69-75
  • Peer Reviewed
• [Journal Article] The N-terminal region of the Plantago asiatica mosaic virus coat protein is required for cell-to-cell movement, but is dispensable for virion assembly. (2009)
  • Author(s): Ozeki J, Hashimoto M, Komatsu K, Maejima K, Himeno M, Senshu H, Kagiwada T, Yamaji Y, Namba S.
  • Journal Title: Mol.Plant-Microbe Interact. 22 Pages: 677-685
  • Peer Reviewed
• [Journal Article] Identification of a novel tobacco DnaJ-like protein that interacts with the movement protein of tobacco mosaic virus. (2009)
  • Author(s): Shimizu T, Yoshii A, Sakurai K, Hamada K, Yamaji Y, Suzuki M, Namba S, Hibi T.
  • Journal Title: Arch Virol. 154 Pages: 959-967
  • Peer Reviewed
• [Journal Article] Complete nucleotide sequence and genome organization of butterbur mosaic virus. (2009)
  • Author(s): Hashimoto M, Komatsu K, Maejima K, Yamaji Y, Okano Y, Shiraishi T, Takahashi S, Kagiwada S, Namba S.
  • Journal Title: Arch Virol. 154 Pages: 1955-1958
  • Peer Reviewed
• [Journal Article] Significantly low level of small RNA accumulation derived from an encapsidated mycovirus with dsRNA genome. (2009)
  • Author(s): Himeno M, Maejima K, Komatsu K, Ozeki J, Hashimoto M, Kagiwada S, Yamaji Y, Namba S.
  • Journal Title: Virology. 396 Pages: 69-75
  • Peer Reviewed
• [Journal Article] The N-terminal region of the Plantago asiatica mosaic virus coat protein is required for cell-to-cell movement, but is dispensable for virion assembly (2009)
  • Author(s): Ozeki J, Hashimoto M, Komatsu K, Maejima K, Himeno M, Senshu H, Kawanishi T, Kagiwada S, Yamaji Y, Namba S.
  • Journal Title: Mol.Plant-Microb.Interact. 22 Pages: 677-685
  • Peer Reviewed
• [Journal Article] Identification of a novel tobacco DnaJ-like protein that interacts with the movement protein of tobacco mosaic virus (2009)
  • Author(s): Shimizu T, Yoshii A, Sakurai K, Hamada K, Yamaji Y, Suzuki M, Namba S, Hibi T.
  • Journal Title: Arch Virol 154 Pages: 959-967
  • Peer Reviewed
• [Journal Article] Complete nucleotide sequence and genome organization of butterbur mosaic virus (2009)
  • Author(s): Hashimoto M, Komatsu K, Maejima K, Yamaji Y, Okano Y, Shiraishi T, Takahashi S, Kagiwada S, Namba S
  • Journal Title: Arch Virol 154 Pages: 1955-1958
  • Peer Reviewed
• [Presentation] タバコモザイクウイルスの感染における翻訳伸長因子eEF1Aの機能解析 (2010)
  • Author(s): 山次康幸・桜井慶太郎・小松健・難波成任・日比忠明
  • Place of Presentation: 国立京都国際会館(京都府)
• [Presentation] Role of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection (2010)
  • Author(s): Yamaji Y, Okano Y, Shiraish Ti, Komatsu K, Hashimoto M, Namba S, Hibi T
  • Organizer: 2010 European molecular biology organization workshop
  • Place of Presentation: トリノ(イタリア)
• [Presentation] Viral-Induced Systemic Necrosis in Plants Involves Both Programmed Cell Death and the Inhibition of Viral Multiplication, Which are Regulated by Independent Pathways (2010)
  • Author(s): Hashimoto M, Komatsu K, Ozeki J, Yamaji Y, Maejima K, Senshu H, Himeno M, Okano Y, Shiraishi T, Namba S
  • Organizer: 2010 European molecular biology organization workshop
  • Place of Presentation: トリノ(イタリア)
• [Presentation] Role of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection. (2010)
  • Author(s): Yamaji Y, Okano Y, Shiraish Ti, Komatsu K, Hashimoto M, Namba S, Hibi T
  • Organizer: 2010 European molecular biology organization workshop
  • Place of Presentation: Torino, Italy
• [Presentation] Viral-Induced Systemic Necrosis in Plants Involves Both Programmed Cell Death and the Inhibition of Viral Multiplication, Which are Regulated by Independent Pathways (2010)
  • Author(s): Hashimoto M, Komatsu K, Ozeki J, Yamaji Y, Maejima K, Senshu H, Himeno M, Okano Y, Shiraishi T.
  • Organizer: 2010 European molecular biology organization workshop
  • Place of Presentation: Torino, Italy
• [Presentation] 植物ウイルスのRNAサイレンシング抑制タンパク質の抑制能の比較解析 (2009)
  • Author(s): 千秋博子・尾関丈二・小松健・橋本将典・青山倫子・畑田康司・山次康幸・難波成任
  • Organizer: 平成21年度日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Potexvirus属ウイルスの細胞間移行には外被タンパク質の3番目のロイシン残基が必須である (2009)
  • Author(s): 尾関丈二、橋本将典、青山倫子、小松健、川西剛史、岡野夕香里、山次康幸、難波成任
  • Organizer: 平成21年度日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] わが国のウメにおいて初めて感染が確認されたplum pox virus(PPV)(ウメ輪紋ウイルス:和名新称)について (2009)
  • Author(s): 萱野佑典・小野剛・前島健作・星秀男・川西剛史・山次康幸・橋本光司・濱本宏・難波成任
  • Organizer: 平成21年度日本植物病理学会関東部会
  • Place of Presentation: 藤沢
• [Presentation] イムノクロマト法によるウメ輪紋ウイルス(plum pox virus, PPV)の簡易迅速高感度検出キットの開発 (2009)
  • Author(s): 前島健作・萱野佑典・姫野未紗子・小松健・白石拓也・山次康幸・北村暢夫・大上光明・濱本宏・難波成任
  • Organizer: 平成21年度日本植物病理学会関東部会
  • Place of Presentation: 藤沢
• [Presentation] Potexvirus 属ウイルスの細胞間移行には外被タンパク質の3番目のロイシン残基が必須である (2009)
  • Author(s): 尾関丈二、橋本将典、青山倫子、小松健、川西剛史、岡野夕香里、山次康幸、難波成任
  • Organizer: 平成21年度日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] わが国のウメにおいて初めて感染が確認された plum pox virus(PPV)(ウメ輪紋ウイルス:和名新称)について (2009)
  • Author(s): 萱野佑典・小野剛・前島健作・星秀男・川西剛史・山次康幸・橋本光司・濱本宏・難波成任
  • Organizer: 平成21年度日本植物病理学会関東部会
  • Place of Presentation: 藤沢