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Proteome analysis of plant innate immunity mediated by OsRac1 complex

Research Project

Project/Area Number 21780036
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant pathology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

FUJIWARA Masayuki  奈良先端科学技術大学院大学, バイオサイエンス研究科, 特任助教 (70403350)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords植物・病原体相互作用 / プロテオーム / シグナル伝達 / 植物自然免疫
Research Abstract

It has previously been shown that OsRac1 is involved in defense response for infecting of rice blast fungus. In this work, I analyzed OsRac1 by using biochemical techniques. The analysis of gel filtration indicated that OsRac1 formed complex(es) at very high molecular range. Furthermore, it was shown that this complex contained OsRac1 was changed molecular size by stimulus of pathogen.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (40 results)

All 2012 2011 2010 2009 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (32 results) Remarks (4 results)

  • [Journal Article] The Hop/Sti1-Hsp90 chaperone complex facilitates the maturation and transport of a PAMP receptor in rice innate immunity2010

    • Author(s)
      Chen, L., Hamada, S., Fujiwara, M., Zhu, T., Thao, N.P., Wong, H.L., Krishna, P., Ueda, T., Kaku, H., Shibuya, N., Kawasaki, T., and Shimamoto, K.
    • Journal Title

      Cell Host Microbe

      Volume: 7 Pages: 185-196

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Hop/Stil-Hsp90 chaperone complex facilitates the maturation and transport of a PAMP receptor in rice innate immunity2010

    • Author(s)
      Letian Chen
    • Journal Title

      Cell Host & Microbe

      Volume: 7 Pages: 185-196

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteome analysis of detergent-resistant membranes (DRMs) associated with OsRac1-mediated innate immunity in rice2009

    • Author(s)
      Fujiwara, M., Hamada, S., Hiratsuka, M., Fukao, Y., Kawasaki, T., and Shimamoto, K.
    • Journal Title

      Plant Cell Physiol.

      Volume: 50 Pages: 1191-1200

    • NAID

      10027341177

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteome analysis of detergent-resistant membranes (DRMs) associated with OsRacl-mediated innate immunity in rice.2009

    • Author(s)
      Masayuki Fujiwara
    • Journal Title

      Plant Cell Physiology 50

      Pages: 1191-1200

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] イネの自然免疫におけるDefensome 複合体の解析2012

    • Author(s)
      濱田聡, 藤原正幸, 島本功
    • Organizer
      平成24年度植物病理学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2012-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] イネの自然免疫におけるDefensome複合体の解析2012

    • Author(s)
      藤原正幸
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      福岡県福岡市
    • Year and Date
      2012-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of the Defensome complex in rice innate immunity2012

    • Author(s)
      Satoshi Hamada, Masayuki Fujiwara, Ko Shimamoto
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-17
    • Related Report
      2010 Final Research Report
  • [Presentation] Defensome は免疫受容体を含む2011

    • Author(s)
      濱田聡, 藤原正幸, 島本功
    • Organizer
      平成23年度植物病理学会大会
    • Place of Presentation
      高松
    • Year and Date
      2011-10-02
    • Related Report
      2010 Final Research Report
  • [Presentation] Defensomeは免疫受容体を含む2011

    • Author(s)
      濱田聡
    • Organizer
      日本植物病理学会関西部会
    • Place of Presentation
      香川県高松市
    • Year and Date
      2011-10-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物細胞膜マイクロドメインのプロテオーム解析2011

    • Author(s)
      藤原正幸, 濱田聡, 深尾陽一朗, 島本功
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-17
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物細胞膜マイクロドメインのプロテオーム解析2011

    • Author(s)
      藤原正幸
    • Organizer
      日本植物学会
    • Place of Presentation
      東京都目黒区(招待講演)
    • Year and Date
      2011-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 低分子量Gタンパク質OsRac1 が制御するイネ免疫機構のプロテオーム解析2011

    • Author(s)
      藤原正幸, 濱田聡, 島本功
    • Organizer
      日本プロテオーム学会2011年大会
    • Place of Presentation
      新潟
    • Year and Date
      2011-07-29
    • Related Report
      2010 Final Research Report
  • [Presentation] 低分子量Gタンパク質OsRac1が制御するイネ免疫機構のプロテオーム解析2011

    • Author(s)
      藤原正幸
    • Organizer
      日本プロテオーム学会
    • Place of Presentation
      新潟県新潟市(招待講演)
    • Year and Date
      2011-07-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物免疫を制御するOsRac1 複合体 Defensome の解析2011

    • Author(s)
      濱田聡, 藤原正幸, 島本功
    • Organizer
      平成23年度植物病理学会大会
    • Place of Presentation
      府中
    • Year and Date
      2011-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物免疫を制御するOsRac1複合体Defensomeの解析2011

    • Author(s)
      濱田聡
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      東京都府中市
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of the Defensome complex in rice innate immunity2011

    • Author(s)
      Satoshi Hamada
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      京都府京都市
    • Year and Date
      2011-03-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of the Defensome complex in rice innate immunity2011

    • Author(s)
      Satoshi Hamada, Masayuki Fujiwara, Ko Shimamoto
    • Organizer
      The 2^<nd> ICBPI
    • Place of Presentation
      Kunming China
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of the Defensome complex in rice innate immunity2011

    • Author(s)
      Satoshi Hamada
    • Organizer
      The 2^<nd> ICBPI
    • Place of Presentation
      China Kunming(ポスター発表複数日)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of the Defensome complex in rice innate immunity2010

    • Author(s)
      Satoshi Hamada, Masayuki Fujiwara, Ko Shimamoto
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of the Defensome complex in rice innate immunity2010

    • Author(s)
      Satoshi Hamada
    • Organizer
      BMB2010
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 活性型OsRac1 は高分子量複合体(Defensome)を形成する2010

    • Author(s)
      濱田 聡, 藤原正幸, 島本功
    • Organizer
      平成22年度植物病理学会大会
    • Place of Presentation
      京都
    • Year and Date
      2010-04-19
    • Related Report
      2010 Final Research Report
  • [Presentation] イネ免疫応答におけるOsRac1(低分子量G タンパク質)複合体の解析2010

    • Author(s)
      藤原正幸, 濱田聡, 深尾陽一朗, 川崎努, 島本功
    • Organizer
      平成22年度植物病理学会大会
    • Place of Presentation
      京都
    • Year and Date
      2010-04-19
    • Related Report
      2010 Final Research Report
  • [Presentation] 活性型OsRac1は高分子量複合体(Defensome)を形成する2010

    • Author(s)
      濱田聡
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      京都府京都市
    • Year and Date
      2010-04-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物免疫を制御するOsRac1 複合体Defensome の解析2010

    • Author(s)
      濱田聡, 藤原正幸, 島本功
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] イネ免疫機構を制御するタンパク質複合体Defensome のプロテオーム解析2010

    • Author(s)
      藤原正幸, 濱田聡, 深尾陽一朗, 川崎努, 島本功
    • Organizer
      第51 回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物免疫を制御するOsRacl複合体Defensomeの解析2010

    • Author(s)
      濱田聡
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      熊本県 熊本市
    • Year and Date
      2010-03-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] イネ免疫機構を制御するタンパク質複合体Defensomeのプロテオーム解析2010

    • Author(s)
      藤原正幸
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      熊本県 熊本市
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] Role of lipid raft domain in OsRac1-mediated rice innate immunity2009

    • Author(s)
      Masayuki Fujiwara, Satoshi Hamada, Minori Hiratsuka, Yoichiro Fukao, Tsutomu Kawasaki, Ko Shimamoto
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物免疫を制御するOsRacl複合体(Defensome)の解析2009

    • Author(s)
      藤原正幸
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      神奈川県 横浜市
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物免疫を制御するOsRac1 複合体(Defensome)の解析2009

    • Author(s)
      濱田聡, 藤原正幸, 島本功
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物免疫を制御するOsRacl複合体(Defensome)の解析2009

    • Author(s)
      濱田聡
    • Organizer
      日本生化学会大会
    • Place of Presentation
      兵庫県 神戸市
    • Year and Date
      2009-10-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Proteome analysis of lipid rafts associated with rice innate immunity mediated by OsRac12009

    • Author(s)
      Masayuki Fujiwara, Satoshi Hamada, Yoichiro Fukao, Tsutomu Kawasaki, Ko Shimamoto
    • Organizer
      14th IS-MPMI
    • Place of Presentation
      Quebec City, Canada
    • Year and Date
      2009-07-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of the OsRac1 complex (Defensome) involved in rice innate immunity2009

    • Author(s)
      Satoshi Hamada, Masayuki Fujiwara, Ko Shimamoto
    • Organizer
      14^<th> IS-MPMI
    • Place of Presentation
      Quebec City, Canada
    • Year and Date
      2009-07-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Proteome analysis of lipid rafts associated with rice innate immunity mediated by OsRacl2009

    • Author(s)
      Masayuki Fujiwara
    • Organizer
      IS-MPMI
    • Place of Presentation
      カナダ ケベック
    • Year and Date
      2009-07-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物免疫のプロテオミクス2009

    • Author(s)
      島本功, 藤原正幸, 濱田聡, 深尾陽一朗, 川崎努
    • Organizer
      第61回日本細胞生物学会大会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-06-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物免疫のプロテオミクス2009

    • Author(s)
      藤原正幸
    • Organizer
      日本細胞生物学会大会
    • Place of Presentation
      愛知県 名古屋市
    • Year and Date
      2009-06-04
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://bsw3.naist.jp/plantglobal/team/protein_ntwk/index.shtml

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://bsw3.aist-nara.ac.jp/simamoto/research_sick.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://bsw3.naist.jp/plantglobal/team/protein_ntwk/index.shtml

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://bsw3.aist-nara.ac.jp/simamoto/research_sick.html

    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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