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molecular biological approach for Fe-deficiency symptom of plants at high Ni soil and suppression method

Research Project

Project/Area Number 21780054
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant nutrition/Soil science
Research InstitutionMie University

Principal Investigator

MIZUNO Takafumi  三重大学, 大学院・生物資源学研究科, 准教授 (50346003)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords高ニッケル土壌 / シロイヌナズナ / IRT1 / 鉄輸送体 / 酵母 / 蛇紋岩 / ニッケル / 鉄 / 競合吸収
Research Abstract

In hydroponic cultures, excess Ni exposure increased Fe accumulation and the relative transcription level of AtIRT1 in roots, indicating that excess Ni induces AtIRT1 expression in roots. Moreover, Ni accumulation under Fe-deficient conditions was markedly lower in AtIRT1-defective mutants than in the wild-type, Col-0. These data demonstrate that AtIRT1 transports Ni^<2+> in roots, and strongly suggest that Ni accumulation is further accelerated by AtIRT1 that is expressed in response to excess Ni. We also investigated metal contents and their correlation of the flora of the serpentine soil area in Suga Island, Toba in Mie prefecture. Among the elements investigated, Fe concentration showed high correlation with Ni in these plants.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (40 results)

All 2012 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (23 results) Book (4 results) Remarks (3 results)

  • [Journal Article] Induction of IRT1 by the nickel-induced iron-deficient response in Arabidopsis2012

    • Author(s)
      Nishida S, Aisu A, and Mizuno T
    • Journal Title

      Plant Signaling and Behavior

      Volume: 7 Pages: 1-3

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] AtIRT1, the Primary Iron Uptake Transporter in the Root, Mediates Excess Nickel Accumulation in Arabidopsis thaliana2011

    • Author(s)
      Nishida S, Tsuzuki C, Kato A, Aisu A, Yoshida J and Mizuno T
    • Journal Title

      Plant Cell Physiology

      Volume: 52 Pages: 1433-1442

    • NAID

      10029713668

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the N-terminal Region of TjZNT2, a Zrt/Irt-like Protein Family Metal Transporter, as a Novel Functional Region Involved in Metal Ion Selectivity2011

    • Author(s)
      Nishida S, Morinaga Y, Obata H and Mizuno T
    • Journal Title

      FEBS Journal

      Volume: 278 Pages: 851-858

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] AtIRT1, the Primary Iron Uptake Transporter in the Root, Mediates Excess Nickel Accumulation in Arabidopsis thaliana2011

    • Author(s)
      Nishida S, Tsuzuki C, Kato A, Aisu A, Yoshida J, Mizuno T
    • Journal Title

      Plant Cell Physiol

      Volume: 52 Pages: 1433-1442

    • NAID

      10029713668

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assessment of willow (Salix sp.) as a woody heavy metal accumulator : field survey and in vivo X-ray analyses2011

    • Author(s)
      Harada E, Hokura A, Nakai I, Terada Y, Baba K, Yazaki K, Shiono M, Mizuno N, Mizuno T
    • Journal Title

      Metallomics

      Volume: 3 Pages: 1340-1346

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the N-terminal Region of TjZNT2, a Zrt/Irt-like Protein Family Metal Transporter, as a NovelFunctional Region Involved in Metal Ion Selectivity.2011

    • Author(s)
      NIshida S, Morinaga Y, Obata H, Mizuno T
    • Journal Title

      FEBS Journal

      Volume: 278 Pages: 851-858

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterisation of foliar Mn in Mn-(hyper)accumulators using X-ray absorption spectroscopy2010

    • Author(s)
      Fernando DR, Mizuno T, Woodrow IE, Baker AJM, Collins RN
    • Journal Title

      New Phytologist

      Volume: 188 Pages: 1014-1027

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of multidrug resistance associated protein like gene from lead hyperaccumulator common buckwheat and its lead detoxification ability.2010

    • Author(s)
      Mizuno T, Nakagawa M, Ono H, Sugiura D, Tamura H, Obata H.
    • Journal Title

      Plant Biotechnology (In press)

    • NAID

      10026411374

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Serpentine plants in Hokkaido and their chemicalcharacteristics2009

    • Author(s)
      Mizuno T, Horie K, Nosaka S, Obata H, and Mizuno N
    • Journal Title

      Northeastern Naturalist

      Volume: 16 Pages: 65-80

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serpentine plants in Hokkaido and their chemical characteristics.2009

    • Author(s)
      Mizuno T, Horie K, Nosaka S, Obata H, Mizuno N.
    • Journal Title

      Northeaster Naturalist. 16

      Pages: 65-80

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Mineral properties of the plants grown on serpentine soil of Suga Island2012

    • Author(s)
      Yoko Kirihata, Takafumi Mizuno
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Nickel uptake via Zrt/Irt-like Protein (ZIP) family transporters in Arabidopsis2012

    • Author(s)
      Sho Nishida, Aki Kato, Chisato Tsuzuki, Ayaka Aisu, Kyohei Murakami, Takafumi Mizuno
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヤナギ(Salix sp.)の重金属集積に関する研究:野外調査および放射光蛍光X線分析法2011

    • Author(s)
      原田英美子、保倉明子、中井泉、寺田靖子、馬場啓一、矢崎一史、水野直治、水野隆文
    • Organizer
      第29回日本植物細胞分子生物学会大会
    • Place of Presentation
      九州大学(博多)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] The potential of the manganese (Mn) hyperaccumulator Chengiopanax sciadophylloides for phytoremediation and phytomining2011

    • Author(s)
      Takafumi Mizuno
    • Organizer
      11th International Conference on the Biogeochemistry of Trace Elements (ICOBTE)
    • Place of Presentation
      Florence, Italy(招待講演)
    • Year and Date
      2011-07-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] Assessment of heavy metal accumulation in a woody metal accumulator willow (Salix sp.) : a field survey and in vivo X-ray analyses2011

    • Author(s)
      Harada E, Hokura A, Nakai I, Terada Y, Baba K, Yazaki K, Mizuno N, Mizuno T
    • Organizer
      3rd International Symposiumu on Metallomics
    • Place of Presentation
      Munster, Germany
    • Year and Date
      2011-06-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物のニッケル耐性におけるZIPトランスポーターの役割2011

    • Author(s)
      西田翔, 愛須彩加, 水野隆文
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台(東北大学)
    • Year and Date
      2011-03-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 重金属集積植物Thlaspi japonicum由来重金属トランスポーターの機能解析2011

    • Author(s)
      西田翔・愛須彩加・水野隆文
    • Organizer
      日本土壌肥料学会2011大会
    • Place of Presentation
      つくば国際会議場(つくば)
    • Related Report
      2011 Final Research Report
  • [Presentation] マンガン超集積植物コシアブラからのマンガン資源回収に関する研究2010

    • Author(s)
      水野隆文・江守香苗・橋本洋平
    • Organizer
      土壌肥料学会2010年度大会
    • Place of Presentation
      札幌(北海道大学)
    • Year and Date
      2010-09-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物の亜鉛吸収経路を介したニッケルの過剰吸収2010

    • Author(s)
      西田翔・加藤亜季・都築千里・愛須彩加・鰐部順平・小畑仁・水野隆文
    • Organizer
      土壌肥料学会2010年度大会
    • Place of Presentation
      札幌(北海道大学)
    • Year and Date
      2010-09-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] X線吸収分光法による蛇紋岩質土壌のNiとCrの化学形態分析2010

    • Author(s)
      橋本洋平・水野隆文
    • Organizer
      土壌肥料学会2010年度大会
    • Place of Presentation
      札幌(北海道大学)
    • Year and Date
      2010-09-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 北海道の廃止鉱山域河川におけるヤナギ類および水辺樹木の重金属集積2010

    • Author(s)
      水野隆文・佐藤千芳・水野直治
    • Organizer
      土壌肥料学会2011年度大会
    • Place of Presentation
      つくば国際会議場(つくば)
    • Year and Date
      2010-08-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] 重金属集積植物Thlaspi japonicum由来重金属トランスポーターの機能解析2010

    • Author(s)
      西田翔・愛須彩加・水野隆文
    • Organizer
      土壌肥料学会2011年度大会
    • Place of Presentation
      つくば国際会議場(つくば)
    • Year and Date
      2010-08-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Characterization of alternative splice variants of TjZNT1, a Zrt/Irt-like protein family metal transporter gene, of Thlaspi japonicum2010

    • Author(s)
      Sho Nishida, Ayaka Aisu, Takafumi Mizuno
    • Organizer
      Plant Biology 2010
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      2010-08-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] X線吸収分光法による蛇紋岩質土壌のNiとCrの化学形態分析2010

    • Author(s)
      橋本洋平・水野隆文
    • Organizer
      日本土壌肥料学会2010大会
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物の亜鉛吸収経路を介したニッケルの過剰吸収2010

    • Author(s)
      西田翔・加藤亜季・都築千里・愛須彩加・鰐部順平・小畑仁・水野隆文
    • Organizer
      日本土壌肥料学会2010大会
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of alternative splice variants of TjZNT1, a Zrt/Irt-like protein family metal transporter gene, of Thlaspi japonicum2010

    • Author(s)
      Sho Nishida, Ayaka Aisu and Takafumi Mizuno
    • Organizer
      Plant Biology 2010, the Montreal convention centre
    • Place of Presentation
      Montreal, Canada
    • Related Report
      2011 Final Research Report
  • [Presentation] マンガン超集積性植物コシアブラのファイトマイニングへの利用2010

    • Author(s)
      水野隆文, 江守香苗, 橋本洋平
    • Organizer
      日本植物生理学会第51回年会
    • Place of Presentation
      熊本大学(熊本)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物の鉄吸収経路を介したニッケルの過剰吸収2010

    • Author(s)
      西田翔、加藤亜紀、都築知里、水野隆文
    • Organizer
      日本植物生理学会第51回年会
    • Place of Presentation
      熊本大学(熊本)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物におけるニッケル吸収機構の解明2009

    • Author(s)
      西田翔・都築知里・加藤亜季・小畑仁・水野隆文
    • Organizer
      日本土壌肥料学会講演要旨集第55集
    • Place of Presentation
      京都(京都大学)
    • Year and Date
      2009-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] 日本の重金属超集積性植物-主にNiとMnについて-2009

    • Author(s)
      水野隆文
    • Organizer
      第27回日本植物細胞分子生物学会(藤沢)大会
    • Place of Presentation
      日本大学(神奈川)
    • Year and Date
      2009-07-31
    • Related Report
      2011 Final Research Report
  • [Presentation] 日本の重金属超集積性植物-主にNiとMnについて-2009

    • Author(s)
      水野隆文
    • Organizer
      第27回日本植物細胞分子生物学会
    • Place of Presentation
      日本大学(神奈川)
    • Year and Date
      2009-07-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物におけるニッケル吸収機構の解明2009

    • Author(s)
      西田翔・都築知里・加藤亜季・小畑仁・水野隆文
    • Organizer
      日本土壌肥料学会2009年度大会
    • Place of Presentation
      京都大学(京都)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 複合重金属汚染条件における緑化用ソバの鉛集積能力の検討2009

    • Author(s)
      森友梨恵・堀田あゆみ・加藤温子・田村英生・坂野克久・水野隆文
    • Organizer
      日本土壌肥料学会2009年度大会
    • Place of Presentation
      京都大学(京都)
    • Related Report
      2009 Annual Research Report
  • [Book] 植物栄養学2010

    • Author(s)
      水野隆文
    • Publisher
      文永堂出版
    • Related Report
      2011 Final Research Report
  • [Book] 環境科学2010

    • Author(s)
      水野隆文
    • Publisher
      オーム社
    • Related Report
      2011 Final Research Report
  • [Book] 地球環境テキストブック 環境科学2010

    • Author(s)
      水野隆文, ほか
    • Publisher
      オーム社(In press)
    • Related Report
      2009 Annual Research Report
  • [Book] 植物栄養学2010

    • Author(s)
      水野隆文, ほか
    • Publisher
      文永堂(In press)
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.bio.mie-u.ac.jp/junkan/busshitsu/lab4/SSPN/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.bio.mie-u.ac.jp/junkan/busshitsu/lab4/SSPN/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.bio.mie-u.ac.jp/junkan/busshitsu/lab4/SSPN/index.html.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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