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For the improvement of drought tolerance in Jatropha curcas

Research Project

Project/Area Number 21780055
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant nutrition/Soil science
Research InstitutionOsaka University

Principal Investigator

SHIBAGAKI Nakako  Osaka University, 工学研究科, 寄附講座准教授 (50525152)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords植物代謝調節 / バイオディーゼル / ジャトロファ / 乾燥ストレス / 形質転換
Research Abstract

Jatropha curcas L. is one of the promising oil plants as a feed stock for future biodiesel production in the world. Despite the fact that J.curcas is believed to grow and yield oil even in semi-dry area, its physiological responses to water defit has not been studied at molecular level. In this work, we successfully determined the changes in profile of primary metabolite accumulation and gene expression upon drought in J.curcas. Physiological responses, such as stomatal conductance, growth, water and ABA content in leaf, were also examined. Some metabolic pathways and genes were suggested as possible targets for genetic improvement aiming to enhance Jatropha's drought tolerance. To perform such genetic manipulation, we also investigated various conditions for efficient transformation of J.curcas using Agrobacterium tumefaciens in this work.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (32 results)

All 2011 2010 2009 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (13 results) Book (1 results) Remarks (2 results) Patent(Industrial Property Rights) (8 results)

  • [Journal Article] Sequence analysis of the genome of an oil-bearing tree Jatropha curcas2011

    • Author(s)
      Sato S, …(25) …, Shibagaki N, …(7).
    • Journal Title

      DNA Research Epub, ahead of print, (Dec 13, 2010)(IF 4.917) 18(1)

      Pages: 65-76

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sequence analysis of the genome of an oil-bearing tree, Jatropha curcas2011

    • Author(s)
      Sato S, ……, Shibagaki N, … Tabata S., (32名)
    • Journal Title

      DNA Research

      Volume: 18(1) Pages: 65-76

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adventitious Shoot Regeneration from Juvenile Cotyledons of a Biodiesel Producing Plant, Jatropha curcas L.2011

    • Author(s)
      Khemkladngoen N, Cartagena J, Shibagaki N, Fukui K
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 111(1) Pages: 67-70

    • NAID

      110008440352

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adventitious Shoot Regeneration from Juvenile Cotyledons of a Biodiesel Producing Plant, Jatropha curcas L.2010

    • Author(s)
      Khemkladngoen N, Cartagena J, Shibagaki N, Fukui K
    • Journal Title

      Journal of Bioscience and Bioengineering Epub 2010 Oct 2(2011-01) 111(1)

      Pages: 67-70

    • NAID

      110008440352

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Binding of cysteine synthase to the STAS domain of sulfate transporter and its regulatory consequences2010

    • Author(s)
      Shibagaki N, Grossman AR.
    • Journal Title

      Journal of Biological Chemistry 2010 Aug 6(Epub 2010 Jun 7, Times cited 2)(IF 5.33) 285(32)

      Pages: 25094-102

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Binding of cysteine synthase to the STAS domain of sulfate transporter and its regulatory consequences2010

    • Author(s)
      Shibagaki N, Grossman AR
    • Journal Title

      Journal of Biological Chemistry

      Volume: 285(32) Pages: 25094-102

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Physical mapping of ribosomal DNA genes on Jatropha curcas chromosomes by multicolor FISH2009

    • Author(s)
      Witkowska M, Ohmido N, Cartagena J, Shibagaki N, Fukui K.
    • Journal Title

      Cytologia Vol.74 No.2

      Pages: 133-139

    • NAID

      10025405446

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Physical mapping of ribosomal DNA genes on Jatropha curcas chromosomes by multicolor FISH2009

    • Author(s)
      Witkowska M, Ohmido N, Cartagena J, Shibagaki N, Fukui K
    • Journal Title

      Cytologia

      Volume: 74,2 Pages: 133-139

    • NAID

      10025405446

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] NTSセミナー2011

    • Author(s)
      柴垣奈佳子
    • Organizer
      文京区、東京(招待講演)
    • Place of Presentation
      文京区、東京(招待講演)
    • Year and Date
      2011-03-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Genetic Engineering of Jatropha curcas L.for improvement of drought resistance2010

    • Author(s)
      Shibagaki, N.
    • Organizer
      3rd Aachen-Osaka Joint Symposium on Biological and Chemical Approaches to Selective Processes at RWTH Aachen
    • Place of Presentation
      Germany(Invited talk)
    • Year and Date
      2010-09-07
    • Related Report
      2010 Final Research Report
  • [Presentation] Genetic Engineering of Jatropha curcas L.for improvement of drought resistance2010

    • Author(s)
      Nakako Shibagaki
    • Organizer
      3^<rd> Aachen-Osaka Joint Symposium on Biological and Chemical Approaches to Selective Processes
    • Place of Presentation
      RWTH Aachen, Aachen, Germany
    • Year and Date
      2010-09-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Regulation of sulfate transport by interaction between sulfate transporter and cysteine synthase2010

    • Author(s)
      Nakako Shibagaki-presenter, Arthur R Grossman (oral)
    • Organizer
      8^<th> International Workshop Sulfur metabolism in higher plants at the University of Melbourne
    • Place of Presentation
      Creswick, Victoria, Australia
    • Related Report
      2010 Final Research Report
  • [Presentation] Changes in the profiles of primary metabolite accumulation upon drought stress in Jatropha curcas L.2010

    • Author(s)
      Nakako Shibagaki-presenter, Hiroshi Tsugawaa, Eiichiro Fukusaki (poster)
    • Organizer
      Annual Meeting of American Society of Plant Biologist at Congress Center
    • Place of Presentation
      Montreal, Quebec, Canada
    • Related Report
      2010 Final Research Report
  • [Presentation] Interaction between a sulfate transporter and cysteine synthase2010

    • Author(s)
      Nakako Shibagaki-presenter, Arthur R Grossman (poster)
    • Organizer
      International Conference on Arabidopsis Research at Pacifico Yokohama
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] Regulation of sulfate transport by interaction between sulfate transporter and cysteine synthase2010

    • Author(s)
      Nakako Shibagaki-presenter, Arthur R Grossman
    • Organizer
      8^<th> International Workshop Sulfur metabolism in higher plant
    • Place of Presentation
      the University of Melbourne, Creswick, Victoria, Australia
    • Related Report
      2010 Annual Research Report
  • [Presentation] Changes in the profiles of primary metabolite accumulation upon drought stress in Jatropha curcas L.2010

    • Author(s)
      Nakako Shibagaki-presenter Hiroshi Tsugawa, Eiichiro Fukusaki
    • Organizer
      Annual Meeting of American Society of Plant Biologist
    • Place of Presentation
      Congress Center, Montreal, Quebec, Canada
    • Related Report
      2010 Annual Research Report
  • [Presentation] Interaction between a sulfate transporter and cysteine synthase2010

    • Author(s)
      Nakako Shibagaki-presenter, Arthur R Grossman
    • Organizer
      International Conference on Arabidopsis Research
    • Place of Presentation
      Pacifico Yokohama, Yokohama, Japan
    • Related Report
      2010 Annual Research Report
  • [Presentation] 油糧植物ジャトロファの改良に向けて-日本における遺伝子改変のための基盤整備と方向性ー2009

    • Organizer
      日本生物工学会大会シンポジウム(を、開催。以下の題名で講演。「半乾燥地における生産性の高い遺伝子改変型ジャトロファの作製を目指して」)
    • Year and Date
      2009-09-25
    • Related Report
      2010 Final Research Report
  • [Presentation] 半乾燥地における生産性の高い遺伝子改変型ジャトロファの作製を目指して2009

    • Author(s)
      Nakako Shibagaki, Joyce Cartagena, Satoshi Tabata, Yasuo Kanematsu, Kzuyoshi Itoh, Kiichi Fukui
    • Organizer
      日本生物工学会年会シンポジウム
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヤトロファにおける遺伝解析と遺伝子改変による改良2009

    • Author(s)
      柴垣奈佳子
    • Organizer
      Biofuels World 2009 招待講演
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-07-23
    • Related Report
      2010 Final Research Report
  • [Presentation] ヤトロファにおける遺伝解析と遺伝子改変による改良2009

    • Author(s)
      Nakako Shibagaki
    • Organizer
      Biofuels World 2009
    • Place of Presentation
      パシフィコ横浜(招待講演)
    • Year and Date
      2009-07-23
    • Related Report
      2009 Annual Research Report
  • [Book] 「バイオディーゼルの開発」福住俊一監修「環境を支える科学技術」2011

    • Author(s)
      Fukui K, Katakura Y, Shibagaki N 分担執筆
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www-bie-sei.mls.eng.osaka-u.ac.jp/home/index.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.honjodesign.jp/handai2011/pdf/12010110.pdf

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 遺伝子導入植物体の形成方法2010

    • Inventor(s)
      柴垣奈佳子、Suthitar Singkaravanit、池口直樹、湯塩泰久
    • Industrial Property Rights Holder
      柴垣奈佳子、Suthitar Singkaravanit、池口直樹、湯塩泰久
    • Industrial Property Number
      2010-262581
    • Filing Date
      2010-11-25
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Patent(Industrial Property Rights)] アグロバクテリウム媒介形質転換方法及びこれを用いた形質転換植物の作出方法2010

    • Inventor(s)
      柴垣奈佳子、Suthitar Singkaravanit、池口直樹、湯塩泰久
    • Industrial Property Rights Holder
      柴垣奈佳子、Suthitar Singkaravanit、池口直樹、湯塩泰久
    • Industrial Property Number
      2010-261982
    • Filing Date
      2010-11-25
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Patent(Industrial Property Rights)] 植物の遺伝子導入方法及び形質転換植物の作出方法2010

    • Inventor(s)
      柴垣奈佳子、Suthitar Singkaravanit、池口直樹、湯塩泰久
    • Industrial Property Rights Holder
      柴垣奈佳子、Suthitar Singkaravanit、池口直樹、湯塩泰久
    • Industrial Property Number
      2010-262180
    • Filing Date
      2010-11-25
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Patent(Industrial Property Rights)] ジャトロファ属植物の栽培方法2010

    • Inventor(s)
      柴垣奈佳子、津川裕司、福崎英一郎、ジョイス カルタヘナ、福井希一
    • Industrial Property Rights Holder
      柴垣奈佳子、津川裕司、福崎英一郎、ジョイス カルタヘナ、福井希一
    • Industrial Property Number
      2010-066359
    • Filing Date
      2010-03-24
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 組織培養法2010

    • Inventor(s)
      柴垣奈佳子、ナルモンケムクラーゲン、ジョイスカルタヘナ、福井希一
    • Industrial Property Rights Holder
      柴垣奈佳子、ナルモンケムクラーゲン、ジョイスカルタヘナ、福井希一
    • Industrial Property Number
      2010-003150
    • Filing Date
      2010-01-08
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 組織培養法2010

    • Inventor(s)
      柴垣奈佳子、Naruemon Khemkladgoen、Joyce Cartagena、福井希
    • Industrial Property Number
      2010-235603
    • Filing Date
      2010-10-20
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] ジャトロファ属植物の栽培方法2010

    • Inventor(s)
      柴垣奈佳子、津川裕司、福崎英一郎、Joyce Cartagena、福井希一
    • Industrial Property Rights Holder
      柴垣奈佳子、津川裕司、福崎英一郎、Joyce Cartagena、福井希一
    • Filing Date
      2010-03-24
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] 組織培養法2010

    • Inventor(s)
      柴垣奈佳子、Naruemon Khemkladgoen、Joyce Cartagena、福井希一
    • Industrial Property Rights Holder
      柴垣奈佳子、Naruemon Khemkladgoen、Joyce Cartagena、福井希一
    • Filing Date
      2010-10-20
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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