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Identification of the expression mechanism of the virulence factors by quorum sensing in plant pathogen and its application

Research Project

Project/Area Number 21780063
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionUtsunomiya University

Principal Investigator

MOROHOSHI Tomohiro  宇都宮大学, 大学院・工学研究科, 助教 (90361360)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsクオラムセンシング / アシル化ホモセリンラクトン / 植物病原菌 / Pantoea ananatis / quorum sensing / acylhomoserine lactone / plant pathogen / 転写制御 / 菌体凝集 / アドヘシン / 菌体外多糖 / 病原性因子
Research Abstract

Many plant pathogens have a quorum-sensing system, a cell-cell communication mechanism that depends on cell population density. Several kinds of N-acyl homoserine lactone (AHL) have been identified as signal compounds involved in this mechanism. In this study, we attempted to identify the unknown factors, which were regulated by AHL-mediated quorum sensing in the plant pathogen Pantoea ananatis SK-1. As the results, it was revealed that the production of extracellular polysaccharide (EPS) and cell aggregation were regulated by AHL-mediated quorum sensing in P. ananatis SK-1.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (16 results)

All 2012 2011 2010 2009 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (11 results) Remarks (1 results)

  • [Journal Article] Exopolysaccharide production is influenced by sugars, N-acyl homoserine lactone and transcriptional regulators RcsA and RcsB, but does not affect pathogenicity in the plant pathogen Pantoea ananatis2011

    • Author(s)
      T. Morohoshi, K. Oseki, T. Ikeda
    • Journal Title

      Biosci. Biotech. Biochem.

      Volume: 75 Pages: 997-999

    • NAID

      10028273007

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Exopolysaccharide Production Is Influenced by Sugars, N-Acylhomoserine Lactone, and Transcriptional Regulators RcsA and RcsB, but Does Not Affect Pathogenicity in the Plant Pathogen Pantoea ananatis2011

    • Author(s)
      T.Morohoshi, K.Oseki, T.Ikeda
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Issue: 5 Pages: 997-999

    • DOI

      10.1271/bbb.100888

    • NAID

      10028273007

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell aggregation is negatively regulated by N-acylhomoserine lactone-mediated quorumsensing in Pantoea ananatis SK-12011

    • Author(s)
      T.Morohoshi, Y.Ogata, T.Ikeda
    • Journal Title

      J.Biosci.Bioeng.

      Volume: 112 Issue: 6 Pages: 566-569

    • DOI

      10.1016/j.jbiosc.2011.08.004

    • NAID

      110008897330

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell aggregation is negatively regulated by N-acyl homoserine lactone-mediated quorum sensing in Pantoea ananatis SK-12010

    • Author(s)
      T. Morohoshi, Y. Ogata, T. Ikeda
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 112 Pages: 566-569

    • NAID

      110008897330

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] Pantoea ananatisのQuorum Sensingに制御される病原性因子の探索2012

    • Author(s)
      諸星知広,菊池健太,緒方優二,上岡夏海,池田宰
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Cell aggregation is regulated by N-acylhomoserine lactone-mediated quorum sensing in the plant pathogen Pantoea ananatis2011

    • Author(s)
      T.Morohoshi, Y.Ogata, T.Ikeda
    • Organizer
      Asian Congress on Biotechnology 2011
    • Place of Presentation
      Shanghai Everbright Convention & Exhibition Centre
    • Year and Date
      2011-05-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cell aggregation is regulated by N-acyl homoserine lactone-mediated quorum sensing in the plant pathogen Pantoea ananatis2011

    • Author(s)
      T. Morohoshi, Y. Ogata, T. Ikeda
    • Organizer
      Asian Congress on Biotechnology 2011 (ACB-2011)
    • Place of Presentation
      Shanghai, China
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物病原菌Pantoea ananatisにおける菌体凝集のクオラムセンシングによる制御2011

    • Author(s)
      諸星知広,緒方優二,池田宰
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] The production of carotenoid pigments is regulated by acylhomoserine lactone-mediated quorum sensing in Pantoea ananatis2010

    • Author(s)
      N.Kamioka, T.Morohoshi, T.Ikeda
    • Organizer
      2010 International Chemical Congress of Pacific Basin Societies (Pacifichem 2010)
    • Place of Presentation
      Hawaii Convention Center (Honolulu, USA)
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物病原菌 Pantoea ananatis の菌体外多糖生産に関わる遺伝子群の解析2010

    • Author(s)
      諸星知広, 大関貴恵美, 池田宰
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学駒場キャンパス
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] The production of carotenoid pigments is regulated by acylhomoserine lactone-mediated quorum sensing in Pantoea ananatis2010

    • Author(s)
      N. Kamioka, T. Morohoshi, T. Ikeda
    • Organizer
      2010 International Chemical Congress of Pacific Basin Societies (Pacifichem 2010)
    • Place of Presentation
      Honolulu, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物病原菌Pantoea ananatisにおける菌体凝集のクオラムセンシングによる制御2010

    • Author(s)
      緒方優二,諸星知広,池田宰
    • Organizer
      日本生物工学会2010年度大会
    • Place of Presentation
      宮崎シーガイア
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 植物病原菌Pantoea ananatisの菌体外多糖生産に関わる遺伝子群の解析2010

    • Author(s)
      諸星知広,大関貴恵美,池田宰
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物病原菌 Pantoea ananatis のクオラムセンシングによる菌体外多糖生産制御機構の解析2009

    • Author(s)
      大関貴恵美, 諸星知広, 池田宰
    • Organizer
      日本生物工学会2009年度大会
    • Place of Presentation
      名古屋大学東山キャンパス
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物病原菌Pantoea ananatisのクオラムセンシングによる菌体外多糖生産制御機構の解析2009

    • Author(s)
      大関貴恵美,諸星知広,池田宰
    • Organizer
      日本生物工学会2009年度大会
    • Place of Presentation
      名古屋大学
    • Related Report
      2011 Final Research Report
  • [Remarks] 生物工学研究室ホームページ

    • URL

      http://www.chem.utsunomiya-u.ac.jp/lab/bio/

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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