| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
MsiK is an ATP-binding cassette protein, which is essential for uptake of some oligosaccharides in streptomycetes. In this study, we successfully obtained proteins interacting MsiK by pull-down method, Blue-Native PAGE, and subsequent mass spectrometry analysis. One of the gained proteins, of which transport substrates are unknown, was suggested to possess novel binding specificity.
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