Exploration and characterization of novel transporters for oligosaccharides and polyols in actinomycetes
| Project/Area Number |
21780064
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Shizuoka Institute of Science and Technology (2010-2011)
Chiba University (2009) |
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Principal Investigator |
SAITO Akihiro 静岡理工科大学, 理工学部, 准教授 (50375614)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ABCトランスポーター / 放線菌 / 糖質輸送系 / トランスポーター / 糖質 / キチン / BN-PAGE / PMFP |
|---|
| Research Abstract |
MsiK is an ATP-binding cassette protein, which is essential for uptake of some oligosaccharides in streptomycetes. In this study, we successfully obtained proteins interacting MsiK by pull-down method, Blue-Native PAGE, and subsequent mass spectrometry analysis. One of the gained proteins, of which transport substrates are unknown, was suggested to possess novel binding specificity.
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] Molecular and enzymatic characterization of a subfamily I.4 lipase from an edible oil degrader Bacillus sp. HH-012011
Author(s)
Kamijo, T., Saito, A., Ema, S., You, I., Hayashi, H., Nagata, R., Nagata, Y., Ando, A.
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Journal Title
Anton. Leeuw. Int. J . G
Volume: 99
Issue: 2
Pages: 179-187
DOI
Related Report
Peer Reviewed
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[Journal Article] Streptomyces coavervatus sp.nov., isolated from the intestinal tract of Armadillidium vulgae2011
Author(s)
Shibasaki, A., Kudo, T., Yaguchi, T., Saito, A., Ando, A., Mikami, Y., Gonoi, T.
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Journal Title
International Journal of Systematic and Environmental Microbiology
Volume: 61
Pages: 1073-1077
Related Report
Peer Reviewed
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[Presentation] Chemical structures, syntheses and applications of Mycoplasma pneumoniae-specific glycolipid antigens2010
Author(s)
Nishida Y, Matsuda K, Koizumi A, Fukuda K, Dohi H, Harasawa R, Ichiyama K, Matsuda S, Saito A, Ebise H, Tokishige R
Organizer
25^<th> International Carbohydrate Symposium
Place of Presentation
Makuhari Messe, Chiba, Japan
Year and Date
2010-08-04
Related Report
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