Isolation of RNA polymerase mutants of actinomycetes through the acquisition of drug resistance and discovery novel antibiotics
| Project/Area Number |
21780068
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Shinshu University |
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Principal Investigator |
HOSAKA Takeshi 信州大学, ファイバーナノテク国際若手研究者育成拠点, 助教 (50391206)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 抗生物質生産 / 微生物潜在機能 / 薬剤耐性変異 / RNAポリメラーゼ / 放線菌 / 二次代謝産物生合成遺伝子 / 潜在遺伝子 |
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| Research Abstract |
This study demonstrated the pragmatic method for efficiently activating unexpressed or poorly expressed actinomycetes gene to synthesize secondary metabolites through a mutation that confers resistance to rifampicin targeting the RNA polymerase ; a specific mutation in the rpoB gene(encoding the RNA polymeraseβ-subunit) dramatically altered gene expression in actinomycetes by modulating the transcriptional apparatus(i. e., RNA polymerase). This method allowed the discovery of piperidamycin, a novel antibiotic produced by Streptomyces sp 631689, which rarely produces antibiotics in any type of culture media.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Antibacterial discovery in actinomycetes strains with mutations in RNA polymerase or ribosomal protein S122009
Author(s)
Hosaka, T., Ohnishi-Kameyama, M., Muramatsu, H., Murakami, K., Tsurumi, Y., Kodani, S., Yoshida, M., Fujie, A. and Ochi, K.
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Journal Title
Nature Biotechnology
Volume: Vol.27
Issue: 5
Pages: 462-464
DOI
Related Report
Peer Reviewed
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[Journal Article] Bacterial alarmone, guanosine 5'-diphosphate 3'-diphosphate(ppGpp), predominantly binds the beta' subunit of plastid-encoded plastid RNA polymerase in chloroplasts2009
Author(s)
Sato, M., Takahashi, K., Ochiai, Y., Hosaka, T., Ochi, K. and Nabeta, K.
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Journal Title
Chem Bio Chem
Volume: Vol.10
Issue: 7
Pages: 1227-1233
DOI
Related Report
Peer Reviewed
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