| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
This study demonstrated the pragmatic method for efficiently activating unexpressed or poorly expressed actinomycetes gene to synthesize secondary metabolites through a mutation that confers resistance to rifampicin targeting the RNA polymerase ; a specific mutation in the rpoB gene(encoding the RNA polymeraseβ-subunit) dramatically altered gene expression in actinomycetes by modulating the transcriptional apparatus(i. e., RNA polymerase). This method allowed the discovery of piperidamycin, a novel antibiotic produced by Streptomyces sp 631689, which rarely produces antibiotics in any type of culture media.
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