| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Research Abstract |
Due to the difference of the physiological roles between NAD(H) and NADP(H) as well as the significant impacts of them on the cellular metabolism, it is important to elucidate the mechanism underlying the regulation of biosynthesis and degradation of NADP(H). In this study, the tertiary structure of budding yeast NADPH-biosynthetic enzyme (NADH kinase ; Pos5) complexed with NADH was determined at 2.0Å. Detailed analysis including the comparison of the tertiary structure of Pos5 with those of human and bacterial NADP^+-biosynthetic enzymes (NAD kinases) revealed that the Pos5 Arg293 is at least one of the structural determinants of Pos5 for high NADH kinase activity and for discriminating NADH from NAD^+.
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