Genome-wide screening of essential genes showing haplo-insufficiency in secretory protein production in yeast
| Project/Area Number |
21780076
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
HOSHIDA Hisashi 山口大学, 大学院・医学系研究科, 准教授 (00314823)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 分泌性タンパク質生産 / 必須遺伝子 / ハプロ不全 / Saccharomyces cerevisiae / 必須遺伝子ヘテロ破壊株 / 分泌性ルシフェラーゼ / 必須遺伝子ヘテロ破壊株セット / 必須遺伝子へテロ破壊株セット |
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| Research Abstract |
To identify the genes affecting heterologous gene expression in Saccharomyces cerevisiae, heterozygous knockout strains of essential genes were used. As a result, 7 mutants that enhance expression of a secretory luciferase were identified. On the other hand none of knockout strain showed significantly lower expression except for weak-growth mutants. Six genes among the knockout strains were involved in RNA metabolism.
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Report
(4 results)
Research Products
(4 results)
