Genome-wide screening of essential genes showing haplo-insufficiency in secretory protein production in yeast

Research Project

Project/Area Number	21780076
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Applied microbiology
Research Institution	Yamaguchi University
Principal Investigator	HOSHIDA Hisashi 山口大学, 大学院・医学系研究科, 准教授 (00314823)
Project Period (FY)	2009 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	分泌性タンパク質生産 / 必須遺伝子 / ハプロ不全 / Saccharomyces cerevisiae / 必須遺伝子ヘテロ破壊株 / 分泌性ルシフェラーゼ / 必須遺伝子ヘテロ破壊株セット / 必須遺伝子へテロ破壊株セット
Research Abstract	To identify the genes affecting heterologous gene expression in Saccharomyces cerevisiae, heterozygous knockout strains of essential genes were used. As a result, 7 mutants that enhance expression of a secretory luciferase were identified. On the other hand none of knockout strain showed significantly lower expression except for weak-growth mutants. Six genes among the knockout strains were involved in RNA metabolism.

Report

(4 results)