| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Research Abstract |
Cysteine desulfurase is a PLP-dependent enzyme and generates sulfur atom for the biosynthesis of sulfur-containing compounds, such as thiamine, biotin, and FeS cluster. We purified three cysteine desulfurases from obligate anaerobe, Clostridium acetobutylicum, and characterized their enzymatic properties. CAC2234 showed highest reactivity on L-Cys, we thus used them to examine the sulfur transfer from L-[^<35> S] Cys. We found five proteins specifically time-dependently labeled by^<35> S.
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