LitR-mediated photoresponse in non-phototrophic bacteria : studies on its molecular mechanism and potential diversity

• Project/Area Number: 21780083
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Nihon University
• Principal Investigator: TAKANO Hideaki Nihon University, 生物資源科学部, 助教 (50385994)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: Photo-response / Transcriptional regulation / LitR / MerR family / Cobalamin / Streptomyces coelicolor / Thermus thermophilus / Pseudomonas putida / 光応答 / コバラミン / 転写調節

Research Abstract

The homologous gene of Light-responsive transcriptional regulator (LitR) is distributed in gram positive and gram negative bacteria. In this study, we revealed that LitR homologs of Thermus thermophilus and Pseudomonas putida are also a central regulator for photo-inducible carotenogenesis and the transcriptional regulation of novel light-inducible genes, respectively.

Research Products

• [Journal Article] Involvement of CarA/LitR and CRP/FNR family transcriptional regulators in light-induced carotenoid production in Thermus thermophilus. (2011)
  • Author(s): Takano H, Kondo M, Usui N, Usui T, Ohzeki H, Yamazaki R, Washioka M, Nakamura A, Hoshino T, Hakamata W, Beppu T, Ueda K
  • Journal Title: Journal of Bacteriology 193 Pages: 2451-2459
  • Peer Reviewed
• [Journal Article] Involvement of CarA/LitR and CRP/FNR family transcriptional regulators in light-induced carotenoid production in Thermus thermophilus. (2011)
  • Author(s): Takano, et al.
  • Journal Title: J.Bacteriol. Volume: (In press)
  • Peer Reviewed
• [Journal Article] 光センサー機能をもつ新しいタイプの転写調節因子LitR (2010)
  • Author(s): 高野英晃, ら
  • Journal Title: 化学と生物 Volume: 48 Pages: 82-83
• [Presentation] 高度好熱菌Thermus thermophilusにおける光応答性遺伝子群の網羅的探索とその制御機構の解明 (2011)
  • Author(s): 高野英晃、上利佳弘、山崎竜大、新海暁男、上田賢志
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-27
• [Presentation] Pseudomonas putidaの光応答:新規な光誘導性遺伝子群とその転写制御機構 (2011)
  • Author(s): 牟田口尚敬、高野英晃、新谷政己、野尻秀昭、上田賢志
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-27
• [Presentation] 原核生物だけがつくるビタミン、VB12:一部の菌にみられる細胞外蓄積現象 (2011)
  • Author(s): 萩原健太、高野英晃、上田賢志
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-26
• [Presentation] 高度好熱菌Thermus thermophilusの巨大プラスミドに集約された光応答性遺伝子群の発現制御メカニズム (2011)
  • Author(s): 高野英晃、上利佳弘、山崎竜大、新海暁男、上田賢志
  • Organizer: 日本ゲノム微生物学会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-16
• [Presentation] Thermus thermophilus HB27の光応答 (2010)
  • Author(s): 高野英晃、山崎竜大、袴田航、中村顕、星野貴行、別府輝彦、上田賢志
  • Organizer: 高度好熱菌丸ごと一匹プロジェクト第9会連携研究会
  • Place of Presentation: 播磨
  • Year and Date: 2010-08-21
• [Presentation] Thermus thermophilusの光応答 (2010)
  • Author(s): 山崎竜大、高野英晃、上田賢志
  • Organizer: 微生物研究会「微生物分子生物学の新たな挑戦」
  • Place of Presentation: 東京
  • Year and Date: 2010-06-26
• [Presentation] Pseudomonas putidaの光応答 (2010)
  • Author(s): 牟田口尚敬、高野英晃、上田賢志
  • Organizer: 微生物研究会「微生物分子生物学の新たな挑戦」
  • Place of Presentation: 東京
  • Year and Date: 2010-06-26
• [Presentation] Thermus thermoplilusが示す光誘導性カロテノイド生産の分子メカニズム (2010)
  • Author(s): 高野, ら
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京(東大・駒場)
  • Year and Date: 2010-03-28
• [Presentation] Thermus thermophilusの光誘導性カロテノイド生産に関わるcAMPレセプター様蛋白の解析 (2010)
  • Author(s): 高野, ら
  • Organizer: 第4回日本ゲノム微生物学会年会
  • Place of Presentation: 九州大学(博多)
  • Year and Date: 2010-03-08
• [Presentation] 細菌群集の光応答:ビタミンB12が果たすグローバルな役割 (2009)
  • Author(s): 高野, ら
  • Organizer: 日本農芸化学会2009年度関東支部大会
  • Place of Presentation: 玉川大学(東京・町田)
  • Year and Date: 2009-10-31