Functional Analysis of RNA helicases in regulation of miRNA processing

• Project/Area Number: 21780091
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: FUJIYAMA Sally The University of Tokyo, 分子細胞生物学研究所, 助教 (50447372)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 遺伝子発現 / マイクロRNA / RNAヘリカーゼ / ショウジョウバエ遺伝学

Research Abstract

RNA helicase are enzymes to modulate the structures of double strand RNA and RNA-protein and are classified into families by their functional domains. There are several hundred kinds of putative RNA helicases, however, functions of only a little kind of helicases are elucidated. Thus, in this study I try to clarify the biological functions and reaction mechanisms of the novel RNA helicases by combination of Drosophila genetics and proteomic analysis.

Research Products

• [Journal Article] A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing. (2011)
  • Author(s): Ishiguro K, Kim J, Fujiyama-Nakamura S, Kato S, Watanabe Y
  • Journal Title: EMBO Rep. vol.12 Pages: 267-275
  • Peer Reviewed
• [Journal Article] A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing. (2011)
  • Author(s): Ishiguro K, Kim J, Fujiyama-Nakamura S, Kato S, Watanabe Y
  • Journal Title: EMBO Rep. Volume: 12 Pages: 267-275
  • Peer Reviewed
• [Journal Article] Identification of osteoclastic factors in the nuclear envelope of mature, multinucleated osteoclasts. (2010)
  • Author(s): Youn MY, Fujiyama-Nakamura S, Takada I, Imai Y, Kato S.
  • Journal Title: Biosci Biotechnol Biochem. vol.74 Pages: 1956-1959
  • Peer Reviewed
• [Journal Article] A histone chaperone, DEK, transcriptionally coactivates a nuclear receptor. (2010)
  • Author(s): Sawatsubashi S, Murata T, Lim J, Fujiki R, Ito S, Suzuki E, Tanabe M, Zhao Y, Kimura S, Fujiyama S, Ueda T, Umetsu D, Ito T, Takeyama K, Kato S.
  • Journal Title: Genes Dev. vol.24 Pages: 159-170
  • Peer Reviewed
• [Journal Article] Purification and identification of estrogen receptor alpha co-regulators in osteoclasts. (2010)
  • Author(s): Takada I, Tsuji N, Youn MY, Fujiyama S, Okada M, Imai Y, Kondo S, Kitakawa H, Yasuda H, Kato S.
  • Journal Title: Ann NY Acad.Sci. vol.1192 Pages: 201-207
  • Peer Reviewed
• [Journal Article] The metazoan ATAC and SAGA coactivator HAT complexes regulate different sets of inducible target genes. (2010)
  • Author(s): Nagy Z, Riss A, Fujiyama S, Krebs A, Orpinell M, Jansen P, Cohen A, Stunnenberg HG, Kato S, Tora L.
  • Journal Title: Cell Mol Life Sci. vol.67 Pages: 611-628
  • Peer Reviewed
• [Journal Article] CDP/Cut is an Osteoblastic Co-activator of the Vitamin D receptor (VDR). (2010)
  • Author(s): Ochiai E, Kitagawa K, Takada I, Fujiyama S, Sawatsubashi S, Kim MS, Mezaki Y, Tshushima Y, Takagi K, Azuma Y, Takeyama K, Yamaoka K, Kato S, Kamimura T
  • Journal Title: J.Bone.Mineral Research vol.25 Pages: 1157-1166
  • Peer Reviewed
• [Journal Article] Double PHD fingers protein DPF2 recognizes acetylated histones and suppresses the function of ERRα through HDAC. (2010)
  • Author(s): Matsuyama R, Takada I, Yokoyama A, Fujiyma-Nakamura S, Tsuji N, Kitagawa H, Fujiki R, Kim MS, Kouzu-Fujita M, Yano T, Kato S
  • Journal Title: J.Biol.Chem. vol.285 Pages: 18166-18176
  • Peer Reviewed
• [Journal Article] Identification of osteoclastic factors in the nuclear envelope of mature, multinucleated osteoclasts. (2010)
  • Author(s): Youn MY, Fujiyama-Nakamura S, Takada I, Imai Y, Kato S
  • Journal Title: Biosci Biotechnol Biochem. Volume: 74 Pages: 1956-1959
  • Peer Reviewed
• [Journal Article] Purification and identification of estrogen receptor alpha co-regulators in osteoclasts. (2010)
  • Author(s): Takada I, Tsuji N, Youn MY, Fujiyama S, Okada M, Imai Y, Kondo S, Kitakawa H, Yasuda H, Kato S
  • Journal Title: Ann N Y Acad Sci. Volume: 1192 Pages: 201-207
  • Peer Reviewed
• [Journal Article] A histone chaperone, DEK, transcriptionally coactivates a nuclear receptor (2010)
  • Author(s): Sawatsubashi S, Murata T, Lim J, Fujiki R, Ito S, Suzuki E, Tanabe M, Zhao Y, Kimura S, Fujiyama S, et.al.
  • Journal Title: Genes Dev. 24 Pages: 159-170
  • Peer Reviewed
• [Journal Article] The metazoan ATAC and SAGA coactivator HAT complexes regulate different sets of inducible target genes (2010)
  • Author(s): Nagy Z, Riss A, Fujiyama S, et.al.
  • Journal Title: Cell Mol.Life Sci. 67 Pages: 611-628
  • Peer Reviewed
• [Journal Article] Maturation of microRNA is hormonally regulated by a nuclear receptor. (2009)
  • Author(s): Yamagata K, Fujiyama S, Ito S, Ueda T, Murata T, Naitou M, Takeyama K, Minami Y, O'Malley BW, Kato S
  • Journal Title: Mol.Cell vol.36 Pages: 340-347
  • Peer Reviewed
• [Journal Article] DNA demethylation in hormone-induced transcriptional derepression. (2009)
  • Author(s): Kim MS, Kondo T, Takada I, Youn MY, Yamamoto Y, Takahashi S, Matsumoto T, Fujiyama S, Shirode Y, Yamaoka I, Kitagawa H, Takeyama K, Shibuya H, Ohtake F, Kato S
  • Journal Title: Nature vol.461 Pages: 1007-1012
  • Peer Reviewed
• [Journal Article] BTB Protein, dKLHL18/CG3571, serves as an adaptor subunit for a dCul3 ubiquitin ligase complex. (2009)
  • Author(s): Fujiyama-Nakamura S, Ito S, Sawatsubashi S, Yamauchi Y, Suzuki E, Tanabe M, Kimura S, Murata T, Isobe T, Takeyama K, Kato S
  • Journal Title: Genes Cells vol.14 Pages: 965-973
  • Peer Reviewed
• [Journal Article] Parvulin (Par14), a peptidyl prolyl cis-trans isomerase, is a novel rRNA-processing factor evolved in the metazoan lineage. (2009)
  • Author(s): Fujiyama-Nakamura S, Yoshikawa H, Homma K, Hayano T, Tsujimura-Takahashi T, Izumikawa K, Ishikawa H, Miyazawa N, Yanagida M, Miura Y, Shinkawa T, Yamauchi Y, Isobe T, Takahashi N
  • Journal Title: Mol.Cell.Proteomics vol.8 Pages: 1552-1565
  • Peer Reviewed
• [Journal Article] Maturation of microRNA is hormonally regulated by a nuclear receptor (2009)
  • Author(s): Yamagata K, Fujiyama S, et.al.
  • Journal Title: Molecular Cell 36 Pages: 340-347
  • Peer Reviewed
• [Journal Article] DNA demethylation in hormone-induced transcriptional derepression (2009)
  • Author(s): Kim MS, Kondo T, Takada I, Youn MY, Yamamoto Y, Takahashi S, Matsumoto T, Fujiyama S, et al.
  • Journal Title: Nature 461 Pages: 1007-1012
  • Peer Reviewed
• [Journal Article] BTB Protein, dKLHL18/CG3571, serves as an adaptor subunit for a dCul3 ubiquitin ligase complex (2009)
  • Author(s): Fujiyama-Nakamura S, Ito S, et.al.
  • Journal Title: Genes to Cells 14 Pages: 965-973
  • Peer Reviewed
• [Journal Article] Parvulin(Par14), a peptidyl prolyl cis-trans isomerase, is a novel rRNA-processing factor evolved in the metazoan lineage (2009)
  • Author(s): Fujiyama-Nakamura S, Yoshikawa H, et.al.
  • Journal Title: Mol.Cell.Proteomics 8 Pages: 1552-1565
  • Peer Reviewed
• [Presentation] 核内巨大分子量複合体を介したクロマチン構造調節機構の解明 (2011)
  • Author(s): 藤山(中村)沙理
  • Organizer: 転写研究会若手シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2011-02-18
• [Presentation] 核内巨大分子量複合体を介したクロマチン構造調節機構の解明 (2011)
  • Author(s): 藤山(中村)沙理
  • Organizer: 転写研究会 若手シンポジウム
  • Place of Presentation: 東北大学東京分室 会議室
  • Year and Date: 2011-02-18
• [Presentation] Identification and characterization of a D12 mega size complex (2010)
  • Author(s): Sally Fujiyama-Nakamura, Saya Ito, Shun Sawatsubashi, Eriko Suzuki, Masahiko Tanabe, Shuhei Kimura, Takashi Ueda, Takuya Murata, Ken-ichi Takeyama, Shigeaki Kato
  • Organizer: 14^<th> International Congress of Endocrinology
  • Place of Presentation: 京都
  • Year and Date: 2010-03-30
• [Presentation] Identification and characterization of a D12 mega size complex (2010)
  • Author(s): 中村(藤山)沙理, 伊藤紗弥, 沢津橋俊, 鈴木絵里子, 田辺真彦, 木村周平, 上田崇, 村田拓哉, 武山健一, 加藤茂明
  • Organizer: 国際内分泌学会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-03-30
• [Presentation] 遺伝学的アプローチとプロテオミクスを連携した新規活性化クロマチン構造調節因子D12の複合体解析 (2010)
  • Author(s): 中村沙理, 伊藤紗弥, 沢津橋俊, 鈴木絵里子, 田辺真彦, 木村周平, 上田崇, 村田拓哉, 松川紘之, 林珍仙, 武山健一, 加藤茂明
  • Organizer: 日本農芸化学学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] 遺伝学的アプローチとプロテオミクスを連携した新規活性化クロマチン構造調節因子D12の複合体解析 (2010)
  • Author(s): 中村(藤山)沙理, 伊藤紗弥, 沢津橋俊, 鈴木絵里子, 田辺真彦, 木村周平, 上田崇, 村田拓哉, 林珍仙, 武山健一, 加藤茂明
  • Organizer: 日本農芸化学学会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-03-28
• [Book] 新遺伝子工学ハンドブック(「質量分析計を用いたタンパク質複合体解析」) (2010)
  • Author(s): 藤山(中村)沙理, 加藤茂明
  • Publisher: 羊土社
• [Book] Regulation of MicroRNAs(Repression of pri-miRNA processing through Eralpha)(Editor ; Grosshans H.) (2010)
  • Author(s): Fujiyama-Nakamura S., Yamagata K., Kato S.
  • Publisher: Landes Bioscience
• [Book] 現代栄養学を理解するための分子生物学入門(「プロテオミクス」「遺伝子ノックダウン」) (2010)
  • Author(s): 藤山(中村)沙理著, 加藤茂明編
  • Publisher: 光生館
• [Book] 新遺伝子工学ハンドブック (2010)
  • Author(s): 藤山(中村)沙理, 加藤茂明
  • Total Pages: 5
  • Publisher: 羊土社
• [Book] Regulation of Micro RNAs(Repression of pri-miRNA processing through ERalpha部分) (2010)
  • Author(s): Fujiyama-Nakamura S., Yamagata K., Kato S
  • Total Pages: 13
  • Publisher: Landes Bioscience
• [Book] がん分子標的治療研究実践マニュアル(LC-MS/MS)(日本がん分子標的治療学会編) (2009)
  • Author(s): 藤山沙理, 加藤茂明著
  • Publisher: 金芳堂
• [Book] がん分子標的治療研究 実践マニュアル(LC-MS/MS部分) (2009)
  • Author(s): 藤山沙理, 加藤茂明
  • Total Pages: 6
  • Publisher: 金芳堂
• [Book] 現代栄養学を理解するための分子生物学入門(「プロテオミクス」「遺伝子ノックダウン」部分) (2009)
  • Author(s): 藤山(中村)沙理
  • Total Pages: 7
  • Publisher: 光生館
• [Remarks] ホームページ等
  • URL: http://www.iam.u-tokyo.ac.jp/bnsikato/index.html