| Project/Area Number |
21780102
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Japan Atomic Energy Agency |
|---|
Principal Investigator |
YAMADA Mitsugu Japan Atomic Energy Agency, 量子ビーム応用研究部門, 博士研究員 (80510924)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
|---|
| Keywords | 生体分子 / タンパク質 / 生物物理 / 酵素反応 / バイオテクノロジー / 構造生物 |
|---|
| Research Abstract |
Two DNA ligase (DR2069, DRB0100) are identified in a genome of Deinococcus radiodurans.
As a result of analysis, DR2069 was able to confirm DNA ligase activity, but DRB0100 was not able to confirm the DNA ligase activity.
On the other hand, we carried out a crystallographic analysis of the DdrA protein which protected DNA from digestive enzymes before ligation with the DNA ligase and succeeded in crystallization and a diffraction data collection.
Diffraction data were collected to 2.10 Å resolution and structural analysis are carrying out now.
|
