Structural and functional analysis of high efficient DNA ligation complex
| Project/Area Number |
21780102
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Japan Atomic Energy Agency |
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Principal Investigator |
YAMADA Mitsugu Japan Atomic Energy Agency, 量子ビーム応用研究部門, 博士研究員 (80510924)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 生体分子 / タンパク質 / 生物物理 / 酵素反応 / バイオテクノロジー / 構造生物 |
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| Research Abstract |
Two DNA ligase (DR2069, DRB0100) are identified in a genome of Deinococcus radiodurans.
As a result of analysis, DR2069 was able to confirm DNA ligase activity, but DRB0100 was not able to confirm the DNA ligase activity.
On the other hand, we carried out a crystallographic analysis of the DdrA protein which protected DNA from digestive enzymes before ligation with the DNA ligase and succeeded in crystallization and a diffraction data collection.
Diffraction data were collected to 2.10 Å resolution and structural analysis are carrying out now.
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Report
(3 results)
Research Products
(5 results)
