Establishment of new lipidomics approach

• Project/Area Number: 21780110
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Tokyo Metropolitan University (2011); Hamamatsu University School of Medicine (2009-2010)
• Principal Investigator: INOUE Naoko 首都大学東京, 人間健康科学研究科, 特任助教 (00509515)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 分析化学 / 脂質 / 薄層クロマトグラフィー / 質量分析 / 糖脂質 / リン脂質 / 分子種解析 / イメージング

Research Abstract

We successfully established the new lipidomics approach named "TLC-Blot-MALDI imaging" which visualizes the lipid pattern obtained from thin layer chromatography by imaging mass spectrometry which is new analytical skills.
In this study, we evaluated the validity of the technique, and also applied to the biological samples. As a result, we succeeded to detect very small amounts of lipids which hardly detected by conventional methods. Moreover, comprehensive analyses were also performed to know the lipid dynamics change with molecular species level.

Research Products

• [Journal Article] (2012)
  • Author(s): Chansela P, Goto-Inoue N, Zaima N, Hayasaka T, Sroyraya M, Kornthong N, Engsusophon A, Tamtin M, Chaisri C, Sobhon P, Setou M
  • Journal Title: PLos One Volume: Vo. 7
• [Journal Article] (2012)
  • Author(s): Goto-Inoue N, Manabe Y, Miyatake S, Ogino S, Morishita A, Hayasaka T, Masaki N, Setou M, Fujii NL
  • Journal Title: Anal Bioanal Chem Volume: (in press)
  • Peer Reviewed
• [Journal Article] Composition and Localization of Lipids in Penaeus merguiensis Ovaries during the Ovarian Maturation Cycle as Revealed by Imaging Mass Spectrometry (2012)
  • Author(s): Chansela P, Goto-Inoue N, Zaima N, Hayasaka T, Sroyraya M, Kornthong N, Engsusophon A, Tamtin M, Chaisri C, Sobhon P, Setou M
  • Journal Title: PLos One Volume: 7
  • Peer Reviewed
• [Journal Article] Visualization of dynamic change in contraction-induced lipid composition in mouse skeletal muscle by matrix-assisted laser desorption/ionization imaging mass spectrometry (2012)
  • Author(s): Goto-Inoue N, Manabe Y, Miyatake S, Ogino S, Morishita A, Hayasaka T, Masaki N, Setou M, Fujii NL
  • Journal Title: Anal Bioanal Chem Volume: (in press)
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Goto-Inoue N, Hayasaka T, Setou M
  • Journal Title: Methods Enzymol. Volume: Vo. 478 Pages: 287-301
• [Journal Article] (2011)
  • Author(s): Katagiri U Y, Sato B, Yamatoya K, Taki T, Goto-Inoue N., Setou M, Okita H, Fujimoto J, Ito T, Toshimori K, Kiyokawa N.
  • Journal Title: Biochem Biophys Res Commun Volume: Vo. 406 Pages: 326-331
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Valdes-Gonzalez, Goto-Inoue N, Hirano W, Ishiyama H, Hayasaka T, Setou M, Taki T
  • Journal Title: J Neurochem Volume: Vo. 116 Pages: 678-683
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Zaima N, Goto-Inoue N, Hayasaka T, Enomoto H, Setou M
  • Journal Title: Anal Bioanal Chem Volume: Vo. 400 Pages: 1865-1871
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Miyamura N, Nakamura T, Goto-Inoue N, S, Sakaida I, Setou M, Nishina H
  • Journal Title: Biochem Biophys Res Commun Volume: Vo. 408 Pages: 120-125
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Xu H, Kongmanas K, Kadunganattil S, Smith CE, Rupar T, Goto-Inoue N, Hermo L, Faull KF, Tanphaichitr N
  • Journal Title: J Lipid Res Volume: Vo. 52 Pages: 2187-2197
  • Peer Reviewed
• [Journal Article] Arylsulfatase A deficiency causes seminolipid accumulation and a lysosomal storage disorder in Sertoli cells (2011)
  • Author(s): Xu H, Kongmanas K, Kadunganattil S, Smith CE, Rupar T, Goto-Inoue N, Hermo L, Faull KF, Tanphaichitr N
  • Journal Title: J Lipid Res Volume: 52 Pages: 2187-2197
  • Peer Reviewed
• [Journal Article] Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver (2011)
  • Author(s): Miyamura N, Nakamura T, Goto-Inoue N, Zaima N, Hayasaka T, Yamasaki T, Terai S, Sakaida I, Setou M, Nishina H
  • Journal Title: Biochem Biophys Res Commun Volume: 408 Pages: 120-125
  • Peer Reviewed
• [Journal Article] Authenticity assessment of beef origin by principal component analysis of matrix-assisted laser desorption/ionization mass spectrometric data (2011)
  • Author(s): Zaima N, Goto-Inoue N, Hayasaka T, Enomoto H, Setou M
  • Journal Title: Anal Bioanal Chem Volume: 400 Pages: 1865-1871
  • Peer Reviewed
• [Journal Article] GalNAcβ1,3-linked paragloboside carries the epitope of a sperm maturation-related glycoprotein that is recognized by the monoclonal antibody MC121. (2011)
  • Author(s): Katagiri U Y, Sato B, Yamatoya K, Taki T, Goto-Inoue N, Setou M, Okita H, Fujimoto J, Ito T, Toshimori K, Kiyokawa N.
  • Journal Title: Biochem Biophys Res Commun. Volume: (In press)
  • Peer Reviewed
• [Journal Article] New Approach for Glyco- and Lipidomics-Molecular Scanning of Human Brain Gangliosides by TLC-Blot and MALDI-QIT-TOF MS-. (2011)
  • Author(s): Valdes-Gonzalez, Goto-Inoue N, Hirano W, Ishiyama H, Hayasaka T, Setou M, Taki T., 筆頭著者
  • Journal Title: J Neurochem. Volume: 116 Pages: 678-683
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Zaima N, Goto-Inoue N, Setou M
  • Journal Title: J Oleo Sci. Volume: Vo. 60 Pages: 93-98
  • Peer Reviewed
• [Journal Article] Selective analysis of lipids by thin-layer chromatography blot matrix-assisted laser desorption/ionization imaging mass spectrometry. (2010)
  • Author(s): Zaima N, Goto-Inoue N, Setou M.
  • Journal Title: J Oleo Sci. Volume: 60 Pages: 93-98
  • Peer Reviewed
• [Journal Article] Imaging Mass Spectrometry of Glycolipids. (2010)
  • Author(s): Goto-Inoue N, Hayasaka T, Setou M.
  • Journal Title: Methods Enzymol. Volume: 478 Pages: 287-301
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Goto-Inoue N, Hayasaka T, Taki T, Valdes Gonzalez T, Setou M
  • Journal Title: Journal of Chromatography A Volume: Vo. 1216 Pages: 7096-7101
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Goto-Inoue N, Hayasaka T, Zaima N, Setou M
  • Journal Title: Glycobiology Volume: Vo. 19 Pages: 950-957
  • Peer Reviewed
• [Journal Article] A new lipidomics approach by thin-layer chromatography-blot-matrix-assisted laser desorption/ionization imaging mass spectrometry for analyzing detailed patterns of phospholipid molecular species (2009)
  • Author(s): Naoko Goto-Inoue, Takahiro Hayasaka, Takao Taki, Tania Valdes Gonzalez, Mitsutoshi Setou
  • Journal Title: Journal of Chromatography A 1216 Pages: 7096-7101
  • Peer Reviewed
• [Journal Article] The specific localization of seminolipid molecular species on mouse testis during testicular maturation revealed by imaging mass spectrometry (2009)
  • Author(s): Naoko Goto-Inoue, Takahiro Hayasaka, Nobuhiro Zaima, Mitsutoshi Setou
  • Journal Title: Glycobiology 19 Pages: 950-957
  • Peer Reviewed
• [Presentation] イメージングマススペクトロメトリーによる皮膚臨床組織切片の解析 (2011)
  • Author(s): 井上菜穂子、内田良一、早坂孝宏、中島喜美子、佐野栄紀、瀬藤光利
  • Organizer: 第36回日本医用マススペクトル学会
  • Place of Presentation: 大阪
  • Year and Date: 2011-09-15
• [Presentation] イメージングマススペクトロメトリーによる皮膚臨床組織切片の解析 (2011)
  • Author(s): 井上菜穂子、内田良一、早坂孝宏、中島喜美子、佐野栄紀、瀬藤光利
  • Organizer: 日本医用マススペクトル学会
• [Presentation] Imaging mass spectrometry for glycomics (2010)
  • Author(s): 井上菜穂子
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] イメージングマススペクトロメトリーにおける金ナノ微粒子の応用 (2010)
  • Author(s): 井上菜穂子、早坂孝宏、柏木行康、山本真理、中許昌美、瀬藤光利
  • Organizer: 第58回質量分析総合討論会,第1回アジア・オセアニア質量分析会議
  • Place of Presentation: つくば
  • Year and Date: 2010-06-18
• [Presentation] イメージングマススペクトロメトリーにおける金ナノ微粒子の応用 (2010)
  • Author(s): 井上菜穂子、早坂孝宏、柏木行康、山本真理、中許昌美、瀬藤光利
  • Organizer: 第1回アジア・オセアニア質量分析会議
• [Presentation] Imaging mass spectrometry for glycomics (2010)
  • Author(s): 井上菜穂子
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
• [Presentation] イメージングマススペクトロメトリーによる糖脂質の局在解析 (2009)
  • Author(s): 井上菜穂子、早坂孝宏、財満信宏、瀬藤光利
  • Organizer: 日本糖質学会
• [Presentation] イメージングマススペクトロメトリーを用いたマウス精巣の脂質解析 (2009)
  • Author(s): 井上菜穂子、早坂孝宏、財満信宏、瀬藤光利
  • Organizer: 日本質量分析学会
• [Book] 質量顕微鏡法4. 7薄層クロマトグラフィー上でのイメージング(TLC-Blot-MALDIイメージング) (2009)
  • Author(s): 井上菜穂子、瀧孝雄
  • Publisher: シュプリンガー・ジャパン株式会社
• [Book] 質量顕微鏡法4.7薄層クロマトグラフィー上でのイメージング(TLC-Blot-MALD Iイメージング) (2009)
  • Author(s): 井上菜穂子、瀧孝雄
  • Total Pages: 9
  • Publisher: シュプリンガー・ジャパン株式会社