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Establishment of new lipidomics approach

Research Project

Project/Area Number 21780110
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionTokyo Metropolitan University (2011)
Hamamatsu University School of Medicine (2009-2010)

Principal Investigator

INOUE Naoko  首都大学東京, 人間健康科学研究科, 特任助教 (00509515)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords分析化学 / 脂質 / 薄層クロマトグラフィー / 質量分析 / 糖脂質 / リン脂質 / 分子種解析 / イメージング
Research Abstract

We successfully established the new lipidomics approach named "TLC-Blot-MALDI imaging" which visualizes the lipid pattern obtained from thin layer chromatography by imaging mass spectrometry which is new analytical skills.
In this study, we evaluated the validity of the technique, and also applied to the biological samples. As a result, we succeeded to detect very small amounts of lipids which hardly detected by conventional methods. Moreover, comprehensive analyses were also performed to know the lipid dynamics change with molecular species level.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (32 results)

All 2012 2011 2010 2009

All Journal Article (22 results) (of which Peer Reviewed: 20 results) Presentation (8 results) Book (2 results)

  • [Journal Article]2012

    • Author(s)
      Chansela P, Goto-Inoue N, Zaima N, Hayasaka T, Sroyraya M, Kornthong N, Engsusophon A, Tamtin M, Chaisri C, Sobhon P, Setou M
    • Journal Title

      PLos One

      Volume: Vo. 7

    • Related Report
      2011 Final Research Report
  • [Journal Article]2012

    • Author(s)
      Goto-Inoue N, Manabe Y, Miyatake S, Ogino S, Morishita A, Hayasaka T, Masaki N, Setou M, Fujii NL
    • Journal Title

      Anal Bioanal Chem

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Composition and Localization of Lipids in Penaeus merguiensis Ovaries during the Ovarian Maturation Cycle as Revealed by Imaging Mass Spectrometry2012

    • Author(s)
      Chansela P, Goto-Inoue N, Zaima N, Hayasaka T, Sroyraya M, Kornthong N, Engsusophon A, Tamtin M, Chaisri C, Sobhon P, Setou M
    • Journal Title

      PLos One

      Volume: 7

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of dynamic change in contraction-induced lipid composition in mouse skeletal muscle by matrix-assisted laser desorption/ionization imaging mass spectrometry2012

    • Author(s)
      Goto-Inoue N, Manabe Y, Miyatake S, Ogino S, Morishita A, Hayasaka T, Masaki N, Setou M, Fujii NL
    • Journal Title

      Anal Bioanal Chem

      Volume: (in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2011

    • Author(s)
      Goto-Inoue N, Hayasaka T, Setou M
    • Journal Title

      Methods Enzymol.

      Volume: Vo. 478 Pages: 287-301

    • Related Report
      2011 Final Research Report
  • [Journal Article]2011

    • Author(s)
      Katagiri U Y, Sato B, Yamatoya K, Taki T, Goto-Inoue N., Setou M, Okita H, Fujimoto J, Ito T, Toshimori K, Kiyokawa N.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: Vo. 406 Pages: 326-331

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article]2011

    • Author(s)
      Valdes-Gonzalez, Goto-Inoue N, Hirano W, Ishiyama H, Hayasaka T, Setou M, Taki T
    • Journal Title

      J Neurochem

      Volume: Vo. 116 Pages: 678-683

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article]2011

    • Author(s)
      Zaima N, Goto-Inoue N, Hayasaka T, Enomoto H, Setou M
    • Journal Title

      Anal Bioanal Chem

      Volume: Vo. 400 Pages: 1865-1871

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article]2011

    • Author(s)
      Miyamura N, Nakamura T, Goto-Inoue N, S, Sakaida I, Setou M, Nishina H
    • Journal Title

      Biochem Biophys Res Commun

      Volume: Vo. 408 Pages: 120-125

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article]2011

    • Author(s)
      Xu H, Kongmanas K, Kadunganattil S, Smith CE, Rupar T, Goto-Inoue N, Hermo L, Faull KF, Tanphaichitr N
    • Journal Title

      J Lipid Res

      Volume: Vo. 52 Pages: 2187-2197

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arylsulfatase A deficiency causes seminolipid accumulation and a lysosomal storage disorder in Sertoli cells2011

    • Author(s)
      Xu H, Kongmanas K, Kadunganattil S, Smith CE, Rupar T, Goto-Inoue N, Hermo L, Faull KF, Tanphaichitr N
    • Journal Title

      J Lipid Res

      Volume: 52 Pages: 2187-2197

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver2011

    • Author(s)
      Miyamura N, Nakamura T, Goto-Inoue N, Zaima N, Hayasaka T, Yamasaki T, Terai S, Sakaida I, Setou M, Nishina H
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 408 Pages: 120-125

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Authenticity assessment of beef origin by principal component analysis of matrix-assisted laser desorption/ionization mass spectrometric data2011

    • Author(s)
      Zaima N, Goto-Inoue N, Hayasaka T, Enomoto H, Setou M
    • Journal Title

      Anal Bioanal Chem

      Volume: 400 Pages: 1865-1871

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GalNAcβ1,3-linked paragloboside carries the epitope of a sperm maturation-related glycoprotein that is recognized by the monoclonal antibody MC121.2011

    • Author(s)
      Katagiri U Y, Sato B, Yamatoya K, Taki T, Goto-Inoue N, Setou M, Okita H, Fujimoto J, Ito T, Toshimori K, Kiyokawa N.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New Approach for Glyco- and Lipidomics-Molecular Scanning of Human Brain Gangliosides by TLC-Blot and MALDI-QIT-TOF MS-.2011

    • Author(s)
      Valdes-Gonzalez, Goto-Inoue N, Hirano W, Ishiyama H, Hayasaka T, Setou M, Taki T., 筆頭著者
    • Journal Title

      J Neurochem.

      Volume: 116 Pages: 678-683

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2010

    • Author(s)
      Zaima N, Goto-Inoue N, Setou M
    • Journal Title

      J Oleo Sci.

      Volume: Vo. 60 Pages: 93-98

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selective analysis of lipids by thin-layer chromatography blot matrix-assisted laser desorption/ionization imaging mass spectrometry.2010

    • Author(s)
      Zaima N, Goto-Inoue N, Setou M.
    • Journal Title

      J Oleo Sci.

      Volume: 60 Pages: 93-98

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Imaging Mass Spectrometry of Glycolipids.2010

    • Author(s)
      Goto-Inoue N, Hayasaka T, Setou M.
    • Journal Title

      Methods Enzymol.

      Volume: 478 Pages: 287-301

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      Goto-Inoue N, Hayasaka T, Taki T, Valdes Gonzalez T, Setou M
    • Journal Title

      Journal of Chromatography A

      Volume: Vo. 1216 Pages: 7096-7101

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      Goto-Inoue N, Hayasaka T, Zaima N, Setou M
    • Journal Title

      Glycobiology

      Volume: Vo. 19 Pages: 950-957

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new lipidomics approach by thin-layer chromatography-blot-matrix-assisted laser desorption/ionization imaging mass spectrometry for analyzing detailed patterns of phospholipid molecular species2009

    • Author(s)
      Naoko Goto-Inoue, Takahiro Hayasaka, Takao Taki, Tania Valdes Gonzalez, Mitsutoshi Setou
    • Journal Title

      Journal of Chromatography A 1216

      Pages: 7096-7101

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The specific localization of seminolipid molecular species on mouse testis during testicular maturation revealed by imaging mass spectrometry2009

    • Author(s)
      Naoko Goto-Inoue, Takahiro Hayasaka, Nobuhiro Zaima, Mitsutoshi Setou
    • Journal Title

      Glycobiology 19

      Pages: 950-957

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] イメージングマススペクトロメトリーによる皮膚臨床組織切片の解析2011

    • Author(s)
      井上菜穂子、内田良一、早坂孝宏、中島喜美子、佐野栄紀、瀬藤光利
    • Organizer
      第36回日本医用マススペクトル学会
    • Place of Presentation
      大阪
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] イメージングマススペクトロメトリーによる皮膚臨床組織切片の解析2011

    • Author(s)
      井上菜穂子、内田良一、早坂孝宏、中島喜美子、佐野栄紀、瀬藤光利
    • Organizer
      日本医用マススペクトル学会
    • Related Report
      2011 Final Research Report
  • [Presentation] Imaging mass spectrometry for glycomics2010

    • Author(s)
      井上菜穂子
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] イメージングマススペクトロメトリーにおける金ナノ微粒子の応用2010

    • Author(s)
      井上菜穂子、早坂孝宏、柏木行康、山本真理、中許昌美、瀬藤光利
    • Organizer
      第58回質量分析総合討論会,第1回アジア・オセアニア質量分析会議
    • Place of Presentation
      つくば
    • Year and Date
      2010-06-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] イメージングマススペクトロメトリーにおける金ナノ微粒子の応用2010

    • Author(s)
      井上菜穂子、早坂孝宏、柏木行康、山本真理、中許昌美、瀬藤光利
    • Organizer
      第1回アジア・オセアニア質量分析会議
    • Related Report
      2011 Final Research Report
  • [Presentation] Imaging mass spectrometry for glycomics2010

    • Author(s)
      井上菜穂子
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
    • Related Report
      2011 Final Research Report
  • [Presentation] イメージングマススペクトロメトリーによる糖脂質の局在解析2009

    • Author(s)
      井上菜穂子、早坂孝宏、財満信宏、瀬藤光利
    • Organizer
      日本糖質学会
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] イメージングマススペクトロメトリーを用いたマウス精巣の脂質解析2009

    • Author(s)
      井上菜穂子、早坂孝宏、財満信宏、瀬藤光利
    • Organizer
      日本質量分析学会
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Book] 質量顕微鏡法4. 7薄層クロマトグラフィー上でのイメージング(TLC-Blot-MALDIイメージング)2009

    • Author(s)
      井上菜穂子、瀧孝雄
    • Publisher
      シュプリンガー・ジャパン株式会社
    • Related Report
      2011 Final Research Report
  • [Book] 質量顕微鏡法4.7薄層クロマトグラフィー上でのイメージング(TLC-Blot-MALD Iイメージング)2009

    • Author(s)
      井上菜穂子、瀧孝雄
    • Total Pages
      9
    • Publisher
      シュプリンガー・ジャパン株式会社
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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