Triggering mechanism on the symplectin bioluminescence by reactive oxygen species

• Project/Area Number: 21780111
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Nagoya University
• Principal Investigator: KUSE Masaki Nagoya University, 物質科学国際研究センター, 助教 (40335013)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 生物発光 / 発光タンパク質 / 活性酸素 / 発光速度 / クロモフォア / 活性酸素種 / 発光素子 / 発光タンパク

Research Abstract

Triggering mechanism of photoproteins has been investigated. The photoproteins have chromophore which is constituted from dehydrocoelenterazine (DCL). It demonstrated that oxo-complex of Fe ion made the luminescence speed fast, on the other hand, peroxide of hypochlorite made the luminescence speed slow. This specific reaction rate was proved to be useful for the identification of reactive oxygen species by using photoproteins. A convenient synthetic method was also established to prepare many kinds of DCLs.

Research Products

• [Journal Article] Identification of a fluorescent compound in the cuticle of the train millipede Parafontaria laminata armigera. (2010)
  • Author(s): Masaki Kuse, Miho Yanagi, Eiko Tanaka, Naoki Tani, Toshio Nishikawa.
  • Journal Title: Biosci.Biotechnol.Biochem. 74 Pages: 2307-2309
  • NAID: 10027561792
• [Journal Article] Identification of a fluorescent compound in the cuticle of the train millipede Parafontaria laminata armigera (2010)
  • Author(s): Kuse, M.; Yanagi, M.; Tanaka, E.; Tani, N.; Nishikawa, T.
  • Journal Title: Biosci.Biotechnol.Biochem Volume: 74 Pages: 2307-2309
  • NAID: 10027561792
  • Peer Reviewed
• [Journal Article] Dehydrocoelenterazine is the organic substance constituting the prosthetic group of pholasin. (2009)
  • Author(s): Eiko Tanaka, Masaki Kuse, Toshio Nishikawa.
  • Journal Title: ChemBioChem 10 Pages: 2725-2729
• [Journal Article] Chemistry for biology of symplectin bioluminescence with fluoro-dehydrocoelenterazine. (2009)
  • Author(s): Yosuke Nakashima, Vorawan Kongjinda, Naoki Tani, Masaki Kuse, Minoru Isobe.
  • Journal Title: Chemiluminescence and Bioluminescence Pages: 51-54
• [Journal Article] Differentiation of coelenteramide sulfate from phosphate by hydrogen/deuterium exchange coupled with ion trap mass spectrometry. (2009)
  • Author(s): Issei Doi, Masaki Kuse, Yosuke Nakashima, Naoki Tani, Minoru Isobe.
  • Journal Title: J.Mass Spectrom.Soc.Jpn. 57 Pages: 89-95
  • NAID: 10024837397
• [Journal Article] 1H NMR Aided Elucidation of Products Derived from Photodegradation of Ethyl 3-azido-4,6-difluorobenzoate in 2,2,2-Trifluoroethanol. (2009)
  • Author(s): Magne O.Sydnes, Masaki Kuse, Issei Doi, Minoru Isobe.
  • Journal Title: Tetrahedron 65 Pages: 3863-3870
• [Journal Article] Selective oxidation by the hydroperoxide in the photoprotein, aequorin.Bioorg. (2009)
  • Author(s): Issei Doi, Masaki Kuse, Toshio Nishikawa, Minoru Isobe.
  • Journal Title: Med.Chem. 17 Pages: 3399-3404
• [Journal Article] Substituent effect of imino-O-arenesulfonates, a coupling partner in Suzuki-Miyaura Reaction for substitution of the Pyrazine Ring: A Study for the Synthesis of Coelenterazine Analogs. (2009)
  • Author(s): Makarasen, A. ; Kuse, M. ; Nishikawa, T. ; Isobe, M.
  • Journal Title: Bull.Chem.Soc.Jpn. 82 Pages: 870-878
  • NAID: 10025126416
• [Journal Article] Prevention of Copper-Induced Calcium Influx and Cell Death by Prion-Derived Peptide in Suspension-Cultured Tobacco Cells. (2009)
  • Author(s): Kagenishi, T.; Yokawa, K.; Kuse, M.; Isobe, M.; Bouteau, F.; Kawano, T.
  • Journal Title: Zeitschrift f?r Naturforschung 64 Pages: 411-417
  • NAID: 120006771476
• [Journal Article] Dehydrocoelenterazine is the organic substance constituting the prosthetic group of pholasin (2009)
  • Author(s): Tanaka, E.; Kuse, M.; Nishikawa, T.
  • Journal Title: ChemBioChem 10 Pages: 2725-2729
  • Peer Reviewed
• [Journal Article] Selective oxidation by the hydroperoxide in the photoprotein, aequorin (2009)
  • Author(s): Doi, I.; Kuse, M.; Nishikawa, T.; Isobe, M.
  • Journal Title: Bioorg. Med. Chem. 17 Pages: 3399-3404
  • Peer Reviewed
• [Journal Article] Differentiation of coelenteramide sulfate from phosphate by hydrogen/deuterium exchange coupled with ion trap mass spectrometry (2009)
  • Author(s): Doi, I.; Kuse, M.; Nakashima, Y.; Tani, N.; Isobe, M.
  • Journal Title: J.Mass Spectrom. Soc.Jpn. 57 Pages: 89-95
  • NAID: 10024837397
  • Peer Reviewed
• [Presentation] ヒカリカモメガイ発光タンパク質の発光機構に関する研究 (2011)
  • Author(s): 田中瑛子、久世雅樹、西川俊夫
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
• [Presentation] 細胞内シグナル伝達機構解明の為の生物発光基質の創出 (2010)
  • Author(s): 久世雅樹、田中瑛子、西川俊夫
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学、愛知 招待講演
  • Year and Date: 2010-09-10
• [Presentation] 細胞内シグナル伝達機構解明の為の生物発光基質の創出 (2010)
  • Author(s): 久世雅樹、田中瑛子、西川俊夫
  • Organizer: 日本植物学会 第74回大会
  • Place of Presentation: 中部大学(愛知県)
  • Year and Date: 2010-09-10
• [Presentation] Identification of a fluorescent compound in a train millipede. (2010)
  • Author(s): Masaki Kuse, Miho Yanagi, Eiko Tanaka, Toshio Nishikawa.
  • Organizer: The 9^th Joint Seminar JSPS-NRCT Core University Program.
  • Place of Presentation: Bangkok
• [Presentation] Bioluminescence of a bivalve mollusk, Pholas dactylus. (2010)
  • Author(s): Masaki Kuse, Eiko Tanaka, Toshio Nishikawa.
  • Organizer: 5^th International Conference on Cutting-Edge Organic Chemistry in Asia.
  • Place of Presentation: Taipei
• [Presentation] ヒカリカモメガイ発光タンパクの発光基質に関する研究 (2010)
  • Author(s): 田中瑛子、久世雅樹、西川俊夫
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
• [Presentation] キシャヤスデの蛍光物質に関する研究 (2010)
  • Author(s): 柳美帆、田中瑛子、谷直紀、久世雅樹、西川俊夫
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
• [Presentation] Bioluminescence of a bivalve mollusk, Pholas dactyplus (2010)
  • Author(s): Kuse, M.; Tanaka, E.; Nishikawa, T
  • Organizer: 5^<th> International Conference on Cutting-Edge Organic Chemistry in Asia
  • Place of Presentation: 台北(台湾)
• [Presentation] 二枚貝発光タンパクの発光機構に関する研究 (2009)
  • Author(s): 田中瑛子、久世雅樹、西川俊夫
  • Organizer: 日本農芸化学会第156回中部支部例会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-10-03
• [Presentation] キシャヤスデの蛍光物質に関する研究 (2009)
  • Author(s): 柳美帆、田中瑛子、谷直紀、久世雅樹、西川俊夫
  • Organizer: 日本農芸化学会第156回中部支部例会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-10-03
• [Presentation] Chemical Biology of the marine bioluminescence with coelenterazines. (2009)
  • Author(s): Masaki Kuse.
  • Organizer: The Seminar of the JSPS-NRCT Core University Program.
  • Place of Presentation: Hat Yai (Thailand)
  • Year and Date: 2009-09-08
• [Presentation] 二枚貝発光タンパク質に関する生物有機化学的研究 (2009)
  • Author(s): 久世雅樹
  • Organizer: 生物発光化学発光研究会第26回学術講演会
  • Place of Presentation: 東京 招待講演
  • Year and Date: 2009-06-06
• [Presentation] 二枚貝発光タンパク質に関する生物有機化学的研究 (2009)
  • Author(s): 久世雅樹
  • Organizer: 生物発光化学発光研究会 第26回学術講演会
  • Place of Presentation: 電気通信大学(東京)
  • Year and Date: 2009-06-06
• [Book] Chemiluminescence and Bioluminescence (2009)
  • Author(s): Nakashima, Y.; Kongjinda, V.; Tani, N.; Kuse, M.; Isobe, M.
  • Total Pages: 477
  • Publisher: World Scientific
• [Remarks] ホームページ等
  • URL: http://www.cic.nagoya-u.ac.jp/