| Project/Area Number |
21780127
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Food science
|
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| Research Institution | Kinki University (2010)
The University of Tokushima (2009) |
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Principal Investigator |
MUROTA Kaeko Kinki University, 理工学部, 講師 (40294681)
|
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| Research Collaborator |
TAKAHASHI Nobuyuki 京都大学, 大学院・農学研究科, 助教 (50370135)
WATANABE Yomi 大阪市立工業研究所, 研究員 (60416310)
STORCH Judith Rutgers University, Department of Nutritional Sciences, Professor
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 脂質 / 脂肪酸結合タンパク質 / モノグリセリド / 小腸 |
|---|
| Research Abstract |
In this study, in vitro binding assay, cell culture study, and in vivo rat study were performed. In in vitro study, the binding abilities of monoacylglycerols (MGs) and free fatty acids to intestinal fatty-acid binding proteins (FABPs) were examined. The result has indicated that the carbon chain length of free fatty acids is a key factor to determine the binding ability, whereas MGs' binding was not clear. In the cell culture study, the stable Caco-2 cell line expressing CD36 membrane protein was established. Furthermore, the lymphatic transport of various dietary lipids was estimated using thoracic-lymph cannulated rats.
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