| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
Microbicidal mechanism of carbon dioxide micro-and nano-bubbles at a lower pressure (MNB-CO_2) were investigated and Hiochi bacteria and enzymes in unpasteurized sake were inactivated by the MNB-CO_2. There were no phenomena that Sachharomyces cerevisiae cells were burst by the MNB-CO_2, although the leakage of intracellular matrix was observed. Hiochi bacteria in unpasteurized sake could not be detected and α-amylase, glucoamylase and acid carboxypeptidase be completely inactivated by the MNB-CO_2.
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