Study on enhancement of human cancer cell radiosensitivity by conjugated eicosapentaenoic acid

• Project/Area Number: 21780136
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Food science
• Research Institution: Kobe Gakuin University
• Principal Investigator: YONEZAWA Yuko (KUMAMOTO Yuko) 神戸学院大学, 栄養学部, 研究員 (80388777)
• Research Collaborator: MIZUSHINA Yoshiyuki 神戸学院大学, 栄養学部, 准教授 (20307705) ; SASAKI Ryohei 神戸大学, 大学院・医学研究科, 准教授 (30346267)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 共役エイコサペンタエン酸(cEPA) / 放射線 / がん治療 / 併用効果 / DNA合成酵素 / 酵素阻害剤 / がん細胞 / エイコサペンタエン酸(EPA) / 共役EPA(cEPA) / ヒトがん細胞 / 抗がん活性 / マイクロアレイ / 抗がん剤 / エイコサペンタエン酸 / 共役EPA / 共役脂肪酸 / アポトーシス / DNAポリメラーゼ

Research Abstract

We found that conjugated eicosapentaenoic acid(cEPA), which is a major fish oil, had anti-cancer activity based on DNA polymerase inhibition. In human colon cancer cells, the post-irradiation addition of cEPA significantly enhanced cancer cell radiosensitivity and apoptosis. cEPA also enhanced the suppression of human colon cancer cell growth by gemcitabine, which is a major clinical anti-cancer drug. cEPA could be a anti-cancer enhancer for radiotherapy and chemotherapy.

Research Products

• [Journal Article] Enhancement of human cancer cell radiosensitivity by conjugated eicosapentaenoic acid-a mammalian DNA polymerase inhibitor (2010)
  • Author(s): Y. Kumamoto-Yonezawa, R. Sasaki, Y. Suzuki, Y. Matsui, T. Hada, K. Uryu, K. Sugimura, H. Yoshida, Y. Mizushina
  • Journal Title: Int. J. Oncol Volume: vol.36, no.3 Issue: 3 Pages: 577-584
  • DOI: 10.3892/ijo_00000532
• [Journal Article] Enhancement of human cancer cell radiosensitivity by conjug ated eicosapentaenoic acid-a mammalian DNA polymerase inhibitor (2010)
  • Author(s): Yonezawa, Y.K., Sasaki, R., Suzuki, Y, Matsui, Y., Hada, T., Uryu, K., Sugimura, K., Yoshida, H., Mizushina, Y.
  • Journal Title: International Journal of Oncology 36 Pages: 577-584
  • Peer Reviewed
• [Journal Article] Cell cycle arrest triggered by conjugated eicosapentaenoic acid occurs through several mechanisms including G1 checkpoint activation by induced RPA and ATR expression (2009)
  • Author(s): Y. Kumamoto-Yonezawa, R. Sasaki, Y. Ota, Y. Suzuki, S. Fukushima, T. Hada, K. Uryu, K. Sugimura, H. Yoshida, Y. Mizushina
  • Journal Title: Biochim. Biophys. Acta Volume: vol.1790, no.5 Issue: 5 Pages: 339-346
  • DOI: 10.1016/j.bbagen.2009.02.004
• [Presentation] 口腔扁平苔癬の臨床病理学的探索 (2010)
  • Author(s): 熊本裕子、南雲清子
  • Organizer: 第59回医学検査学会
  • Place of Presentation: 神戸国際会議場・神戸国際展示場
• [Presentation] cEPAの細胞増殖抑制活性の放射線併用による効果 (2009)
  • Author(s): 米澤(熊本)裕子、佐々木良平、鈴木陽子、松井由樹、瓜生圭介、羽田尚彦、杉村和郎、水品善之、吉田弘美
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] ホウレンソウ由来糖脂質MGDG(mono-galactosyl diacyl-glycerol)と放射線照射の併用による大腸癌細胞に対する増殖抑制活性 (2009)
  • Author(s): 松井由樹、佐々木良平、鈴木陽子、米澤(熊本)裕子、羽田圭一郎、前田尚輝、水品善之、吉田弘美
  • Organizer: 日本農芸化学会西日本・中四国・関西支部合同大会(第461回講演会)
• [Presentation] ホウレンソウ糖脂質画分の癌細胞増殖抑制活性における放射線照射による併用効果の検討 (2009)
  • Author(s): 松井由樹、佐々木良平、鈴木陽子、米澤(熊本)裕子、羽田圭一郎、前田尚輝、水品善之、吉田弘美
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: 長崎
• [Presentation] cEPAの細胞増殖抑制活性の放射線併用による効果 (2009)
  • Author(s): 米澤裕子、佐々木良平、鈴木陽子、松井由樹、都築毅、仲川清隆、宮澤陽夫、杉村和郎、水品善之、吉田弘美
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Book] Enhancement of human cancer cell radiosensitivity by conjugated eicosapentaenoic acid, an inhibitor of DNA polymerase and topoisomerase (ISBN : 978-1-61942-089-2) (2012)
  • Author(s): Y.Mizushina, Y.K.Yonezawa, H.Yoshida
  • Total Pages: 54
  • Publisher: Nova Science Publishers【https://www.novapublishers.com/catalog/product_info.php?products_id=31185】
• [Book] Enhancement of human cancer cell radiosensitivity by conjugated eicosapentaenoic acid, an inhibitor of DNA polymerase and topoisomerase (2011)
  • Author(s): Y. Mizushina, Y. Kumamoto-Yonezawa, H. Yoshida
  • Publisher: Nova Science Publishers
  • URL: https://www.novapublishers.com/catalog/product_info.php?products_id=31185
• [Book] Anti-Cancer Agents : New Research (Enhancement of human cancer cell radiosensitivity by conjugated eicosapentaenoic acid, an inhibitor of DNA polymerase and topoisomerase) (2011)
  • Author(s): Y.Mizushina, Y.K.Yonezawa, H.Yoshida
  • Publisher: Nova Science Publishers(in press)