Molecular biological study on the pathogenic nematode-host plant interactions at the early stage of pine wilt disease

• Project/Area Number: 21780147
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Forest science
• Research Institution: Kyoto University
• Principal Investigator: TAKEUCHI Yuko 京都大学, 大学院・農学研究科, 助教 (80452283)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 遺伝子発現 / 森林病害 / 線虫 / 相互認識機構 / プロテオーム / マツノザイセンチュウ

Research Abstract

Pine wilt disease is an infectious forest disease caused by the pathogenic pine wood nematode, Bursaphelenchus xylophilus. To elucidate the pathogenic mechanism of the disease, the interaction between the nematode and host pine at an early stage was investigated, by means of molecular biological techniques. The proteomic analyses determined the profile of surface-coat proteins and secreted proteins of the nematode, and cDNA subtraction analysis determined several genes involved in the host responses. Results obtained indicate that the pine wood nematode has a kind of system to defeat host defense responses.

Research Products

• [Journal Article] Comparison of the surface coat proteins of the pine wood nematode appeared duri host pine infection and in vitro culture by a proteomic approach (2010)
  • Author(s): Shinya, R., Morisaka, H., Takeuchi, Y., Ueda, M., and Futai, K
  • Journal Title: Phytopathology Volume: Vol.100 Issue: 12 Pages: 1289-1297
  • DOI: 10.1094/phyto-04-10-0109
  • Peer Reviewed
• [Journal Article] Comparison of the surface coat proteins of the pine wood nematode appeared during host pine infection and in vitro culture by a proteomic approach (2010)
  • Author(s): Ryoji Shinya, Hironobu Morisaka, Yuko Takeuchi, Mitsuyoshi Ueda, Kazuyoshi Futai
  • Journal Title: Phytopathology Volume: 100 Pages: 1289-1297
  • Peer Reviewed
• [Journal Article] Diagnosis and quantification of the pine wood nematode, Bursaphelenchus xylophilus(Steiner and Buhrer), in wood of Pinus thunbergii with real-time PCR (2009)
  • Author(s): Takeuchi, Y., and Futai, K
  • Journal Title: Nematological Research Volume: Vol.39 Pages: 9-16
  • URL: https://www.jstage.jst.go.jp/article/jjn/39/1/39_1_9/_pdf
  • Peer Reviewed
• [Journal Article] Surface coat proteins of the pine wood nematode, Bursaphelenchus xylophilus (2009)
  • Author(s): Shinya, R., Takeuchi, Y., Miura, N., Ueda, M., and Futai, K
  • Journal Title: profiles of stage-and isolate-specific characters Volume: Vol.11 Issue: 3 Pages: 429-438
  • DOI: 10.1163/156854109x447006
  • Peer Reviewed
• [Journal Article] Surface coat proteins of the pine wood nematode, Bursaphelenchus xylophilus : profiles of stage and isolate specific characters (2009)
  • Author(s): Ryoji Shinya, Yuko Takeuchi, Natsuko Miura, Koichi Kuroda, Mitsuyoshi Ueda, Kazuyoshi Futai
  • Journal Title: Nematology Volume: 11 Pages: 429-438
  • Peer Reviewed
• [Journal Article] Diagnosis and quantification of the pine wood nematode, Bursaphelenchus xylophilus (Steiner and Buhrer), in wood of Pinus thunbergii with real-time PCR (2009)
  • Author(s): Yuko Takeuchi, Kazuyoshi Futai
  • Journal Title: Nematological Research Volume: 39 Pages: 9-16
  • Peer Reviewed
• [Presentation] マツノザイセンチュウ接種により誘導されるクロマツの遺伝子発現プロファイル (2012)
  • Author(s): 津川和典・竹内祐子・二井一禎
  • Organizer: 第123回日本森林学会大会
  • Place of Presentation: 宇都宮大学(栃木県)
  • Year and Date: 2012-03-28
• [Presentation] プロテオーム解析から見えてきたマツノザイセンチュウの寄生戦略 (2012)
  • Author(s): 新屋良治・森坂裕信・竹内祐子・菊地泰生・植田充美・二井一禎
  • Organizer: 第123回日本森林学会大会
  • Place of Presentation: 宇都宮大学(栃木県)(招待講演)
  • Year and Date: 2012-03-28
• [Presentation] プロテオーム解析から見えてきたマツノザイセンチュウの寄生戦略 (2012)
  • Author(s): 新屋良治・森坂裕信・竹内祐子・菊地泰生・植田充美・二井一禎
  • Organizer: 第123回日本森林学会大会
  • Place of Presentation: 宇都宮大学(栃木県)
  • Year and Date: 2012-02-28
• [Presentation] Pathogenicity of the pine wood nematode, Bursaphelenchus xylophilus, revealed by proteomic analysise, Bursaphelenchus xylophilus, revealed by proteomic analysis (2011)
  • Author(s): Futai, K., Shinya, R., Takeuchi, Y., and Ueda, M
  • Organizer: 3rd International Symposium on Frontiers in Agriculture Proteome Research
  • Place of Presentation: Tsukuba International Congress Center(Ibaraki Prefecture)
  • Year and Date: 2011-11-10
• [Presentation] Pathogenicity of the pine wood nematode, Bursaphelenchus xylophilus, revealed by proteomic analysis (2011)
  • Author(s): Futai, K., Shinya, R., Takeuchi, Y., Ueda, M.
  • Organizer: 3rd International Symposium on Frontiers in Agriculture Proteome Research
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2011-11-10
• [Presentation] マツノザイセンチュウ純系間における遺伝子発現プロファイルの比較 (2011)
  • Author(s): 金子彰・新屋良治・竹本周平・竹内祐子・二井一禎
  • Organizer: 日本線虫学会第19回大会
  • Place of Presentation: 京都市国際交流会館(京都府)
  • Year and Date: 2011-09-15
• [Presentation] Surface coat and secretion proteins of the pine wood nematode : the strategy requirements for attack and defense at the host-parasite interface (2011)
  • Author(s): Shinya, R., Takeuchi, Y., Morisaka, H., Ueda, M., and Futai, K
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo Convention Center(Hokkaido)
  • Year and Date: 2011-09-09
• [Presentation] Surface coat and secretion proteins of the pine wood nematode : the strategy requirements for attack and defense at the host-parasite interface (2011)
  • Author(s): Shinya, R., Takeuchi, Y., Morisaka, H., Ueda, M., Futai, K.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションホール(北海道)
  • Year and Date: 2011-09-09
• [Presentation] A proteomic dissection of the parasitic strategy of the pinewood nematode (2011)
  • Author(s): Shinya, R., Takeuchi, Y., Morisaka, H., Kikuchi, T., Ueda, M., and Futai, K.
  • Organizer: Society of Nematologists 50th Anniversary Meeting
  • Place of Presentation: Oregon State University(USA)
  • Year and Date: 2011-07-18
• [Presentation] A proteomic dissection of the parasitic strategy of the pinewood nematode (2011)
  • Author(s): Shinya, R., Takeuchi, Y., Morisaka, H., Kikuchi, T., Ueda, M., Futai, K.
  • Organizer: Society of Nematologists 50th Anniversary Meeting
  • Place of Presentation: オレゴン州立大学(USA)
  • Year and Date: 2011-07-18
• [Presentation] Establishment of the genetically uniformed pure-lines of the pine wood nematode, Bursaphelenchus xylophilus (2010)
  • Author(s): Takeuchi, Y., Shinya, R., Ichimura, K., Takemoto, S., and Futai, K
  • Organizer: 30th International Symposium of the European Society of Nematologists
  • Place of Presentation: Vienna(Austria)
  • Year and Date: 2010-09-23
• [Presentation] Establishment of the genetically uniformed pure-lines of the pine wod nematode, Bursaphelenchus xylophilus (2010)
  • Author(s): Yuko Takeuchi, Ryoji Shinya, Keita Ichimura, Shuhei Takemoto, Kazuyoshi Futai
  • Organizer: 30th International Symposium of the European Society of Nematologists
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2010-09-23
• [Presentation] Dramatic alteration of the surface coat proteins of the pine wood nematode during host pine infection revealed by a proteomic approach (2010)
  • Author(s): Shinya, R., Morisaka, H., Takeuchi, Y., Ueda, M., and Futai, K
  • Organizer: 30th International Symposium of the European Society of Nematologists
  • Place of Presentation: Vienna(Austria)
  • Year and Date: 2010-09-22
• [Presentation] Dramatic alteration of the surface coat proteins of the pine wood nematode during host pine infection revealed by a proteomic approach (2010)
  • Author(s): Ryoji Shinya, Hironobu Morisaka, Yuko Takeuchi, Mitsuyoshi Ueda, Kazuyoshi Futai
  • Organizer: 30th International Symposium of the European Society of Nematologists
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2010-09-22
• [Presentation] 病原力の異なるマツノザイセンチュウ4系統間での比較セクレトーム解析 (2010)
  • Author(s): 新屋良治・森坂裕信・竹内祐子・植田充美・二井一禎
  • Organizer: 日本線虫学会第18回大会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2010-08-26
• [Presentation] 病原力の異なるマツノザイセンチュウ4系統間での比較セクレトーム解析 (2010)
  • Author(s): 新屋良治・森坂裕信・竹内祐子・植田充美・二井一禎
  • Organizer: 日本線虫学会第18回大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-08-26
• [Presentation] マツ感染時におけるマツノザイセンチュウ表面タンパク質のフォーカスドプロテオーム解析 (2009)
  • Author(s): 新屋良治・森坂裕信・竹内祐子・植田充美・二井一禎
  • Organizer: 日本線虫学会第17回大会
  • Place of Presentation: 崇城大学市民ホール(熊本県)
  • Year and Date: 2009-09-04
• [Presentation] マツ感染時におけるマツノザイセンチュウ表面タンパク質のフォーカスドプロテオーム解析 (2009)
  • Author(s): 新屋良治・森坂裕信・竹内祐子・植田充美・二井一禎
  • Organizer: 第17回日本線虫学会大会
  • Place of Presentation: 熊本・崇城大学市民ホール
  • Year and Date: 2009-09-04
• [Presentation] Surface coat proteins of the pine wood nematode, Bursaphelenchus xylophilus (2009)
  • Author(s): Shinya, R., Takeuchi, Y., Morisaka, H., Ueda, M., and Futai, K
  • Organizer: IUFRO 2009 International Symposium on Pine Wilt Disease
  • Place of Presentation: 南京林業大学(中国)
  • Year and Date: 2009-07-22
• [Presentation] Surface coat proteins of the pine wood nematode, Bursaphelenchus xylophilus (2009)
  • Author(s): Ryoji Shinya, Yuko Takeuchi, Hironobu Morisaka, Mitsuyoshi Ueda, Kazuyoshi Futai
  • Organizer: IUFRO 2009 International Symposium on Pine Wilt Disease
  • Place of Presentation: 中国・南京林業大学
  • Year and Date: 2009-07-22
• [Book] 微生物生態学への招待-森をめぐるミクロな世界 (2012)
  • Author(s): 二井一禎・竹内祐子・山崎理正
  • Total Pages: 353
  • Publisher: 京都大学学術出版会