| Project/Area Number |
21780153
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Forest science
|
|---|
| Research Institution | Kagawa University |
|---|
Principal Investigator |
FUKUMOTO Takeshi Kagawa University, 農学部, 産学官連携研究員 (70467835)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
|---|
| Keywords | 樹木 / プロトプラスト / プロモーター評価 |
|---|
| Research Abstract |
The transient expression of GFP gene, which regulated by known constitutive promoter in plant, was examined with protoplasts in order to compare the promoter activity in each of protoplast derived from several plant tissues. It was aimed to clarify the efficient promoter in woody plant transformation. For the electroporation study, isolation condition of protoplast was newly clarified in four kinds of mangrove including Avicennia and Sonneratia genus in addition to the previously clarified seven plants. The protoplast of Larix (conifer) showed strong GFP expression by modified 35S (El2Ω) and maize ubiquitin promoter, but not 35S promoter. El2Ω promoter were also available for Bruguiera (mangrove). To compare the promoter activity at the mRNA level, qRT-PCR-based evaluation system was developed, and the plasmid vector for bio-imaging of woody plant was constructed with EL2Ω promoter.
|
