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Molecular biological analysis of microbial community in decayed wood

Research Project

Project/Area Number 21780164
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Wood science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

YOSHIDA Makoto  Tokyo University of Agriculture and Technology, 大学院・農学研究院, 特任准教授 (30447510)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords木材腐朽 / 菌類
Research Abstract

Detection method of fungi existing in decayed wood was developed in the present study. Fungal community in decayed wood was analyzed by denaturing gradient gel electrophoresis following amplification of fungal ITS region by PCR. The analysis suggested the existence of various fungi in decayed wood. Moreover, visualization of mycelium by FISH was examined. As a result, FISH with DNA probe targeting ITS region of fungi was failed, while the analysis with PNA probe successfully visualized the mycelia of wood rotting fungi.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (12 results)

All 2011 2010 2009 Other

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] 変性剤濃度勾配ゲル電気泳動法を利用した腐朽木材中に存在する真菌類の菌叢解析2010

    • Author(s)
      中田裕治, 久住朝子, 半智史, 片山葉子, 船田良, 福田清春, 吉田誠
    • Journal Title

      木材保存 36巻,3号

      Pages: 100-110

    • NAID

      10029100551

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 変性剤濃度勾配ゲル電気泳動法を用いた木材腐朽菌の検出法の最適化2010

    • Author(s)
      中田裕治, 吉田誠, 船田良, 福田清春
    • Journal Title

      木材保存協会第26回年次大会論文集

      Pages: 46-47

    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Journal Article] 変性剤濃度勾配ゲル電気泳動法を利用した腐朽木材中に存在する真菌類の菌叢解析2010

    • Author(s)
      中田裕治, 久住朝子, 半智史, 片山葉子, 船田良, 福田清春, 吉田誠
    • Journal Title

      木材保存

      Volume: 36巻 Pages: 100-110

    • NAID

      10029100551

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 変性濃度勾配ゲル電気泳動法による木材腐朽菌の同定2009

    • Author(s)
      中田裕治, 吉田誠, 福田清春
    • Journal Title

      日本木材保存協会第25回年次大会論文集

      Pages: 48-49

    • Related Report
      2009 Annual Research Report
  • [Journal Article] 変性剤濃度勾配ゲル電気泳動法を利用した腐朽木材中に存在する真菌類の菌叢解析

    • Author(s)
      中田裕治, 久住朝子, 半智史, 片山葉子, 船田良, 福田清春, 吉田誠
    • Journal Title

      木材保存 (In press)

    • NAID

      10029100551

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 変性剤濃度勾配ゲル電気泳動法を用いた木材腐朽菌の検出法の最適化2011

    • Author(s)
      中田裕治, 吉田誠, 船田良, 福田清春
    • Organizer
      日本木材保存協会第26回年次大会
    • Place of Presentation
      メルパルク東京(東京都)
    • Year and Date
      2011-05-25
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] サクラの樹幹における真菌類の局在様式の調査2011

    • Author(s)
      中田裕治, 永石憲道, 福田清春, 吉田誠
    • Organizer
      第61回日本木材学会大会
    • Place of Presentation
      京都大学(京都)
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 糖質加水分解酵素ファミリー7に属する酵素遺伝子の腐朽材からの検出2011

    • Author(s)
      堀友宣, 中田裕治, 福田清春, 吉田誠
    • Organizer
      第61回日本木材学会大会
    • Place of Presentation
      京都大学(京都)
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 変性剤濃度勾配ゲル電気泳動法(DGGE法)を用いた腐朽材の菌叢解析2010

    • Author(s)
      中田裕治, 福田清春, 吉田誠
    • Organizer
      第60回日本木材学会大会
    • Place of Presentation
      宮崎観光ホテル(宮崎県)
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] 変性剤濃度勾配ゲル電気泳動法を用いた木材腐朽に関わる糸状菌群の解析2009

    • Author(s)
      中田裕治, 久住朝子, 片山葉子, 吉田誠, 福田清春
    • Organizer
      第9回糸状菌分子生物学コンファレンス
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2009-11-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] 変性剤濃度勾配ゲル電気泳動法による木材腐朽菌の同定2009

    • Author(s)
      中田裕治, 吉田誠, 福田清春
    • Organizer
      日本木材保存協会第25回年次大会
    • Place of Presentation
      メルパルク東京(東京都)
    • Year and Date
      2009-05-21
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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