| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
The sex differentiation in crustaceans is known to be controlled by a peptide hormone called androgenic gland hormone (AGH). The primary structure of the terrestrial isopod Armadillidium vulgare AGH (Arv-AGH) was finally determined to be a heterodimeric glycoprotein. An N-linked glycan moiety in the mature Arv-AGH was found to be essential for biological activity. Therefore, in this study, a recombinant AGH with a glycan moiety was produced using a baculovirus expression system. Insect Sf9 cells were infected with a recombinant baculovirus expression vector containing an Arv-AGH cDNA insert and subsequently recombinant Arv-AGH was expressed. In Western blot analysis using an anti-Arv-AGH antibody, immunoreactive band of a glycosylated recombinant Arv-AGH was detected.
Finally, a cDNA encoding AGH-like peptide (Mar-IAG) was cloned from the giant freshwater prawn Macrobrachium rosenbergii and subsequently a recombinant Mar-IAG with a glycan moiety was expressed by the same methods as recombinant Arv-AGH.
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