Genetic diversity and phylogenetic relationship of ciliates and its algal prey ingested by toxic dinoflagellate Dinophysis spp.

• Project/Area Number: 21780191
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General fisheries
• Research Institution: Tohoku University (2010-2011); Fisheries Research Agency (2009)
• Principal Investigator: NISHITANI Goh 東北大学, 大学院・農学研究科, 助教 (70450781)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 下痢性貝毒 / プランクトン / 生態 / Dinophysis / 分子生態 / 餌料生物 / Myrionecta rubra / クリプト藻

Research Abstract

This study showed the ecological relationship among Dinophysis, ciliate, and cryptophyte in Japanese coastal areas. Many Dinophysis species ingested the ciliate Myrionecta rubra, and further the ciliate preferentially ingested the cryptophyte Teleaulax amphioxeia. We also revealed that Dinophysis mitra retained more than 100 kinds of plastids robbed from algal prey.

Research Products

• [Journal Article] Multiple plastids collected by the dinoflagellate Dinophysis mitra through kleptoplastidy (2012)
  • Author(s): Nishitani G, Nagai S, Hayakawa S, Kosaka Y, Sakurada K, Kamiyama T, Gojyobori T.
  • Journal Title: Applied and Environmental Microbiology Volume: 78 Pages: 813-821
  • Peer Reviewed
• [Journal Article] Direct detection of harmful algae from the oyster spat and live fish transporting trailers (2010)
  • Author(s): Matsuyama Y, Nishitani G, Nagai S.
  • Journal Title: Proceedings of 13th International Conference on Harmful Algae Pages: 185-189
  • Peer Reviewed
• [Journal Article] Genetic analyses of the cryptomonad nucleomorph in natural Myrionecta rubra cells reveal a simple lineage of Dinophysis kleptoplastids (2010)
  • Author(s): Nishitani G, Nagai S, Baba K, Kiyokawa S, Kosaka Y, Miyamura K, Nishikawa T, Sakurada K, Shinada A, Kamiyama T.
  • Journal Title: Proceedings of 13th International Conference on Harmful Algae Pages: 175-159
  • Peer Reviewed
• [Journal Article] Genetic structuring and gene flow of marine dinoflagellate Cochlodinium polykrikoides in Japanese and Korean coastal waters revealed by microsatellites (2010)
  • Author(s): Nagai S, Nishitani G, Sakamoto S, Sugaya T, Lee CK, Kim CH, Itakura S, Yamaguchi M.
  • Journal Title: Proceedings of 13th International Conference on Harmful Algae Pages: 171-174
  • Peer Reviewed
• [Journal Article] Genetic structure of Asian geographic populations in Alexandrium catenella (Dinophyceae): a study using microsatellite markers (2010)
  • Author(s): Genovesi B, Nishitani G, Wang J, Masseret E, Grzebyk D, Berrebi P, Nagai S.
  • Journal Title: Proceedings of 13th International Conference on Harmful Algae Pages: 161-165
  • Peer Reviewed
• [Journal Article] High-level congruence of Myrionecta rubra prey and Dinophysis species plastid identities as revealed by genetic analyses of isolates from Japanese coastal waters (2010)
  • Author(s): Nishitani G, Nagai S, Baba K, Kiyokawa S, Kosaka Y, Miyamura K, Nishikawa T, Sakurada K, Shinada A, Kamiyama T
  • Journal Title: Applied and Environmental Microbiology 76, 2791-2798. 査読有 Volume: 76 Pages: 2791-2798
  • Peer Reviewed
• [Journal Article] Genetic analyses of the cryptomonad nucleomorph in natural Myrionecta rubra cells reveal a simple lineage of Dinophysis kleptoplastids. (2010)
  • Author(s): Goh Nishitani
  • Journal Title: Proceedings of 13th International Conference on Harmful Algae Pages: 175-179
  • Peer Reviewed
• [Journal Article] High-level congruence of Myrionecta rubra prey and Dinophysis species plastididentities as revealed by genetic analses of isolates from Japanese coastal waters. (2010)
  • Author(s): Goh Nishitani
  • Journal Title: Applied and Environmental Microbiology 76 Pages: 2791-2798
  • Peer Reviewed
• [Presentation] 気仙沼・舞根湾における生物環境調査-3, プランクトンの現況 (2012)
  • Author(s): 山田雄一郎, 西谷豪, 気仙沼
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
• [Presentation] 女川湾に出現する多毛類浮遊幼生の同定と季節変動 (2012)
  • Author(s): 阿部博和, 西谷豪
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
• [Presentation] オキアミ類に付着する繊毛虫の分類学的研究 (2012)
  • Author(s): 遠藤宜成, 西谷豪
  • Organizer: 2012年度日本海洋学会春季大会
  • Place of Presentation: 茨城県つくば市
• [Presentation] 気仙沼・舞根湾における生物環境調査-3プランクトンの現況 (2012)
  • Author(s): 山田雄一郎、西谷豪
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
• [Presentation] オキアミ類に付着する繊毛虫の分類学的研究 (2012)
  • Author(s): 遠藤宜成、西谷豪, 他2名
  • Organizer: 2012年度日本海洋学会春季大会
  • Place of Presentation: 筑波大学
• [Presentation] 海洋プランクト (2011)
  • Author(s): 西谷豪
  • Organizer: 森は海の恋人シンポジウム「海と共に生きる」
  • Place of Presentation: 仙台市民会館
  • Year and Date: 2011-12-21
• [Presentation] 海洋プランクトン (2011)
  • Author(s): 西谷豪、山田雄一郎
  • Organizer: 森は海の恋人シンポジウム「海と共に生きる」
  • Place of Presentation: 仙台市民会館(招待講演)
  • Year and Date: 2011-12-21
• [Presentation] 海産繊毛虫Spirotontonia grandisから検出された葉緑体配列の遺伝的多様性 (2011)
  • Author(s): 西谷豪
  • Organizer: 日本藻類学会第35回大会
  • Place of Presentation: 富山県富山市
  • Year and Date: 2011-03-27
• [Presentation] 海産繊毛虫Spirotontonia grandisから検出された葉緑体配列の遺伝的多様性 (2011)
  • Author(s): 西谷豪
  • Organizer: 日本藻類学会第35回大会
  • Place of Presentation: 富山大学
  • Year and Date: 2011-03-27
• [Presentation] 形態及び分子生物学的手法を用いた多毛類浮遊幼生の同定の試み (2011)
  • Author(s): 阿部博和, 西谷豪
  • Organizer: 2011年度日本ベントス学会・日本プランクトン学会合同大会
  • Place of Presentation: 高知大学
• [Presentation] Morphology, feeding characteristics and taxonomy of a novel diatom-feeding flagellate isolated from bottom sediment of Onagawa Bay, northeastern Japan (2011)
  • Author(s): H.Ohno, G.Nishitani, 他2名
  • Organizer: 1st Asian Congress of Protistology 8th Asian Conference on Ciliate Biology
  • Place of Presentation: Jeju, Korea
• [Presentation] 有毒渦鞭毛藻Dinophysis triposの増殖特性と毒生産 (2010)
  • Author(s): 神山孝史, 西谷豪
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都府京都市
• [Presentation] 渦鞭毛藻Dinophysis mitra 1細胞から検出した葉緑体遺伝子配列の多様性 (2009)
  • Author(s): 西谷豪
  • Organizer: 平成21年度日本水産学会秋季大会
  • Place of Presentation: 岩手県盛岡市
• [Presentation] 夏季石巻湾における下痢性貝毒原因種Dinophysis属と繊毛虫類の出現変動の関係 (2009)
  • Author(s): 神山孝史, 西谷豪
  • Organizer: 平成21年度日本水産学会秋季大会
  • Place of Presentation: 岩手県盛岡市