The molecular mechanism of catch contraction based on the structural characteristics of twitchin of bivalve smooth muscle

• Project/Area Number: 21780195
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Fisheries chemistry
• Research Institution: Mie University
• Principal Investigator: FUNABARA Daisuke Mie University, 大学院・生物資源学研究科, 准教授 (00335150)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: キャッチ収縮 / 二枚貝 / ミオシン / アクチン / トゥイッチン / リン酸化 / 等温滴定カロリメトリー / RNA干渉

Research Abstract

Myosin S1 of catch muscle of mussel Mytilus galloprovincialis was expressed using a protein expression system using the wheat germ extract. The myosin binding regions on the twitchin molecule were predicted with the ITC analysis using the peptides designed according to the sequence of twitchin and the myosin loop 2 peptide. The expression of myosin and paramyosin genes in catch muscles of mussel was suppressed by the RNA interference methods.

Research Products

• [Journal Article] 二枚貝キャッチ運動の分子メカニズム (2009)
  • Author(s): 舩原大輔
  • Journal Title: 溶接学会誌 78 Pages: 25-29
  • NAID: 10025074379
• [Journal Article] Myosin loop 2 is involved in the formation of a trimeric complex of twitchin, actin and myosin. (2009)
  • Author(s): Daisuke Funabara, Rika Osawa, Miki Ueda, Satoshi Kanoh, David J.Hartshorne,Shugo Watabe.
  • Journal Title: J.Biol.Chem. 284 Pages: 18015-18020
  • Peer Reviewed
• [Journal Article] Myosin loop 2 is involved in the formation of a trimeric complex of twitehin, actin and myosin. (2009)
  • Author(s): Daisuke Funabara, Rika Osawa, Miki Ueda, Satoshi Kanoh, David J. Hartshorne, Shugo Watabe
  • Journal Title: The Journal of Biological Chemistry 284 Pages: 18015-18020
  • Peer Reviewed
• [Presentation] RNA干渉法によるキャッチ筋タンパク質ノックダウン二枚貝作出の検討 (2010)
  • Author(s): 長尾知樹・六分一早希・舩原大輔・加納哲
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都大学(京都府京都市)
  • Year and Date: 2010-09-24
• [Presentation] RNA干渉法によるキャッチ筋タンパク質ノックダウン二枚貝作出の検討 (2010)
  • Author(s): 長尾知樹・六分一早希・舩原大輔・加納哲
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2010-09-24
• [Presentation] キャッチ筋トゥイッチンの結合によるF-アクチンの構造変化 (2010)
  • Author(s): 横谷沙季・舩原大輔・渡部終五・加納哲
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 日本大学(神奈川県藤沢市)
  • Year and Date: 2010-03-27
• [Presentation] キャッチ筋トゥイッチンの結合によるF-アクチンの構造変化 (2010)
  • Author(s): 横谷沙季, 舩原大輔, 渡部終五, 加納哲
  • Organizer: 日本水産学会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2010-03-27
• [Presentation]
  • Author(s): .