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Establishment of the method of selecting in vitro matured oocyte with high developmental competence by morphological evaluation of metaphase spindle.

Research Project

Project/Area Number 21780250
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied animal science
Research InstitutionTohoku University

Principal Investigator

HOSHINO Yumi  東北大学, 大学院・農学研究科, 助教 (10451551)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsマウス / 卵子 / 体外成熟培養 / 卵子の質評価 / 受精能・発生能 / 紡錘体 / 卵子の質 / MTOC / 微小管の安定性 / 受精能・発生能評価 / Akt
Research Abstract

It is important to identify reliable indicators of oocyte quality for efficient embryo production and infertility treatment. Determination of oocyte quality by morphological assessment is a relatively popular method because it is noninvasive and convenient. In this study, the feature of the high quality oocyte was clarified based on the meiotic spindle of a mature oocyte. Furthermore, the factor in connection with this was identified and the index which leads to evaluation of the quality of an oocyte was established based on the result in a molecular level.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (34 results)

All 2013 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 8 results) Presentation (17 results) (of which Invited: 2 results) Book (2 results) Remarks (5 results)

  • [Journal Article] Distribution and association of mTOR with its cofactors2013

    • Author(s)
      Yuhei KOGASAKA, Yumi HOSHINO, Yuki HIRADATE, Kentaro TANEMURA, Eimei SATO
    • Journal Title

      raptor and rictor, in cumulus cells and oocytes during meiotic maturation in mice, Molecular Reproduction and Development

      Volume: Vol.80, No.4 Issue: 4 Pages: 334-348

    • DOI

      10.1002/mrd.22166

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of maturation competence of metaphaseII oocytes in mice based on thedistance between pericentriolar materials of meiotic spindle2011

    • Author(s)
      Chizuka SAKAI, Yumi HOSHINO, Yusuke SATO, Eimei SATO
    • Journal Title

      Journalof Assisted Reproductionand Genetics

      Volume: Vol.28, No.1 Issue: 2 Pages: 157-166

    • DOI

      10.1007/s10815-010-9496-2

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of different culture conditions on mouse oocyte quality in in vitro maturation: droplet size and the number of oocyte examined2011

    • Author(s)
      Manami NISHIO, Yumi HOSHINO, Eimei SATO
    • Journal Title

      Journal of Mammalian Ova Research

      Volume: Vol. 28, No.1 Pages: 53-60

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] IVM卵子の成熟機構2011

    • Author(s)
      星野由美、佐藤優介、佐藤英明
    • Journal Title

      産婦人科の実際

      Volume: 60 Pages: 1361-1365

    • NAID

      40019002000

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 加齢による卵子への影響-体外培養卵子の細胞質成熟2011

    • Author(s)
      星野由美
    • Journal Title

      産科と婦人科

      Volume: 78 Pages: 923-928

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Evaluation of maturation competence of metaphase II oocytes in mice based on the distance between pericentriolar materials of meiotic spindle2011

    • Author(s)
      Chizuka Sakai, Yumi Hoshino, Yusuke Sato, Eimei Sato
    • Journal Title

      Journal of Assisted Reproduction and Genetics

      Volume: 28 Pages: 157-166

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of different culture conditions on mouse oocyte quality in in vitro maturation : droplet size and the number of oocyte examined2011

    • Author(s)
      Manami Nishio, Yumi Hoshino, Eimei Sato
    • Journal Title

      Journal of Mammalian Ova Research

      Volume: 28 Pages: 53-60

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The signal transduction of meiotic progression in mammalian oocyte2010

    • Author(s)
      Yumi HOSHINO, Yusuke SATO, Chizuka SAKAI, Eimei SATO
    • Journal Title

      Journal of Mammalina Ova Research

      Volume: Vol.27, No.1 Pages: 21-26

    • NAID

      10026350391

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 卵成熟における紡錘体形成と染色体分配の分子メカニズム2010

    • Author(s)
      星野由美、佐藤優介、坂井知津香、佐藤英明
    • Journal Title

      Journal of Clinical Embryologist

      Volume: 12巻 Pages: 1-7

    • URL

      http://embryologist.jp/special/?id=6356

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The signal transduction of meiotic progression in mammalian oocyte2010

    • Author(s)
      星野由美、佐藤優介、坂井知津香、佐藤英明
    • Journal Title

      Journal of Mammalina Ova Research 27(印刷中)

    • NAID

      10026350391

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 卵成熟機構からみたARTに応用できる基礎研究2013

    • Author(s)
      星野由美
    • Organizer
      第31回日本受精着床学会総会・学術講演会
    • Place of Presentation
      大分
    • Year and Date
      2013-08-08
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス卵丘細胞・卵母細胞におけるmTORおよびその複合体構成因子raptor,rictorの局在解析2013

    • Author(s)
      小賀坂祐平、星野由美、種村健太郎、佐藤英明
    • Organizer
      第54回日本卵子学会
    • Place of Presentation
      東京
    • Year and Date
      2013-05-25
    • Related Report
      2011 Final Research Report
  • [Presentation] 卵成熟機構からみたARTに応用できる基礎研究2013

    • Author(s)
      星野由美
    • Organizer
      第31回日本受精着床学会・学術講演会
    • Place of Presentation
      大分
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] 微小管形成能力による成熟卵子のクオリティー評価2011

    • Author(s)
      星野由美、坂井知津香、佐藤英明
    • Organizer
      第104回日本繁殖生物学会
    • Place of Presentation
      岩手
    • Year and Date
      2011-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] 卵子の成熟と胚の品質評価:卵成熟メカニズムに基づいた成熟卵子のクオリティー評価2011

    • Author(s)
      星野由美
    • Organizer
      第29回日本受精着床学会総会・学術講演会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-09
    • Related Report
      2011 Final Research Report
  • [Presentation] 発生能の高い成熟卵子獲得を目指した紡錘体形成メカニズムの解明2011

    • Author(s)
      坂井知津香、星野由美、佐藤英明
    • Organizer
      第6回日本生殖再生医学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2011-03-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] 卵子の成熟と胚の品質評価: 卵成熟メカニズムに基づいた成熟卵子のクオリティー評価2011

    • Author(s)
      星野由美
    • Organizer
      第29回日本受精着床学会総会・学術講演会
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] 微小管形成能力による成熟卵子のクオリティー評価2011

    • Author(s)
      星野由美
    • Organizer
      第104回日本繁殖生物学会大会
    • Place of Presentation
      岩手
    • Related Report
      2011 Annual Research Report
  • [Presentation] マウス卵丘細胞・卵母細胞におけるmTORおよびその複合体構成因子raptor、rictorの局在解析2011

    • Author(s)
      小賀坂祐平、星野由美
    • Organizer
      第54回日本卵子学会
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report
  • [Presentation] Key molecules active during oocyte maturation responsible for oocyte quality2010

    • Author(s)
      Yumi HOSHINO
    • Organizer
      FertiLink 2010 Key Issue Facing Fertility Specialists inAsia
    • Place of Presentation
      京都
    • Year and Date
      2010-10-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Key molecules active during oocyte maturation responsible for oocyte quality2010

    • Author(s)
      Yumi Hoshino
    • Organizer
      FertiLink 2010 Key Issue Facing Fertility Specialists in Asia
    • Place of Presentation
      京都(招待講演)
    • Year and Date
      2010-10-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] IVMの現状と将来:IVM卵子の成熟機構2010

    • Author(s)
      星野由美、佐藤優介、坂井知津香、佐藤英明
    • Organizer
      第28回日本受精着床学会総会・学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2010-07-29
    • Related Report
      2011 Final Research Report
  • [Presentation] IVMの現状と将来:IVM卵子の成熟機構2010

    • Author(s)
      星野由美、佐藤優介、坂井知津香、佐藤英明
    • Organizer
      第28回日本受精着床学会総会・学術集会
    • Place of Presentation
      横浜(招待講演)
    • Year and Date
      2010-07-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Inhibition of mTOR induces cumulus expansion and meiotic maturation in mouse oocytes without gonadotropin stimulation2010

    • Author(s)
      Yumi HOSHINO, Yusuke SATO, Chizuka SAKAI, Eimei SATO
    • Organizer
      The Society for Reproduction and Fertility Annual Conference 2010
    • Place of Presentation
      英国ノッティンガム
    • Year and Date
      2010-07-11
    • Related Report
      2011 Final Research Report
  • [Presentation] Inhibition of mTOR induces cumulus expansion and meiotic maturation in mouse oocytes without gonadotropin stimulation2010

    • Author(s)
      Yumi Hoshino, Yusuke Sato, Chizuka Sakai, Eimei Sato
    • Organizer
      The Society for Reproduction and Fertility Annual Conference 2010
    • Place of Presentation
      ノッティンガム、英国
    • Year and Date
      2010-07-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 卵成熟における紡錘体形成と染色体分配の分子メカニズム2010

    • Author(s)
      星野由美
    • Organizer
      日本臨床エンブリオロジスト学会
    • Place of Presentation
      東京
    • Year and Date
      2010-01-10
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] Functional role of Akt and mTOR in meiotic resumption and completion2009

    • Author(s)
      Yumi HOSHINO
    • Organizer
      International Ovarian Conference 2009
    • Place of Presentation
      東京
    • Year and Date
      2009-12-05
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Book] カラーアトラス不妊治療のための卵子学2010

    • Author(s)
      星野由美(鈴木秋悦編)
    • Publisher
      医歯薬学出版株式会社
    • Related Report
      2011 Final Research Report
  • [Book] カラーアトラス 不妊診療のための卵子学2010

    • Author(s)
      鈴木秋悦編
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://db.tohoku.ac.jp/whois/detail/48ad67dd1a6fbaa690afc373471de193.html

    • Related Report
      2011 Final Research Report
  • [Remarks] 東北大学大学院農学研究科動物生殖科学分野ホームページ

    • URL

      http://www.agri.tohoku.ac.jp/seisyoku/index-j.html

    • Related Report
      2011 Annual Research Report
  • [Remarks] 東北大学研究者紹介リンク

    • URL

      http://db.tohoku.ac.jp/whois/detail/48ad67dd1a6fbaa690afc373471de193.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.agri.tohoku.ac.jp/seisyoku/HoshinoPub.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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