Explolation of candidate genes associated with ooplsmic function

• Project/Area Number: 21780252
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied animal science
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: OBATA Yayoi Tokyo University of Agriculture, 応用生物科学部, 准教授 (70312907)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 発生工学 / 卵子 / 遺伝子発現 / 性分化 / 生殖細胞 / 細胞質成熟

Research Abstract

To explore candidate genes associated with ooplasmic function, we compared gene expression profile of GV oocytes derived from XX female with those derived from XY sex-reversed female. Transcripts of 92 genes showed>5-fold differences, including with BMP15, as known as essential gene for normal oocyte development.

Research Products

• [Journal Article] Epigenetically Immature Oocytes Lead to Loss of Imprinting During Embryogenesis. (2011)
  • Author(s): Obata Y, Hiura H, Fukuda A, Komiyama J, Hatada I, Kono T.
  • Journal Title: J Reprod Dev. 57(in press)
  • NAID: 10029050351
• [Journal Article] Study on the mechanism of maternal imprinting during oocyte growth. (2011)
  • Author(s): Obata Y.
  • Journal Title: J Reprod Dev. 57 Pages: 1-8
  • NAID: 10027900689
• [Journal Article] Epigenetically Immature Oocytes Lead to Loss of Imprinting During Embryogenesis. (2011)
  • Author(s): Obata Y, Hiura H, Fukuda A, Komiyama J, Hatada I, Kono T.
  • Journal Title: J Reprod Dev. Volume: 57(印刷中)
  • NAID: 10029050351
  • Peer Reviewed
• [Journal Article] Study on the mechanism of maternal imprinting during oocyte growth. (2011)
  • Author(s): Obata Y
  • Journal Title: J Reprod Dev. Volume: 57 Pages: 1-8
  • NAID: 10027900689
• [Journal Article] Deletion of Gtl2, imprinted non-coding RNA, with its differentially methylated region induces lethal parent-origin-dependent defects in mice. (2009)
  • Author(s): Takahashi N, Okamoto A, Kobayashi R, Shirai M., Obata Y, Ogawa H, Sotomaru Y, Kono T.
  • Journal Title: Hum Mol Genet. 8 Pages: 1879-1888
• [Journal Article] Deletion of Gtl2, imprinted non-coding RNA, with its differentially methylated region induces lethal parent-origin-dependent defects in mice. (2009)
  • Author(s): Takahashi N, Okamoto A, Kobayashi R, Shirai M, Obata Y, Ogawa H, Sotomaru Y, Kono T.
  • Journal Title: Hum Mol Genet. 8 Pages: 1879-1888
  • Peer Reviewed
• [Presentation] マウス非成長期卵母細胞におけるDNAメチル基転移酵素の発現誘導 (2010)
  • Author(s): 原聡史、高野喬、尾畑やよい、河野友宏
  • Organizer: 第103回日本繁殖生物学会
  • Place of Presentation: 北里大学(青森)
  • Year and Date: 2010-09-02
• [Presentation] XX型雌マウスとXY型性転換雌マウス由来卵母細胞における網羅的遺伝子発現解析 (2010)
  • Author(s): 尾畑やよい、曹峰、Teruko Taketo、Bao-Zeng Xu、河野友宏
  • Organizer: 第51回日本哺乳動物卵子学会
  • Place of Presentation: 朱鷺メッセ(新潟)
  • Year and Date: 2010-05-30
• [Presentation] マウス非成長期卵母細胞におけるDNAメチル基転移酵素の発現誘導 (2010)
  • Author(s): 原聡史、高野喬、尾畑やよい、河野友宏
  • Organizer: 日本繁殖生物学会
• [Presentation] 卵母細胞におけるゲノム刷込み機構に関する研究 (2010)
  • Author(s): 尾畑やよい
  • Organizer: 日本繁殖生物学会
• [Presentation] XX型雌マウスとXY型性転換雌マウス由来卵母細胞における網羅的遺伝子発現解析 (2010)
  • Author(s): 尾畑やよい、曹峰、Teruko Taketo、Bao-Zeng Xu、河野友宏
  • Organizer: 日本哺乳動物卵子学会
• [Presentation] 胚形成過程におけるゲノムインプリンティングの維持と消失 (2009)
  • Author(s): 尾畑やよい、河野友宏
  • Organizer: 日本繁殖生物学会
• [Presentation] 配偶子におけるepigenetics (2009)
  • Author(s): 尾畑やよい
  • Organizer: 日本哺乳動物卵子学会