Development of a new nuclear transfer technology using nuclear reprogramming inducing factors.

• Project/Area Number: 21780254
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied animal science
• Research Institution: Kinki University
• Principal Investigator: TANI Tetsuya Kinki University, 農学部, 講師 (70319763)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 核移植 / 核リプログラミング / 未受精卵子 / 体細胞核移植 / ブタ未受精卵子

Research Abstract

For enhancement of oocyte-base nuclear reprogramming potential of unfertilized oocytes, we injected nuclear reprogramming inducing factors (Oct3/4,sox2,klf4,c-Myc,Nanog,Gadd45a) for iPS cells establishment into pig unfertilized oocytes before nuclear transfer. However over-expressed oocyte of reprogramming inducing factors could not enhanced developmental potential of porcine nuclear transferred oocytes in vitro.

Research Products

• [Journal Article] Muscle master regulator MyoD, general activator VP16 and pioneer factor FoxA : why are they so powerful? (2011)
  • Author(s): Hirai H, Tani T, Kikyo N.
  • Journal Title: Int J Dev Biol Volume: (In press)
  • Peer Reviewed
• [Journal Article] Structure and functions of powerful transactivators : VP16, MyoD and FoxA. (2010)
  • Author(s): Hiroyuki H, Tani T, Kikyo N.
  • Journal Title: Int.J.Dev.Biol. 54巻 Pages: 1589-1596
• [Presentation] MECHANISM OF NUCLEAR REPROGRAMMING DURING IPS CELL GENERATION. (2010)
  • Author(s): Kellner Steven, Hirai Hiroyuki, Tani Tetsuya, Katoku-Kikyo Nobuko, Firpo Meri, Kikyo Nobuaki
  • Organizer: 8^<th> Internatinal Society for Stem Cell Reserch
  • Place of Presentation: サンフランシスコ米国
• [Presentation] メラトニンがブタ単為発生卵ならびに体細胞核移植卵の体外発生能に及ぼす影響 (2009)
  • Author(s): 中野真夕・谷哲弥・加藤容子・角田幸雄
  • Organizer: 第50回日本哺乳動物卵子学会
  • Place of Presentation: 東京
• [Presentation] ブタ体細胞核移植卵の体外発生能に及ぼすバブプロ酸の影響 (2009)
  • Author(s): 谷哲弥・加藤容子・角田幸雄
  • Organizer: 第110回日本畜産学会
  • Place of Presentation: 神奈川
• [Remarks] ホームページ等
  • URL: http://nara-kindai.unv.jp/02gakka/06bio/dobutsu/index.html