Development of a new nuclear transfer technology using nuclear reprogramming inducing factors.
| Project/Area Number |
21780254
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied animal science
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| Research Institution | Kinki University |
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Principal Investigator |
TANI Tetsuya Kinki University, 農学部, 講師 (70319763)
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| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 核移植 / 核リプログラミング / 未受精卵子 / 体細胞核移植 / ブタ未受精卵子 |
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| Research Abstract |
For enhancement of oocyte-base nuclear reprogramming potential of unfertilized oocytes, we injected nuclear reprogramming inducing factors (Oct3/4,sox2,klf4,c-Myc,Nanog,Gadd45a) for iPS cells establishment into pig unfertilized oocytes before nuclear transfer. However over-expressed oocyte of reprogramming inducing factors could not enhanced developmental potential of porcine nuclear transferred oocytes in vitro.
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Report
(3 results)
Research Products
(6 results)
