Characterization of abortion related factor of intracellular protozoan parasite Neospora caninum and its application for vaccine development

• Project/Area Number: 21780262
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: NISHIKAWA Yoshifumi Obihiro University of Agriculture and Veterinary Medicine, 原虫病研究センター, 准教授 (90431395)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 寄生虫 / 原虫 / ワクチン / 流産 / 垂直感染 / 家畜原虫病 / 家畜の流産 / ネオスポラ / 免疫

Research Abstract

The aim of this study is to characterize an abortion related factor of Neospora caninum, NcGRA7, for development of vaccine against neosporosis. This study showed that NcGRA7 could stimulate immune cells. Moreover, we developed vaccine based on NcGRA7 and could control the parasite infection in mouse model. The present study indicates the positive results for the vaccine development against neosporosis.

Research Products

• [Journal Article] Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice. (2010)
  • Author(s): Zhang G, Huang X, Boldbaatar D, Battur B, Battsetseg B, Zhang H, Yu L, Li Y, Luo Y, Cao S, Goo YK, Yamagishi J, Zhou J, Zhang S, Suzuki H, Igarashi I, Nishikawa Y, Xuan X.
  • Journal Title: Vaccine. 28(45) Pages: 7243-7247
  • Peer Reviewed
• [Journal Article] Roles of CD122^+ Cells in Resistance against Neospora caninum Infection in a Murine Model. (2010)
  • Author(s): Nishikawa Y, Zhang H, Ibrahim MH, Yamada K, Nagasawa H, Xuan X.
  • Journal Title: J Vet Med Sci. 72(10) Pages: 1275-1282
  • NAID: 130000264212
  • Peer Reviewed
• [Journal Article] Neospora caninum : Application of apical membrane antigen 1 encapsulated in the oligomannose-coated liposomes for reduction of offspring mortality from infection in BALB/c mice. (2010)
  • Author(s): Zhang H, Nishikawa Y, Yamagishi J, Zhou J, Ikehara Y, Kojima N, Yokoyama N, Xuan X.
  • Journal Title: Exp Parasitol. 125(2) Pages: 130-136
  • NAID: 120006390516
  • Peer Reviewed
• [Journal Article] Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice. (2010)
  • Author(s): Zhang G, et al.
  • Journal Title: Vaccine Volume: 28 Pages: 7243-7247
  • Peer Reviewed
• [Journal Article] Roles of CD122^+ Cells in Resistance against Neospora caninum Infection in a Murine Model. (2010)
  • Author(s): Nishikawa Y, et al.
  • Journal Title: J Vet Med Sci. Volume: 72 Pages: 1275-1282
  • NAID: 130000264212
  • Peer Reviewed
• [Journal Article] Application of apical membrane antigen 1 encapsulated in the oligomannose-coated liposomes for reduction of offspring mortality from infection in BALB/c mice. (2010)
  • Author(s): Zhang H, et al.
  • Journal Title: Exp Parasitol. Volume: 125 Pages: 130-136
  • Peer Reviewed
• [Journal Article] 総説「ネオスポラ症に対する防御方法の開発に向けて」獣医畜産新報 (2009)
  • Author(s): 西川義文
  • Journal Title: Journal of Veterinary Medicine 62(5) Pages: 383-388
• [Journal Article] Immunization of oligomannose-coated liposome-entrapped NcGRA7 protects dams and offspring from Neospora caninum infection in mice. (2009)
  • Author(s): Nishikawa Y, Zhang H, Ikehara Y, Kojima N, Xuan X, Yokoyama N.
  • Journal Title: Clin Vaccine Immunol. 16(6) Pages: 792-797
  • Peer Reviewed
• [Journal Article] Effects of a transferring antibody against Neospora caninum infection in a murine model. (2009)
  • Author(s): Nishikawa Y, Zhang H, Huang P, Zhang G, Xuan X.
  • Journal Title: Vet Parasitol. 160(1-2) Pages: 60-65
  • Peer Reviewed
• [Journal Article] 総説「ネオスポラ症に対する防御方法の開発に向けて」 (2009)
  • Author(s): 西川義文
  • Journal Title: 獣医畜産新報 Volume: 27 Pages: 383-388
• [Journal Article] Immunization of oligomannose-coated liposome-entrapped NcGRA7 protects dams and offspring from Neospora caninum infection in mice. (2009)
  • Author(s): Nishikawa Y, et al.
  • Journal Title: Clin Vaccine Immunol. Volume: 16 Pages: 792-797
  • Peer Reviewed
• [Journal Article] Effects of a transferring antibody against Neospora caninum infection in a murine model. (2009)
  • Author(s): Nishikawa Y, et al.
  • Journal Title: Vet Parasitol. Volume: 160 Pages: 60-65
  • Peer Reviewed
• [Presentation] ネオスポラ感染動態における原虫由来分子サイクロフィリンの役割 (2010)
  • Author(s): 西川義文, ら
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2010-09-18
• [Presentation] ウシにおけるネオスポラ感染モデルの作出 (2010)
  • Author(s): 小原潤子, ら
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2010-09-18
• [Presentation] ネオスポラ特異的移行抗体は原虫感染の防御に関与しない (2010)
  • Author(s): 西川義文, ら
  • Organizer: 第149回日本獣医学会学術集会
  • Place of Presentation: 日本獣医生命科学大学
  • Year and Date: 2010-03-28
• [Presentation] ネオスポラApical Membrane Antigen-1(NcAMA1)によるネオスポラ垂直感染に対する防御効果 (2009)
  • Author(s): 西川義文, ら
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: とぎりん文化会館
  • Year and Date: 2009-09-26
• [Presentation] オリゴ糖リポソームを用いたネオスポラ垂直感染に対するワクチン開発 (2009)
  • Author(s): 西川義文, ら
  • Organizer: 第147回日本獣医学会学術集会
  • Place of Presentation: 栃木県総合文化センター
  • Year and Date: 2009-04-03
• [Remarks] 原虫病研究センターHP
  • URL: http://www.obihiro.ac.jp/~protozoa/index.html
• [Remarks] 研究室HP
  • URL: http://www.obihiro.ac.jp/~geneticbiochem/index.html
• [Remarks] 原虫病研究センターHP
  • URL: http://www.obihiro.ac.jp/~protozoa/index.html
• [Remarks] 研究室HP
  • URL: http://www.obihiro.ac.jp/~geneticbiochem/index.html
• [Remarks] 原虫病研究センターHP
  • URL: http://www.obihiro.ac.jp/~protozoa/index.html
• [Remarks] 研究室HP
  • URL: http://www.obihiro.ac.jp/~geneticbiochem/index.html
• [Patent(Industrial Property Rights)] ネオスポラ原虫感染症に対するワクチン製剤 (2009)
  • Inventor(s): 西川義文、横山直明、小島直也
  • Industrial Property Rights Holder: 帯広畜産大学、東海大学
  • Filing Date: 2009-09-11
  • Overseas
• [Patent(Industrial Property Rights)] ネオスポラ原虫感染症に対するワクチン製剤 (2009)
  • Inventor(s): 西川義文、横山直明、小島直也
  • Industrial Property Rights Holder: 帯広畜産大学、東海大学
  • Filing Date: 2009-09-11
  • Overseas