| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
In order to develop the novel technique to prevent the bacterial infection, I tried to synthesize the recombinant protein of receptor for quorum sensing-signaling molecule (AHL), LasR. The analysis of amino acids of cloned lasR gene indicated that LasR consists of AHL-binding domain and DNA-binding domain. The recombinant LasR was successfully synthesized by in vitro system using insect cell extraction, while LasR was rapidly digested when the protein was synthesized in E. coli competent cells or cultured cells. In addition, the effect of AHL to promote the wound healing was discovered during the process of this research.
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