| Project/Area Number |
21780265
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Tottori University |
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Principal Investigator |
HOSAKA Yoshinao Tottori University, 農学部, 准教授 (00337023)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 間葉系幹細胞 / 骨分化 / コンドロイチン硫酸 / 骨の分化と再生 / 細胞外マトリクス / CS-E / 高硫酸化多糖 / BMP-4 / スキャフォールド / ヘパリン / 細胞マトリクス / インタラクション / BMP / FGF |
|---|
| Research Abstract |
In this study, we identified CS/DS sugar chain, which closely related to bone differentiation, and then analyzed its functions. Experiments using cultured cells, five types CS/DS (CS-A, -B, -C, -D and -E) chain were compared the ALP activity and calcium deposition and resulted CS-E had the strongest ability to induce bone differentiation. The CS-E-containing sponges (2ug/defect) were implanted in the site of bone defects created in Wistar rat skull, the bone regeneration was confirmed. The rate of bone regeneration in a 15-week experimental period was approximately 30% of defects.
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