Structural bioinformatics analysis of antigenic changes in influenza virus hemagglutinin

• Project/Area Number: 21780272
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied veterinary science
• Research Institution: Hokkaido University
• Principal Investigator: IGARASHI Manabu Hokkaido University, 人獣共通感染症サーチセンター, 特任助教 (10374240)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 人畜共通感染症 / インフルエンザ / ウイルス / 感染症 / 生体生命情報学 / 計算科学 / 分子シミュレーション / 糖鎖 / 蛋白質

Research Abstract

The hemagglutinin (HA) of influenza A viruses undergoes antigenic changes to escape from antibody-mediated immune pressure. In order to control for influenza, it is important to understand the patterns of amino acid substitutions in the past and then to predict possible antigenic changes of the HA molecules in future. In this study, we analyzed the change of HA antigenic structures in the evolutionary process of influenza viruses in the human population, by using structural bioinformatics approach. Based on the results, we predicted possible amino acid substitutions that might be associated with future antigenic change of 2009 H1N1.

Research Products

• [Journal Article] Characterization of influenza A viruses isolated from wild waterfowls in Zambia (2011)
  • Author(s): Simulundu E, Ishii A, Igarashi M, Mweene A, Suzuki Y, Hang'ombe B, Namangala B, Moonga L, Manzoor R, Ito K, Nakamura I, Sawa H, Sugimoto C, Kida H, Simukonda C, Chansa W, Chulu J, Takada A
  • Journal Title: J Gen Virol. in press
  • Peer Reviewed
• [Journal Article] Involvement of viral envelope GP2 in Ebola virus entry into cells expressing the macrophage galactose-type C-type lectin. (2011)
  • Author(s): Usami K, Matsuno K, Igarashi M, Denda-Nagai K, Takada A, Irimura T
  • Journal Title: Biochem Biophys Res Commun 407 Pages: 74-78
  • Peer Reviewed
• [Journal Article] Prediction of the antigenic changes of the pandemic (H1N1) 2009 influenza virus hemagglutinin. (2011)
  • Author(s): Igarashi M, Ito K, Yoshida R, Tomabechi D, Kida H, Takada A
  • Journal Title: Influenza and other respiratory viruses 5(supple.1) Pages: 402-404
  • Peer Reviewed
• [Journal Article] Genetic characterization and susceptibility on poultry and mammal of H7N6 subtype avian influenza virus isolated in Japan in 2009. (2011)
  • Author(s): Uchida Y, Kanehira K, Mase M, Takemae N, Watanabe C, Ito T, Igarashi M, Ito K, Takada A, Sakoda Y, Okamatsu M, Yamamoto Y, Nakamura K, Kida H, Hiromoto Y, Tsuda T, Saito T
  • Journal Title: Vet Microbiol. 147(1-2) Pages: 1-10
  • Peer Reviewed
• [Journal Article] Genetic characterization and susceptibility on poultry and mammal of H7N6 subtype avian influenza virus isolated in Japan in 2009 (2011)
  • Author(s): Uchida Y, Kanehira K, Mass M, Takemae N, Watanabe C, Ito T, Igarashi M, Ito K, Takada A, Sakoda Y, Okamatsu M, Yamamoto Y, Nakamura K, Kida H, Hiromoto Y, Tsuda T, Saito T
  • Journal Title: Vet Microbiol. Volume: 147(1-2) Pages: 1-10
  • Peer Reviewed
• [Journal Article] Involvement of viral envelope GP2 in Ebola virus entry into cells expressing the macrophage galactose-type C-type lectin (2011)
  • Author(s): Usami K, Matsuno K, Igarashi M, Denda-Nagai K, Takada A, Irimura T
  • Journal Title: Biochem Biophys Res Commun Volume: (in press)
  • Peer Reviewed
• [Journal Article] Characterization of influenza A viruses isolated from wild waterfowls in Zambia (2011)
  • Author(s): Simulundu E, Ishii A, Igarashi M, Mweene A, Suzuki Y, Hang'ombe B, Namangala B, Moonga L, Manzoor R, Ito K, Nakamura I, Sawa H, Sugimoto C, Kida H, Simukonda C, Chansa W, Chulu J, Takada A
  • Journal Title: J Gen Virol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Construction of an infectious cDNA clone for Omsk Hemorrhagic fever virus, and characterization of mutations in NS2A and NS5. (2010)
  • Author(s): Yoshii K, Igarashi M, Ito K, Kariwa H, Holbrook MR, Takashima I
  • Journal Title: Virus Research 155(1) Pages: 61-68
  • NAID: 120002834769
  • Peer Reviewed
• [Journal Article] Enzyme-linked immunosorbent assay for the detection of filovirus species-specific antibodies. (2010)
  • Author(s): Nakayama E, Yokoyama A, Miyamoto H, Igarashi M, Kishida N, Matsuno K, Marzi A, Feldmann H, Ito K, Saijo M, Takada A
  • Journal Title: Clinical and Vaccine Immunology 17(11) Pages: 1723-1728
  • NAID: 120002973852
  • Peer Reviewed
• [Journal Article] Transcellular transport of West Nile virus-like particles across human endothelial cells depends on residues 156 and 159 of envelope protein. (2010)
  • Author(s): Hasebe R, Suzuki T, Makino Y, Igarashi M, Yamanouchi S, Maeda A, Horiuchi M, Sawa H, Kimura T
  • Journal Title: BMC Microbiol. 10(1) Pages: 165-165
  • NAID: 120002261736
  • Peer Reviewed
• [Journal Article] J.Virol. (2010)
  • Author(s): Matsuno K, Kishida N, Usami K, Igarashi M, Yoshida R, Nakayama E, Shimojima M, Feldmann H, Irimura T, Kawaoka Y, Takada A
  • Journal Title: Different potential of C-type lectin-mediated entry between Marburg virus strains. 84(10) Pages: 5140-5147
  • Peer Reviewed
• [Journal Article] A novel copper(II) coordination at His186 in full-length murine prion protein. (2010)
  • Author(s): Watanabe Y, Hiraoka W, Igarashi M, Ito K, Shimoyama Y, Horiutchi M, Yamamori T, Yasui H, Kuwabara M, Inagaki F, Inanam O
  • Journal Title: Biochem Biophys Res Commun 394(3) Pages: 522-528
  • NAID: 120002148672
  • Peer Reviewed
• [Journal Article] Predicting the Antigenic Structure of the Pandemic (H1N1) 2009 Influenza Virus Hemagglutinin. (2010)
  • Author(s): Igarashi M, Ito K, Yoshida R, Tomabechi D, Kida H, Takada A
  • Journal Title: PLoS One 5(1)
  • NAID: 120001804925
  • Peer Reviewed
• [Journal Article] Transcellular transport of West Nile virus-like particles across human endothelial cells depends on residues 156 and 159 of envelope protein (2010)
  • Author(s): Hasebe R, Suzuki T, Makino Y, Igarashi M, Yamanouchi S, Maeda A, Horiuchi M, Sawa H, Kimura T
  • Journal Title: BMC Microbiol Volume: 10(1) Pages: 165-165
  • NAID: 120002261736
  • Peer Reviewed
• [Journal Article] Enzyme-linked immunosorbent assay for the detection of filovirus species-specific antibodies (2010)
  • Author(s): Nakayama E, Yokoyama A, Miyamoto H, Igarashi M, Kishida N, Matsuno K, Marzi A, Feldmann H, Ito K, Saijo M, Takada A
  • Journal Title: Clinical and Vaccine Immunology Volume: 17(11) Pages: 1723-1728
  • NAID: 120002973852
  • Peer Reviewed
• [Journal Article] Construction of an infectious cDNA clone for Omsk Hemorrhagic fever virus, and characterization of mutations in NS2A and NS5 (2010)
  • Author(s): Yoshii K, Igarashi M, Ito K, Kariwa H, Holbrook MR, Takashima I
  • Journal Title: Virus Research Volume: 155(1) Pages: 61-68
  • NAID: 120002834769
  • Peer Reviewed
• [Journal Article] Predicting the Antigenic Structure of the Pandemic(H1N1)2009 Influenza Virus Hemagglutinin (2010)
  • Author(s): Manabu Igarashi, Kimihito Ito, Reiko Yoshida, Daisuke Tomabechi, Hiroshi Kida, Ayato Takada
  • Journal Title: PLoS One 5(1)
  • NAID: 120001804925
  • Peer Reviewed
• [Journal Article] A novel copper(II)coordination at His186 in full-length murine prion protein (2010)
  • Author(s): Yasuko Watanabe, Wakako Hiraoka, Manabu Igarashi, Kimihito Ito, Yuhei Shimoyama, Motohiro Horiutchi, Tohru Yamamori, Hironobu Yasui, Mikinori Kuwabara, Fuyuhiko Inagaki, Osamu Inanam
  • Journal Title: Biochem Biophys Res Commun (in press)
  • NAID: 120002148672
  • Peer Reviewed
• [Journal Article] Different potential of C-type lectin-mediated entry between Marburg virus strains (2010)
  • Author(s): Keita Matsuno, Noriko Kishida, Katsuaki Usami, Manabu Igarashi, Reiko Yoshida, Eri Nakayama, Masayuki Shimojima, Heinz Feldmann, Tatsuro Irimura, Yoshihiro Kawaoka, Ayato Takada
  • Journal Title: J.Virol (in press)
  • Peer Reviewed
• [Presentation] パンデミックインフルエンザAウイルス(H1N1)ヘマグルチニンに対するモノクローナル抗体の性状解析 (2010)
  • Author(s): 吉田玲子, 他
  • Organizer: 第58回日本ウイルス学術集会
  • Place of Presentation: 徳島(徳島県郷土文化会館)
  • Year and Date: 2010-10-08
• [Presentation] フィロウイルス種特異抗体検出ELISA法の開発 (2010)
  • Author(s): 中山絵里, 他
  • Organizer: 第58回日本ウイルス学術集会
  • Place of Presentation: 徳島(徳島県郷土文化会館)
  • Year and Date: 2010-10-08
• [Presentation] H3N2インフルエンザウイルスHAに付加された糖鎖は周辺エピトープを覆い隠していたか?～糖鎖付加部位周辺アミノ酸残基の多様性変化の解析～ (2010)
  • Author(s): 五十嵐学, 他
  • Organizer: 第58回日本ウイルス学術集会
  • Place of Presentation: 徳島(徳島県郷土文化会館)
  • Year and Date: 2010-10-08
• [Presentation] パンデミックインフルエンザA ウイルス(H1N1)ヘマグルチニンに対するモノクローナル抗体の性状解析 (2010)
  • Author(s): 吉田玲子、苫米地大輔、五十嵐学、宮本洋子、加瀬哲男、喜田宏、高田礼人
  • Organizer: 第58回日本ウイルス学術集会
  • Place of Presentation: 徳島(徳島県郷土文化会館)
  • Year and Date: 2010-10-08
• [Presentation] Prediction of the Antigenic Change of the Pandemic (H1N1) 2009 Influenza Virus Hemagglutinin (2010)
  • Author(s): Igarashi M, 他
  • Organizer: Options for the Control of Influenza VII
  • Place of Presentation: China (Hong Kong SAR)
  • Year and Date: 2010-09-06
• [Presentation] Prediction of Antigenic Structure of Hemagglutinin of Pandemic (H1N1) 2009 Influenza Virus (2010)
  • Author(s): Ito K, 他
  • Organizer: 14th International Negative Strand Virus Meeting
  • Place of Presentation: Belgium (Bruges)
  • Year and Date: 2010-06-23
• [Presentation] インフルエンザウイルスの抗原変異予測と亜型間交差感染防御 (2010)
  • Author(s): 高田礼人, 他
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山(就実大学)
  • Year and Date: 2010-03-28
• [Presentation] ホモロジーモデリング法による新型H1N1インフルエンザウイルスのヘマグルチニンの抗原構造の解析 (2009)
  • Author(s): 五十嵐学, 他
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京(都市センターホテル)
  • Year and Date: 2009-10-26
• [Presentation] インフルエンザウイルスのヘマグルチニンのMDS解析と変異予測への応用 (2009)
  • Author(s): 伊藤公人, 他
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京(都市センターホテル)
  • Year and Date: 2009-10-26
• [Presentation] 愛知県でウズラから分離されたH7N6亜型鳥インフルエンザウイルスの遺伝子解析 (2009)
  • Author(s): 内田裕子, 他
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京(都市センターホテル)
  • Year and Date: 2009-10-26
• [Presentation] MGL/CD301を介するエボラウイルス感染増強に関わる表層糖蛋白質の構造的特徴 (2009)
  • Author(s): 宇佐美克明, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸(ポートアイランド)
  • Year and Date: 2009-10-24
• [Presentation] マウスプリオンタンパク質ヒスチジン186周辺の銅イオン結合部位に関する構造解析 (2009)
  • Author(s): 稲波修, 他
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取(とりぎん文化会館)
  • Year and Date: 2009-09-25
• [Presentation] インフルエンザウイルスのヘマグルチニンの進化解析と変異予測への応用 (2009)
  • Author(s): 伊藤公人, 他
  • Organizer: 第11回日本進化学会大会
  • Place of Presentation: 札幌(北海道大学)
  • Year and Date: 2009-09-03
• [Presentation] インフルエンザウイルスヘマグルチニンの糖鎖付加ポテンシャルの予測 (2009)
  • Author(s): 五十嵐学, 他
  • Organizer: 第23回インフルエンザ研究者交流の会シンポジウム
  • Place of Presentation: 東京(東京大学医科学研究所)
  • Year and Date: 2009-07-03
• [Presentation] インフルエンザウイルスHA亜型間交差中和活性を有するモノクローナル抗体 (2009)
  • Author(s): 吉田玲子, 他
  • Organizer: 第23回インフルエンザ研究者交流の会シンポジウム
  • Place of Presentation: 東京(東京大学医科学研究所)
  • Year and Date: 2009-07-03