Expression analysis of endogenous retroviral elements in canine malignant tumors.

• Project/Area Number: 21780288
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Clinical veterinary science
• Research Institution: Yamaguchi University
• Principal Investigator: BABA Kenji Yamaguchi University, 農学部, 助教 (90452367)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 内在性レトロウイルス / 悪性腫瘍 / 犬 / イヌ / 悪性黒色腫 / 腫瘍

Research Abstract

An endogenous retroviral envelope gene was newly identified in canine genome. The predicted amino acid sequence indicated that the retroviral element belonged to the genus Betaretrovirus and encoded a functional envelope protein. The expression of the envelope mRNA was shown in canine malignant melanoma cell lines. This study indicated that the newly identified retroviral envelope may be a tumor antigen in canine malignant melanoma.

Research Products

• [Journal Article] Identification of novel endogenous betaretroviruses which are transcribed in the bovine placenta. (2011)
  • Author(s): Baba K, Nakaya Y, Shojima T, Muroi Y, Kizaki Y, Hashizume K, Imakawa K, Miyazawa T.
  • Journal Title: Journal of Virology 85(3) Pages: 1237-1245
  • Peer Reviewed
• [Journal Article] Adaptation of feline immunodeficiency virus subtype B strain TM2 to a feline astrocyte cell line (G355-5 cells). (2011)
  • Author(s): Ishikawa M
  • Journal Title: Veterinary Microbiology Volume: 149(3-4) Pages: 307-315
  • Peer Reviewed
• [Journal Article] Identification of novel endogenous betaretroviruses which are transcribed in the bovine placenta. (2011)
  • Author(s): Baba K
  • Journal Title: Journal of Virology Volume: 85(3) Pages: 1237-1245
  • Peer Reviewed
• [Journal Article] Susceptibility and production of a feline endogenous retrovirus (RD-114 virus) in various feline cell lines. (2011)
  • Author(s): Okada M
  • Journal Title: Virus Research Volume: 155(1) Pages: 268-273
  • Peer Reviewed
• [Journal Article] Differential Diagnosis of Feline Leukemia Virus Subgroups Using Pseudotype Viruses Expressing Green Fluorescent Protein. (2010)
  • Author(s): Nakamura M
  • Journal Title: The Journal of Veterinary Medical Science (In press)
  • Peer Reviewed
• [Journal Article] Antiviral activity of membrane fusion inhibitors that target gp40 of the feline immunodeficiency virus envelope protein. (2009)
  • Author(s): Mizukoshi F
  • Journal Title: Veterinary Microbiology 136(1-2) Pages: 155-159
  • Peer Reviewed
• [Journal Article] Prevalence of hematological abnormalities and detection of infected bone marrow cells in asymptomatic cats with feline immunodeficiency virus infection. (2009)
  • Author(s): Fujino Y
  • Journal Title: Veterinary Microbiology 36(3-4) Pages: 217-225
  • Peer Reviewed
• [Journal Article] Characterization of monocyte-derived dendritic cells from cats infected with feline immunodeficiency virus. (2009)
  • Author(s): Mizukoshi F
  • Journal Title: The Journal of Veterinary Medical Science 71(7) Pages: 865-871
  • Peer Reviewed
• [Presentation] 見落としがちな感染症～診断のポイント～ (2010)
  • Author(s): 馬場健司
  • Organizer: 日本獣医内科学アカデミー/日本獣医臨床病理学会 2010年大会
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 2010-02-12
• [Presentation] Identification of Novel Bovine Endogenous Retrovirus Envelopes and Putative Accessory Proteins. (2009)
  • Author(s): Baba K, Shojima T, Miyazawa T.
  • Organizer: 21st International Workshop on Retroviral Pathogenesis
  • Place of Presentation: Italy Ciocco Hotel and Rerort Lucca.
  • Year and Date: 2009-09-14
• [Presentation] Identification of Novel Bovine Endogenous Retrovirus Envelopes and Putative Accessory Proteins. (2009)
  • Author(s): Baba K
  • Organizer: 21st International Workshop on Retroviral Pathogenesis
  • Place of Presentation: Il Ciocco Hotel and Resort, Lucca, Italy
  • Year and Date: 2009-09-14
• [Remarks] ホームページ等