Establishment of feline embryonic stem cell derived from developmental engineering

• Project/Area Number: 21780289
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Clinical veterinary science
• Research Institution: Osaka Prefecture University
• Principal Investigator: HATOYA Shingo Osaka Prefecture University, 生命環境科学研究科, 助教 (40453138)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 再生医療 / ネコ / ES細胞 / 体外受精 / 卵子 / 体外培養 / LIF / 精子 / BCB / パーコール

Research Abstract

The objective of this study was to establish ES cell lines from the cat for regenerative veterinary medicine. I examined feline IVM/IVF/IVC and establishment of feline embryonic stem cells, and the following results were obtained.
(1)The BCB(+) oocytes reached more MII and morula/blastocysts than BCB (-) oocytes.
(2)The isolation of feline sperm by percoll density gradient is effective in selecting the useful sperm.
(3)Sequence alignment and comparison of feline LIF cDNA with human and mouse LIF cDNA revealed a high degree of nucleotide similarity.
(4)The IVC of Only-one medium+FBS developed the feline embryo up to blastocyst stage.
(5)Primary feline ES-like cell colonies were formed from IVF derived blastocysts.

Research Products

• [Journal Article] Successful Management with CHOP for Pulmonary Lymphomatoid Granulomatosis in a Dog. (2011)
  • Author(s): Hatoya S, Kumagai D, Takeda S, Yamamoto E, Nakanishi M, Kuwamura M, Sugiura K, Sasai H, Yamate J, Inaba T.
  • Journal Title: J Vet Med Sci. 73(4) Pages: 527-30
  • NAID: 130000444583
  • Peer Reviewed
• [Journal Article] Comparison of testosterone and insulin-like peptide 3 secretions in response to human chorionic gonadotropin in cultured interstitial cells from scrotal and retained testes in dogs. (2011)
  • Author(s): Pathirana IN, Ashida Y, Kawate N, Tanaka K, Tsuji M, Takahashi M, Hatoya S, Inaba T, Tamada H.
  • Journal Title: Anim Reprod Sci. 124(1-2) Pages: 138-144
  • Peer Reviewed
• [Journal Article] Comparison of testosterone and insulin-like peptide 3 secretions in response to human chorionic gonadotropin in cultured interstitial cells from scrotal and retained testes in dogs. (2011)
  • Author(s): Pathirana IN, Ashida Y, Kawate N, Tanaka K, Tsuji M, Takahashi M, Hatoya S, Inaba T, Tamada H.
  • Journal Title: Anim Reprod Sci. Volume: 124(1-2) Pages: 138-144
  • Peer Reviewed
• [Journal Article] Successful Management with CHOP for Pulmonary Lymphomatoid Granulomatosis in a Dog. (2011)
  • Author(s): Hatoya S, Kumagai D, Takeda S, Yamamoto E, Nakanishi M, Kuwamura M, Sugiura K, Sasai H, Yamate J, Inaba T.
  • Journal Title: J Vet Med Sci. Volume: 73(4) Pages: 527-530
  • NAID: 130000444583
  • Peer Reviewed
• [Journal Article] IFN-gamma markedly cooperates with intratumoral dendritic cell vaccine in dog tumor models. (2010)
  • Author(s): Mito K, Sugiura K, Ueda K, Hori T, Akazawa T, Yamate J, Nakagawa H, Hatoya S, Inaba M, Inoue N, Ikehara S, Inaba T.
  • Journal Title: Cancer Res. 70(18) Pages: 7093-7101
  • Peer Reviewed
• [Journal Article] Effect of IL-12 on the canine dendritic cell maturation following differentiation induced by granulocyte-macrophage CSF and IL-4. (2010)
  • Author(s): Sugiura K., Wijewardana V., Fujimoto M., Akazawa T., Yahata M., Mito K., Hatoya S., Inoue N., Inaba T.
  • Journal Title: Vet.Immunol.Immunopathol. 137(3-4) Pages: 322-326
  • Peer Reviewed
• [Journal Article] Detection of relaxin mRNA in the corpus luteum, uterus, and uterine cervix in the bitch. (2010)
  • Author(s): Tamada H, Kawate N, Kawata N, Inaba T, Kida K, Hatoya S, Akune A, Nakama K, Kohsaka T, Sawada T.
  • Journal Title: J Vet Med Sci. 72(10) Pages: 1383-1386
  • NAID: 130000264202
  • Peer Reviewed
• [Journal Article] Analysis of single nucleotide polymorphisms in the 3' region of the estrogen receptor 1 gene in normal and cryptorchid Miniature Dachshunds and Chihuahuas. (2010)
  • Author(s): Pathirana IN, Tanaka K, Kawate N, Tsuji M, Kida K, Hatoya S, Inaba T, Tamada H.
  • Journal Title: J Reprod Dev. 56(4) Pages: 405-410
  • NAID: 10026621610
  • Peer Reviewed
• [Journal Article] Epidermal growth factor, transforming growth factor-alpha, and epidermal growth factor receptor expression and localization in the canine endometrium during the estrous cycle and in bitches with pyometra. (2010)
  • Author(s): Kida K, Maezono Y, Kawate N, Inaba T, Hatoya S, Tamada H.
  • Journal Title: Theriogenology. 73(1) Pages: 36-47
  • Peer Reviewed
• [Journal Article] IFN{gamma} markedly cooperates with intratumoral dendritic cell vaccine in dog tumor models. (2010)
  • Author(s): Mito K, Sugiura K, Ueda K, Hori T, Akazawa T, Yamate J, Nakagawa H, Hatoya S, Inaba M, Inoue N, Ikehara S, Inaba T.
  • Journal Title: Cancer Res. Volume: 70(18) Pages: 7093-7101
  • Peer Reviewed
• [Journal Article] Effect of IL-12 on canine dendritic cell maturation following differentiation induced by granulocyte-macrophage CSF and IL-4. (2010)
  • Author(s): Sugiura K, Wijewardana V, Fujimoto M, Akazawa T, Yahata M, Mito K, Hatoya S, Inoue N, Inaba T.
  • Journal Title: Vet Immunol Immunopathol. Volume: 137(3-4) Pages: 322-326
  • Peer Reviewed
• [Journal Article] Detection of relaxin mRNA in the corpus luteum, uterus, and uterine cervix in the bitch. (2010)
  • Author(s): Tamada H, Kawate N, Kawata N, Inaba T, Kida K, Hatoya S, Akune A, Nakama K, Kohsaka T, Sawada T.
  • Journal Title: J Vet Med Sci. Volume: 72(10) Pages: 1383-1386
  • NAID: 130000264202
  • Peer Reviewed
• [Journal Article] Analysis of single nucleotide polymorphisms in the 3' region of the estrogen receptor 1 gene in normal and cryptorchid Miniature Dachshunds and Chihuahuas. (2010)
  • Author(s): Pathirana IN, Tanaka K, Kawate N, Tsuji M, Kida K, Hatoya S, Inaba T, Tamada H.
  • Journal Title: J Reprod Dev. Volume: 56(4) Pages: 405-410
  • NAID: 10026621610
  • Peer Reviewed
• [Journal Article] Epidermal gmwth factor, transfornming growth factor-alpha, and epidermal growth factor receptor expression and localization in the canine endometrium during the eatroua cycle and in bitches with pyometra. (2010)
  • Author(s): Kida K, Maezono Y, Kawate N, Inaba T, Hato Tamada H.
  • Journal Title: Theriogenology 73(1) Pages: 36-47
  • Peer Reviewed
• [Journal Article] Canine oocyte maturation in culture : Significance of estrogen and EGF receptor gene expression in cumulus cells. (2009)
  • Author(s): Hatoya S., Sugiyama Y., Nishida H., Okuno T., Torii R., Sugiura K., Kida K., Kawate N., Tamada H., Inaba T.
  • Journal Title: Theriogenology 71(4) Pages: 560-567
  • Peer Reviewed
• [Journal Article] Locomotor improvement after autologous bone marrow stromal cell intrathecal delivery in dogs with chronic spinal cord injury.
  • Author(s): Nishida H, Nakayama M, Tanaka H, Kitamura M, Hatoya S, Sugiura K, Suzuki Y, Ide C, Inaba T.
  • Journal Title: Am J Vet Res in press
  • Peer Reviewed
• [Journal Article] Induction of fertile oestrus in dioestrous bitches by prostaglandin F2 α and a GnRH-agonist.
  • Author(s): Ohmura M, Torii R, Hatoya S, Sugiura K, Tamada H, Kawate N, Takahashi M, Sawada T, Inaba T.
  • Journal Title: Vet Rec in press
  • Peer Reviewed
• [Journal Article] Locomotor improvement after autologous bone marrow stromal cell intrathecal delivery in dogs with chronic spinal cord injury.
  • Author(s): Nishida H, Nakayama M, Tanaka H, Kitamura M, Hatoya S, Sugiura K, Suzuki Y, Ide C, Inaba T.
  • Journal Title: Am J Vet Res Volume: (accepted)
  • Peer Reviewed
• [Presentation] サルES細胞からのオリゴデンドロサイトへの簡便な分化誘導法 (2010)
  • Author(s): 瀧野麻衣亜、奥野剛、鳩谷晋吾、大村雅、鳥居隆三、杉浦喜久弥、玉田尋通、川手憲俊、高橋正弘、稲葉俊夫
  • Organizer: 日本獣医学会第150回大会
  • Place of Presentation: 帯広
  • Year and Date: 2010-09-17
• [Presentation] FSHおよびLHによるイヌ卵子の体外成熟率の改善 (2010)
  • Author(s): 永見俊典、鳩谷晋吾、鳥居隆三、杉浦喜久弥、大村雅、川手憲俊、玉田尋通、高橋正弘、稲葉俊夫
  • Organizer: 日本獣医学会第150回大会
  • Place of Presentation: 帯広
  • Year and Date: 2010-09-16
• [Presentation] 再生医療とは?基礎から動物のES・iPS細胞研究まで (2010)
  • Author(s): 鳩谷晋吾
  • Organizer: 日本小動物獣医師会2010年次学会
  • Place of Presentation: 東京
  • Year and Date: 2010-08-28
• [Presentation] 再生医療の最前線～獣医臨床応用に向けた課題～ (2010)
  • Author(s): 鳩谷晋吾
  • Organizer: 「幹細胞:犬ES細胞(胚性幹細胞)の開発」第149回日本獣医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-27
• [Presentation] 再生医療の最前線～獣医臨床応用に向けた課題～「幹細胞:犬ES細胞(胚性幹細胞)の開発」 (2010)
  • Author(s): 鳩谷晋吾
  • Organizer: 第149回 日本獣医学会学術集会
  • Place of Presentation: 日本獣医生命科学大学(東京)
  • Year and Date: 2010-03-27