Studies on degradation of endocrine disruptors by white-rot fungi and molecular breeding of hyper endocrine disruptors-degrading fungi in contaminated soils

• Project/Area Number: 21780296
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Boundary agriculture
• Research Institution: Shizuoka University
• Principal Investigator: HIRAI Hirofumi 静岡大学, 農学部, 准教授 (70322138)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 環境浄化 / 白色腐朽菌 / 環境ホルモン / 分解機構 / 分子育種 / プロテオーム解析

Research Abstract

The degradation of bisphenol A(BPA) was demonstrated with hyper lignin-degrading white-rot fungus Phanerochaete sordida YK-624. BPA was oxidatively polymerized under ligninolytic condition. On the other hand, BPA was hydroxylated under non-ligninolytic condition. It is suggested that cytochrome P450 is involved in this hydroxylation. High manganese peroxidase-producing transformant was able to eliminate BPA-contaminated soil by approximately 90% for 5 day-incubation.

Research Products

• [Journal Article] Effective Removal of Endocrine-Disrupting Compounds by Lignin Peroxidase from the White-Rot Fungus Phanerochaete sordidaYK-624 (2012)
  • Author(s): Jianqiao Wang, Nayumi Majima, Hirofumi Hirai, Hirokazu Kawagishi
  • Journal Title: Current Microbiology Volume: 64 Pages: 300-303
• [Journal Article] Effective removal of endocrine disrupting-compounds by lignin peroxidase from the white-rot fungus Phanerochaete sordida YK-624 (2012)
  • Author(s): J.Wang, N.Majima, H.Hirai, H.Kawagishi
  • Journal Title: Current Microbiology Volume: 64 Issue: 3 Pages: 300-303
  • DOI: 10.1007/s00284-011-0067-2
  • Peer Reviewed
• [Presentation] 高活性リグニン分解菌Phanerochaete sordida YK-624株によるビスフェノールAの分解 (2012)
  • Author(s): 王剣橋、河岸洋和、山本陽太郎、山本涼子、平井浩文
  • Organizer: 第62回日本木材学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-16
• [Presentation] 高活性リグニン分解菌Phanerochaete sordida YK-624株によるビスフェノールAの分解 (2012)
  • Author(s): 王剣橋、河岸洋和、山本陽太郎、山本涼子、平井浩文
  • Organizer: 第62回日本木材学会大会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-03-16
• [Presentation] 高活性リグニン分解菌Phanerochaete sordida YK-624株による環境ホルモン類分解機構の解明 (2011)
  • Author(s): 山本陽太郎、平井浩文、河岸洋和
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学(京都市)
  • Year and Date: 2011-03-26
• [Presentation] 高活陸リグニン分解菌Phanerochaete sordida YK-624株による環境ホルモン類分解機構の解明 (2011)
  • Author(s): 山本陽太郎、平井浩文、河岸洋和
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学(京都府)
  • Year and Date: 2011-03-26
• [Presentation] 高活性リグニン分解菌Phanerochaete sordida YK-624株による環境ホルモン類分解機構の解明 (2010)
  • Author(s): 山本陽太郎、真島奈弓、平井浩文、河岸洋和
  • Organizer: 第60回日本木材学会大会
  • Place of Presentation: 宮崎市
  • Year and Date: 2010-03-19
• [Presentation] 高活性リグニン分解菌Phanerochaete sordida YK-624株にゆる環境ホルモン類分解機構の解明 (2010)
  • Author(s): 山本陽太郎、真島奈弓、平井浩文、河岸洋和
  • Organizer: 第60回日本木材学会大会
  • Place of Presentation: 宮崎観光ホテル
  • Year and Date: 2010-03-19
• [Remarks]
  • URL: http://www.agr.shizuoka.ac.jp/c/biochem/index.html
• [Remarks]
  • URL: http://www.agr.shizuoka.ac.jp/c/biochem/index.html