Identification of membrane-bound NAC transcription factor (ANAC078) target genes and investigation of its activation system
| Project/Area Number |
21780310
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tottori University |
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Principal Investigator |
YABUTA Yukinori Tottori University, 農学部, 准教授 (00379562)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 分子情報 / 転写因子 / 環境ストレス / ANAC078 / シロイヌナズナ |
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| Research Abstract |
To identify the genes targeted by ANAC078, we generated transgenic Arabidopsis plants overexpressing ANAC078 (Ox-ANAC078), and then conducted a DNA microarray analysis of Ox- ANAC078 and wild-type plants. In Ox-ANAC078 plants, the transcription of 166 genes was up-regulated compared with the levels in wild-type plants under high-light. The ANAC078-recognition sequence was detected in the promoter region of 52 up-regulated genes. These findings suggest that ANAC078 protein is associated with the induction of genes related to flavonoid biosynthesis and the regulation of proteasomes levels in response to HL stress. Furthermore, our findings suggest that the transcriptional activity of ANAC078 is regulated by cytoplasmic sequestration via transmembrane domain of C-terminal region of its protein.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Identification of recognition sequence of ANACO78 protein by the cyclic amplification and selection of targets technique.2010
Author(s)
Yabuta, Y., Morishita, T., Kojima, Y., Maruta, T., Nishizawa-Yokoi, A., Shigeoka, S.
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Journal Title
Plant Signal Behav. 5
Pages: 695-697
Related Report
Peer Reviewed
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[Journal Article] Arabidopsis NAC transcription factor, ANACO78, regulates flavonoids biosynthesis under high-light.2009
Author(s)
Morishita, T., Kojima, Y., Maruta, T., Nishizawa-Yokoi, A., Yabuta, Y., Shigeoka, S.
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Journal Title
Plant Cell Physiol. 50
Pages: 2210-2222
Related Report
Peer Reviewed
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[Presentation] Arabidopsis ANACO78 transcription factor regulates flavonoids biosynthesis under high-light.2010
Author(s)
Yabuta, Y., Morishita, T., Kojima, Y., Maruta, T., Nishizawa-Yokoi, A., Shigeoka, S.
Organizer
International conference on Arabidopsis Research
Place of Presentation
パシフィコ横浜
Year and Date
2010-06-08
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