Analysis of membrane protease function in fission yeast
| Project/Area Number |
21780311
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kagawa University |
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Principal Investigator |
TANAKA Naotaka Kagawa University, 農学部, 准教授 (60324109)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 分裂酵母 / ロンボイドプロテアーゼ / ゴルジ体 / タンパク質分解 |
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| Research Abstract |
In fission yeast Schizosaccharomyces pombe, two types of rhomboid proteases, named Rob1p and Rob2p, were localized at the Golgi apparatus. rob1Δ strain showed susceptibilities to zinc ion and cobalt ion, and missorting vacuole-type CPY (carboxi peptidase Y) to cells surface. Furthermore, there is a high probability that the Rob1p cleave membrane-bound transcripution factor at the golgi. rob2Δ strain was susceptibility to monensin which is a protein transport inhibitor between the Golgi and endosomes. These results indicate that the two types of rhomboid proteases are deeply-involved in protein sorting and activation of the Golgi resident proteins.
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Report
(3 results)
Research Products
(1 results)
