Elucidation of a mechanism of dehydration or heat stress signal transduction in plants through post-translational regulation of a transcription factor

• Project/Area Number: 21780314
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: The University of Tokyo
• Principal Investigator: MIZOI Junya 東京大学, 大学院・農学生命科学研究科, 特任助教 (20469753)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 発現制御 / 植物 / ストレス応答 / 転写因子 / 環境ストレス / 翻訳後調節 / モデル植物

Research Abstract

Plants increase tolerance to environmental stresses such as drought or heat in response to these stress stimuli. We analyzed the mechanism for the post-translational regulation of the transcription factor DREB2A in the stress response of Arabidopsis. We compared amino acid sequences among DREB2A orthologs in many plant species and analyzed the effects of site-directed mutagenesis on transcriptional activity of DREB2A. From these analyses, a core region that has a serine/threonine cluster was shown to be important for post-translational activation of the DREB2A protein.

Research Products

• [Journal Article] AP2/ERF family transcription factors in plant abiotic stress responses (2011)
  • Author(s): Mizoi, J., Shinozaki, K., Yamaguchi-Shinozaki, K
  • Journal Title: BBA-Gene Regul. Mech Volume: 1819 Issue: 2 Pages: 86-96
  • DOI: 10.1016/j.bbagrm.2011.08.004
  • Peer Reviewed
• [Journal Article] SPINDLY, a negative regulator of GA signaling, is involved in the plant abiotic stress response (2011)
  • Author(s): Qin, F., Kodaira, K., Maruyama, K., Mizoi, J., Tran, L.-S. P., Fujita, Y., Morimoto, K., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Journal Title: Plant Physiol Volume: 157 Issue: 4 Pages: 1900-1913
  • DOI: 10.1104/pp.111.187302
  • Peer Reviewed
• [Journal Article] An ABRE promoter sequence is involved in osmotic stress-responsive expression of the DREB2A gene, which encodes a transcription factor regulating drought-inducible genes in Arabidopsis (2011)
  • Author(s): Kim, J.-S., Mizoi, J., Yoshida, T., Fujita, Y., Nakajima, J., Ohori, T., Todaka, D., Nakashima, K., Hirayama, T., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Journal Title: Plant Cell Physiol Volume: 52 Issue: 12 Pages: 2136-2146
  • DOI: 10.1093/pcp/pcr143
  • NAID: 10030306452
  • Peer Reviewed
• [Journal Article] Comprehensive analysis of rice DREB2-type genes that encode transcription factors involved in the expression of abiotic stress-responsive genes (2010)
  • Author(s): Matsukura S, Mizoi J, Yoshida T, Todaka D, Ito Y, Maruyama K, Shinozaki K, Yamaguchi-Shinozaki K
  • Journal Title: Mol Genet Genomics Volume: 283 Issue: 2 Pages: 185-196
  • DOI: 10.1007/s00438-009-0506-y
  • Peer Reviewed
• [Journal Article] Comprehensive analysis of rice DREB2-type genes that encode transcription factors involved in the expression of abiotic stress-responsive genes (2010)
  • Author(s): Satoko Matsukura
  • Journal Title: Molecular Genetics and Genomics 283 Pages: 185-196
  • Peer Reviewed
• [Presentation] 緑色植物におけるAP2/ERF転写因子の分子系統解析 (2012)
  • Author(s): 溝井順哉
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-16
• [Presentation] 環境ストレス応答におけるシロイヌナズナのDREB2A (2012)
  • Author(s): 森本恭子
  • Organizer: タンパク質の安定性の解析
  • Place of Presentation: 京都
  • Year and Date: 2012-03-16
• [Presentation] ダイズの環境ストレス応答に関するDREB2A相同遺伝子の機能解析 (2012)
  • Author(s): 大堀鉄平
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-16
• [Presentation] 緑色植物におけるAP2/ERF転写因子の分子系統解析 (2012)
  • Author(s): 溝井順哉, 他
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都)
• [Presentation] 環境ストレス応答におけるシロイヌナズナのDREB2Aタンパク質の安定性の解析 (2012)
  • Author(s): 森本恭子, 他
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都)
• [Presentation] ダイズの環境ストレス応答に関するDREB2A相同遺伝子の機能解析 (2012)
  • Author(s): 大堀鉄平, 他
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都)
• [Presentation] Analysis of the relationship between stabilization and activation of the Arabidopsis transcription factor DREB2A under environmental stress (2011)
  • Author(s): Kyoko Morimoto
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Comparative functional analysis of DREB2A and DREB2B in Arabidopsis (2011)
  • Author(s): Junya Mizoi
  • Organizer: The 22nd International Conference on Arabidopsis Reseach
  • Place of Presentation: 米国・マディソン
  • Year and Date: 2011-06-23
• [Presentation] シロイヌナズナにおける相同な環境ストレス応答性転写因子DREB2AとDREB2Bの比較解析 (2011)
  • Author(s): 溝井順哉
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-21
• [Presentation] イネのDREB2型転写因子OsDREB2Bの機能解析 (2011)
  • Author(s): 松倉智子
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-21
• [Presentation] シロイヌナズナの転写因子DREB2Aの環境ストレスに応答した安定化と活性化に関する解析 (2011)
  • Author(s): 森本恭子
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-21
• [Presentation] ダイズの環境ストレス応答に関するDREB2A相同遺伝子の機能解析 (2011)
  • Author(s): 大堀鉄平
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-21
• [Presentation] シロイヌナズナにおける相同な環境ストレス応答性転写因子DREB2AとDREB2Bの比較解 (2011)
  • Author(s): 溝井順哉
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台(震災のため要旨のみ)
  • Year and Date: 2011-03-21
• [Presentation] イネのDREB2型転写因子OsDREB2Bの機能解析 (2011)
  • Author(s): 松倉智子
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台(震災のため要旨のみ)
  • Year and Date: 2011-03-21
• [Presentation] シロイヌナズナの転写因子DREB2Aの環境ストレスに応答した安定化と活性化に関する解析 (2011)
  • Author(s): 森本恭子
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台(震災のため要旨のみ)
  • Year and Date: 2011-03-21
• [Presentation] ダイズの環境ストレス応答に関するDREB2A相同遺伝子の機能解析 (2011)
  • Author(s): 大堀鉄平
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台(震災のため要旨のみ)
  • Year and Date: 2011-03-21
• [Presentation] Comparative functional analysis of DREB2A and DREB2B in Arabidopsis (2011)
  • Author(s): Mizoi, J., et al.
  • Organizer: 22nd International Conference on Arabidopsis Research
  • Place of Presentation: Wisconsin, USA
• [Presentation] Comparative functional analysis of two homologous transcription factors DREB2A and DREB2B in Arabidopsis (2011)
  • Author(s): Mizoi, J., et al.
  • Organizer: International Symposium "Strategies of Plants against Global Environmental Change"
  • Place of Presentation: 倉敷市芸文館(岡山)
• [Presentation] Analysis of the relationship between stabilization and activation of the Arabidopsis transcription factor DREB2A under environmental stress (2011)
  • Author(s): Morimoto, K., et al.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] Analysis of a stress-responsive stabilization mechanism of Arabidopsis DREB2A (2010)
  • Author(s): Junya Mizoi
  • Organizer: The 21st International Conference on Arabidopsis Research
  • Place of Presentation: 横浜
  • Year and Date: 2010-06-07
• [Presentation] Comprehensive analysis of rice DREB2-type genes that encode transcription factors involved in the expression of abiotic stress-responsive genes (2010)
  • Author(s): Satoko Matsukura
  • Organizer: The 21st International Conference on Arabidopsis Research
  • Place of Presentation: 横浜
  • Year and Date: 2010-06-07
• [Presentation] シロイヌナズナの転写因子DREB2Aのストレスに応答した安定化機構の解析 (2010)
  • Author(s): 溝井順哉
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-21
• [Presentation] イネの環境ストレス応答に関与するDREB2型転写因子の機能解析 (2010)
  • Author(s): 松倉智子
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-21
• [Presentation] シロイヌナズナの乾燥と高温ストレス誘導性遺伝子の発現制御機構におけるDREB2B遺伝子の機能解析 (2010)
  • Author(s): 安田奈保美
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-21
• [Presentation] SPINDLY, a negative regulator of GA signaling, involved in plant abiotic stress response (2010)
  • Author(s): Feng Qin
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-21
• [Presentation] シロイヌナズナの14-3-3タンパク質とAREB/ABF転写因子との相互作用の解明 (2010)
  • Author(s): 金俊植
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-21
• [Presentation] ダイズにおける環境ストレス応答性転写因子GmDREBの機能解析 (2010)
  • Author(s): 森脇崇
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-20
• [Presentation] シロイヌナズナの環境ストレス応答性転写因子DREB2Aの高温ストレス下における発現制御機構 (2009)
  • Author(s): 吉田拓実
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] A search for common regulatory elements of DREB2-type transcription factors based on a molecular phylogenetic analysis (2009)
  • Author(s): Junya Mizoi
  • Organizer: The 9th International Plant Molecular Biology Congress
  • Place of Presentation: 米国・セントルイス
  • Year and Date: 2009-10-28
• [Presentation] Transcriptional regulation of an Arabidopsis DREB2A gene under heat stress condition (2009)
  • Author(s): 吉田拓実
  • Organizer: The 9th International Plant Molecular Biology Congress
  • Place of Presentation: 米国・セントルイス
  • Year and Date: 2009-10-28
• [Book] 乾燥にも高温にも強い環境ストレス耐性植物の開発 (2009)
  • Author(s): 溝井順哉・篠崎和子
  • Publisher: シーエムシー出版