Functional analysis of small open reading frame in plants
Project/Area Number |
21780315
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Applied molecular and cellular biology
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Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
HIGUCHI Mieko 独立行政法人理化学研究所, 植物ゲノム機能研究グループ, 特別研究員 (40443014)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | short open reading frames / マイクロアレイ / シロイヌナズナ / 光誘導性遺伝子 / イネ / short open reading frame / 光誘導生 / 過剰発現体 |
Research Abstract |
To investigate function of sORFs in plants, I developed custom microarrays including sORFs and annotated genes in Arabidopsis to analyze gene expression profile. Microarray analysis was carried out in various tissue types. The expression of sORFs could be detected in various plant tissues. Full length cDNA of sORF was verified by RACE experiment. Next, promoter region and sORF without stop codon was fused to GUS gene and then expressed it in Arabidopsis. GUS signal was detected in transgenic plants, indicating that sORF was actually translated in plants. I selected sORFs based on expression, conservation, peptide hormone-like sequence to generate sORF overexpression Arabidopsis plants. Some overexpression plants showed visible phenotypes. Thus, I conclude that sORFs have roles in growth and developmental processes.
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Report
(4 results)
Research Products
(25 results)
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[Presentation] 環境ストレス耐性に関与するペプチドの探索2012
Author(s)
中南健太郎, 岡本昌憲, 樋口美栄子, 吉積毅, 清水みなみ, 大橋千広, 田中真帆, 松井南, 篠崎一雄, 関原明, 花田耕介
Organizer
日本植物生理学会
Place of Presentation
京都産業大学
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