Development of eastern blotting technique for high-throughput analysis of kampo medicines
| Project/Area Number |
21790030
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical pharmacy
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| Research Institution | Nagasaki International University |
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Principal Investigator |
MORINAGA Osamu Nagasaki International University, 薬学部, 講師 (60465771)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | イースタンブロット / モノクローナル抗体 / 漢方薬 / 配糖体 / 免疫染色 / イムノアッセイ / 品質評価 |
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| Research Abstract |
We demonstrated the high-throughput analysis for natural glycosides in kampo medicines using monoclonal antibodies (MAbs) and eastern blotting technique. Then, rapid, simple, high-sensitive and specific detection system was developed for GC and BI by eastern blotting technique using dot blot and chemiluminescent methods. This methods was useful as the high-throughput analysis for the determination of active compounds in kampo medicines as well as HPLC and ELISA systems.
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Report
(3 results)
Research Products
(19 results)
