Multifunctionalization of polymer surfaces for developing advanced cell culture substrate to support cell culture applications
| Project/Area Number |
21790038
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
SASAI Yasushi 岐阜薬科大学, 薬学部, 講師 (60336633)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ポリスチレン / 高分子表面機能化 / 表面界面物性 / 原子移動ラジカル重合 / プラズマ加工 / バイオインターフェイス / 生体分子固定化 / 細胞接着 / 細胞培養 / X線光電子分光法 / 細胞接着性ペプチド |
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| Research Abstract |
In order to prepare the functional substrate surfaces used in advanced cell culture applications, two methods were developed to introduce a large amount of carboxyl groups onto the chemically-inert polystyrene(PS) substrate. In one method, the biocompatible vinylmethylether-maleic acid copolymer(VEMAC) was immobilized on PS substrate through a coupling between carboxyl groups of VEMAC and hydroxyl groups on argon plasma-irradiated PS. In the other method, the well-defined poly(acrylic acid) brushes were fabricated on PS substrate by surface-initiated atom transfer radical polymerization of sodium acrylate. The surface carboxyl groups thus introduced were used for introducing a bioactive peptide interacting with cell surface. The immobilized peptide was specifically recognized by cell membrane receptors to stimulate cell adhesion and proliferation on the surface.
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Report
(4 results)
Research Products
(29 results)
