| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
In order to prepare the functional substrate surfaces used in advanced cell culture applications, two methods were developed to introduce a large amount of carboxyl groups onto the chemically-inert polystyrene(PS) substrate. In one method, the biocompatible vinylmethylether-maleic acid copolymer(VEMAC) was immobilized on PS substrate through a coupling between carboxyl groups of VEMAC and hydroxyl groups on argon plasma-irradiated PS. In the other method, the well-defined poly(acrylic acid) brushes were fabricated on PS substrate by surface-initiated atom transfer radical polymerization of sodium acrylate. The surface carboxyl groups thus introduced were used for introducing a bioactive peptide interacting with cell surface. The immobilized peptide was specifically recognized by cell membrane receptors to stimulate cell adhesion and proliferation on the surface.
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