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Multifunctionalization of polymer surfaces for developing advanced cell culture substrate to support cell culture applications

Research Project

Project/Area Number 21790038
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionGifu Pharmaceutical University

Principal Investigator

SASAI Yasushi  岐阜薬科大学, 薬学部, 講師 (60336633)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsポリスチレン / 高分子表面機能化 / 表面界面物性 / 原子移動ラジカル重合 / プラズマ加工 / バイオインターフェイス / 生体分子固定化 / 細胞接着 / 細胞培養 / X線光電子分光法 / 細胞接着性ペプチド
Research Abstract

In order to prepare the functional substrate surfaces used in advanced cell culture applications, two methods were developed to introduce a large amount of carboxyl groups onto the chemically-inert polystyrene(PS) substrate. In one method, the biocompatible vinylmethylether-maleic acid copolymer(VEMAC) was immobilized on PS substrate through a coupling between carboxyl groups of VEMAC and hydroxyl groups on argon plasma-irradiated PS. In the other method, the well-defined poly(acrylic acid) brushes were fabricated on PS substrate by surface-initiated atom transfer radical polymerization of sodium acrylate. The surface carboxyl groups thus introduced were used for introducing a bioactive peptide interacting with cell surface. The immobilized peptide was specifically recognized by cell membrane receptors to stimulate cell adhesion and proliferation on the surface.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (29 results)

All 2012 2011 2010 2009

All Journal Article (7 results) (of which Peer Reviewed: 6 results) Presentation (22 results)

  • [Journal Article] Fabrication of Scaffold for Cell Adhesion on Plasma-irradiated Polystyrene2011

    • Author(s)
      Yasushi Sasai, Yuko Tanaka, Shin-ichi Kondo, Yukinori Yamauchi, Masayuki Kuzuya
    • Journal Title

      Journal of Photopolymer Science and Technology

      Volume: 24 Issue: 4 Pages: 417-420

    • DOI

      10.2494/photopolymer.24.417

    • NAID

      130004464916

    • ISSN
      0914-9244, 1349-6336
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasma-based surface functionalizationfunctionalization of polystyrene substrate for cell culture application2011

    • Author(s)
      Yasushi Sasai, Yuko Tanaka, Shin-ichi Kondo, Yukinori Yamauchi, Masayuki Kuzuya
    • Journal Title

      Proc.

      Volume: 20 Pages: 454-454

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasma Surface Modification of Polymer Substrate for Cell Adhesion Control2010

    • Author(s)
      Yasushi Sasai, Shin-ichi Kondo, Yukinori Yamauchi, Masayuki Kuzuya
    • Journal Title

      Journal of Photopolymer Science and Technology

      Volume: 23 Issue: 4 Pages: 595-598

    • DOI

      10.2494/photopolymer.23.595

    • NAID

      130004833373

    • ISSN
      0914-9244, 1349-6336
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasma Surface Modification of Polymer Substrate for Cell Adhesion Control2010

    • Author(s)
      Yasushi Sasai, Shin-ichi Kondo, Yukinori Yamauchi, Masayuki Kuzuya
    • Journal Title

      Journal of photopolymer science and technology

      Volume: 23 Pages: 595-598

    • NAID

      130004833373

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Surface modification of polystyrene dishes using plasma techniques to enhance cell adhesion and proliferation2009

    • Author(s)
      Yasushi Sasai, Natsuko Matsuzaki, Shin-ichi Kondo, Yukinori Yamauchi, Masayuki Kuzuya
    • Journal Title

      Proc.

      Volume: 19

    • Related Report
      2011 Final Research Report
  • [Journal Article] Immobilization of Bioactive Molecule onto Polymer Surface functionalized by Plasma Techniques and its Application to Cell Culture2009

    • Author(s)
      Yasushi Sasai, Shin-ichi Kondo, Yukinori Yamauchi, Masayuki Kuzuya
    • Journal Title

      Journal of Photopolymer Science and Technology

      Volume: 22 Issue: 4 Pages: 503-506

    • DOI

      10.2494/photopolymer.22.503

    • NAID

      130004833291

    • ISSN
      0914-9244, 1349-6336
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immobilization of bioactive molecules onto polymer substrate functionalized by plasma techniques and its application to cell culture2009

    • Author(s)
      Yasushi Sasai, Shin-ichi Kondo, Yukinori Yamauchi, Masayuki Kuzuya
    • Journal Title

      Journal of photopolymer science and technology 22

      Pages: 503-506

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 選択的細胞接着表面の構築を目的とした原子移動ラジカル重合法によるポリスチレン基板の表面機能化2012

    • Author(s)
      笹井泰志
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Year and Date
      2012-03-30
    • Related Report
      2011 Final Research Report
  • [Presentation] 選択的細胞接着表面の構築を目的とした原子移動ラジカル重合法によるポリスチレン基板の表面機能化2012

    • Author(s)
      笹井泰志
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2012-03-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Surface modification of polystyrene substrate with well-defined poly(acrylic acid) brushes by atom transfer radical polymerization for cell culture application2012

    • Author(s)
      笹井泰志
    • Organizer
      12^<th> International Symposium on Biomimetic Materials Processing
    • Place of Presentation
      名古屋
    • Year and Date
      2012-01-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Surface modification of polystyrene substrate with well-defined poly(acrylic acid) brushes by atom transfer radical polymerization for cell culture application2012

    • Author(s)
      笹井泰志
    • Organizer
      11^<th> International symposium on biomimetic materials processing
    • Place of Presentation
      名古屋大学
    • Year and Date
      2012-01-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Plasma-based surface functionalization of polystyrene substrate for cell culture application2011

    • Author(s)
      笹井泰志
    • Organizer
      20th International Symposium on Plasma Chemistry
    • Place of Presentation
      フィラデルフィア(米国)
    • Year and Date
      2011-07-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Plasma-based surface functionalization of polystyrene substrate for cell culture application2011

    • Author(s)
      笹井泰志
    • Organizer
      20^<th> International conference on plasma chemistry
    • Place of Presentation
      フィラデルフィア(米国)
    • Year and Date
      2011-07-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] プラズマ照射ポリスチレン表面を利用する細胞接着の足場構築2011

    • Author(s)
      笹井泰志
    • Organizer
      第28回国際フォトポリマーコンファレンス
    • Place of Presentation
      千葉
    • Year and Date
      2011-06-23
    • Related Report
      2011 Final Research Report
  • [Presentation] プラズマ照射ポリスチレン表面を利用する細胞接着の足場構築2011

    • Author(s)
      笹井泰志
    • Organizer
      第28回国際フォトポリマーコンファレンス
    • Place of Presentation
      千葉大学
    • Year and Date
      2011-06-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Fabrication of extracellular matrix-like peptide conjugate on plasma-irradiated polystyrene dish2011

    • Author(s)
      笹井泰志
    • Organizer
      11^<th> International Symposium on Biomimetic Materials Processing
    • Place of Presentation
      名古屋
    • Year and Date
      2011-01-25
    • Related Report
      2011 Final Research Report
  • [Presentation] Fabrication of extracellular matrix-like peptide conjugate on plasma-irradiated polystyrene dish2011

    • Author(s)
      笹井泰志
    • Organizer
      11^<th> International symposium on biomimetic materials processing
    • Place of Presentation
      名古屋大学
    • Year and Date
      2011-01-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Fabrication of artificial extracellular matrix on plasmafunctionalized polystyrene substrate to control cell behavior2010

    • Author(s)
      笹井泰志
    • Organizer
      10^<th> Asia-Pacific 48時間後GRGDS固定化領域GRGDS非固定化領域Conference on Plasma Science and Technology & 23rd Symposium on Plasma Science for Materials
    • Place of Presentation
      済州島(韓国)
    • Year and Date
      2010-07-07
    • Related Report
      2011 Final Research Report
  • [Presentation] Fabrication of artificial extracellular matrix on plasma-functionalized polystyrene substrate to control cell behavior2010

    • Author(s)
      笹井泰志
    • Organizer
      10^<th> Asia-pacific conference on plasma science and technology & 23^<rd> Symposium on plasma science for materials)
    • Place of Presentation
      済州島(韓国)
    • Year and Date
      2010-07-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細胞接着制御を目的としたプラズマ技術を利用する高分子表面修飾2010

    • Author(s)
      笹井泰志
    • Organizer
      第27回国際フォトポリマーコンファレンス
    • Place of Presentation
      千葉
    • Year and Date
      2010-06-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞接着制御を目的としたプラズマ技術を利用する高分子表面修飾2010

    • Author(s)
      笹井泰志
    • Organizer
      第27回 国際フォトポリマーコンファレンス
    • Place of Presentation
      千葉大学
    • Year and Date
      2010-06-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] プラズマ技術を利用したポリスチレン基板の表面機能化による細胞接着制御2010

    • Author(s)
      笹井泰志
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] プラズマ技術を利用したポリスチレン基板の表面機能化による細胞接着制御2010

    • Author(s)
      笹井泰志
    • Organizer
      日本薬学会第130年会(岡山)
    • Place of Presentation
      岡山大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Plasma-assisted functionalization of polystyrene surface to enhance cell adhesion and proliferation2010

    • Author(s)
      笹井泰志
    • Organizer
      10^<th> International Symposium on Biomimetic Materials Processing
    • Place of Presentation
      名古屋
    • Year and Date
      2010-01-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Plasma-assistedfhnctionalization of polystyrene surface to enhance cell adhesion and prolifbration2010

    • Author(s)
      笹井泰志
    • Organizer
      10th Intemational symposium on biomimetic materials processing
    • Place of Presentation
      名古屋大学
    • Year and Date
      2010-01-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] Surface modification of polystyrene dishes using plasma techniques to enhance cell adhesion and proliferation2009

    • Author(s)
      笹井泰志
    • Organizer
      19th International Symposium on Plasma Chemistry
    • Place of Presentation
      ボーフム(ドイツ)
    • Year and Date
      2009-07-30
    • Related Report
      2011 Final Research Report
  • [Presentation] Surface modification of polystyrene dishes using plasma techniques to enhance cell adhesion and proliferation2009

    • Author(s)
      笹井泰志
    • Organizer
      19THINTERNATIONAL SYMPOSIUMONPLASMA CHEMISTRY
    • Place of Presentation
      ボーフム(ドイツ)
    • Year and Date
      2009-07-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] プラズマ技術により機能化した高分子表面への生理活性分子の固定化とその細胞培養への応用2009

    • Author(s)
      笹井泰志
    • Organizer
      第26回国際フォトポリマーコンファレンス
    • Place of Presentation
      千葉
    • Year and Date
      2009-07-02
    • Related Report
      2011 Final Research Report
  • [Presentation] プラズマ技術により機能化した高分子表面への生理活性分子の固定化とその細胞培養への応用2009

    • Author(s)
      笹井泰志
    • Organizer
      第26回国際フォトポリマーコンファレンス
    • Place of Presentation
      千葉大学
    • Year and Date
      2009-07-02
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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