Development of ultrasensitive bioluminescent immunoassay by using DNA amplification technique
| Project/Area Number |
21790042
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Tohoku Pharmaceutical University (2011)
Showa University (2009-2010) |
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Principal Investigator |
OHNO Ken-ichi 東北薬科大学, 薬学部, 講師 (20347272)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | イムノアッセイ / 遺伝子増幅法 / 生物発光検出 / 臨床検査 / 診断 / スクリーニング / 検査 |
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| Research Abstract |
In this study, a highly sensitive immunoassay was developed by using DNA amplification technique as detection system. Under isothermal condition, LAMP(Loop-mediated isothermal Amplification) and RCA(Rolling Circle Amplification) reaction enable successive DNA amplification. In order to apply DNA amplification to the detection system on immunoassay, DNA-conjugated antigen was prepared for competitive immunoassay. As a result, physically and chemically stable DNA provided highly efficient and sensitive signal amplification based on genetic engineering.
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Report
(4 results)
Research Products
(5 results)
