Lysophosphatidic acid regulates hair follicle development

• Project/Area Number: 21790058
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Tohoku University
• Principal Investigator: INOUE Asuka 東北大学, 大学院・薬学研究科, 助手 (50525813)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: リゾリン脂質 / 毛包 / 受容体 / GPCR / リゾホスファチジン酸 / 産生酵素 / 毛根 / TGFα / 体毛形成 / LPA

Research Abstract

Lysophosphatidic acid has been established as an important mediator that is involved in various physiological events. Our work is to examine novel roles of lysophosphatidic acid by utilizing genetically engineered mice. Here we found that mice lacking production of lysophophatidic acid exhibited hair defects. We further identified mechanisms underlying the lysophophatidic acid-induced hair formation. Our work will be beneficial to development of medicine for hair disorders.

Research Products

• [Journal Article] Crystal structure of autotaxin and insight into GPCR activation by lipid mediators (2011)
  • Author(s): Nishimasu H, Okudaira S, Hama K, Mihara E, Dohmae N, Inoue A, Ishitani R, Takagi J, Aoki J, Nureki O.
  • Journal Title: Nat Struct Mol Biol. Volume: 18(2) Pages: 205-212
  • Peer Reviewed
• [Journal Article] Crystal structure of autotaxin and insight into GPCR activation by lipid mediators (2011)
  • Author(s): Nishimasu H, et al
  • Journal Title: Nat Struct Mol Biol Volume: 18 Pages: 205-212
  • Peer Reviewed
• [Journal Article] Prevalent LIPH founder mutations lead to loss of P2Y5 activation ability of PA-PLAlalpha in autosomal recessive hypotrichosis (2010)
  • Author(s): Shinkuma S, Akiyama M, Inoue A, Aoki J, Natsuga K, Nomura T, Arita K, Abe R, Ito K, Nakamura H, Ujiie H, Shibaki A, Suga H, Tsunemi Y, Nishie W, Shimizu H.
  • Journal Title: Hum Mutat. Volume: 31(5) Pages: 602-610
  • Peer Reviewed
• [Journal Article] Prevalent LIPH founder mutations lead to loss of P2Y5 activation ability of PA-PLA1alpha in autosomal recessive hypotrichosis (2010)
  • Author(s): Shinkuma S, et al
  • Journal Title: Hum Mutat Volume: 31 Pages: 602-610
  • Peer Reviewed
• [Journal Article] 第二世代の生理活性脂質としてのリゾリン脂質 (2009)
  • Author(s): 井上飛鳥、青木淳賢
  • Journal Title: 実験医学 Volume: 27(13) Pages: 2059-2067
• [Journal Article] 第二世代の生理活性脂質としてのリゾリン脂質 (2009)
  • Author(s): 井上飛鳥、青木淳賢
  • Journal Title: 実験医学 27 Pages: 2059-2067
• [Presentation] LC-ESI-MS/MSを用いた生理活性リゾリン脂質定量系の開発 (2011)
  • Author(s): 井上飛鳥
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-28
• [Presentation] 炎症時におけるリゾホスファチジルセリン産生の変動解析 (2011)
  • Author(s): 奥谷倫世
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡、静岡県コンベンションアーツセンター
  • Year and Date: 2011-03-28
• [Presentation] 新規GPCRシグナル検出法-TGFα切断アッセイ-の開発 (2011)
  • Author(s): 石黒純
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡、静岡県コンベンションアーツセンター
  • Year and Date: 2011-03-28
• [Presentation] LC-ESI-MS/MSを用いた生理活性リゾリン脂質定量系の開発 (2011)
  • Author(s): 井上飛鳥
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡、静岡県コンベンションアーツセンター
  • Year and Date: 2011-03-28
• [Presentation] LC-ESI-MS/MSを用いたリゾリン脂質定量系の開発 (2010)
  • Author(s): 井上飛鳥
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] LC-ESI-MS/MSを用いたリゾホスファチジルセリンおよびリゾホスファチジルスレオニンの検出 (2010)
  • Author(s): 奥谷倫世
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫、神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] 胎生期骨形成におけるLPAシグナリングの役割の解明 (2010)
  • Author(s): 板井恵理子
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫、神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] 広範なGPCRシグナルを検出する新規測定法-TGFα切断アッセイ-の開発 (2010)
  • Author(s): 石黒純
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫、神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] リゾホスファチジルセリンの活性化リンパ球に対する効果 (2010)
  • Author(s): 北村一
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫、神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] PLA1型リゾリン脂質産生酵素の受容体活性化機構の解析 (2010)
  • Author(s): 首藤啓明
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫、神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] LC-ESI-MS/MSを用いたリゾホスファチジルセリンの検出 (2010)
  • Author(s): 奥谷倫世
  • Organizer: 第49回日本薬学会東北支部大会
  • Place of Presentation: 福島、奥羽大学
  • Year and Date: 2010-10-24
• [Presentation] TGFα切断アッセイを用いたオーファンGPCRのリガンドスクリーニング (2010)
  • Author(s): 石黒純
  • Organizer: 第9回次世代を担う若手ファーマ・バイオフォーラム2010
  • Place of Presentation: 京都、京都大学大学院薬学研究科
  • Year and Date: 2010-10-02
• [Presentation] LPS受容体P2Y10の同定と細胞接着における機能 (2010)
  • Author(s): 北村一
  • Organizer: 第9回次世代を担う若手ファーマ・バイオフォーラム2010
  • Place of Presentation: 京都、京都大学大学院薬学研究科
  • Year and Date: 2010-10-02
• [Presentation] Lysophosphatidic acid plays a critical role in hair follicle formation through TGF-alpha release and EGFR transactivation (2010)
  • Author(s): Asuka Inoue
  • Organizer: he 27th Naito Conference on Membrane Dynamics and Lipid Biology [I]
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2010-06-29
• [Presentation] Lysophosphatidic acid plays a critical role in hair follicle formation through TGF-alpha release and EGFR transactivation (2010)
  • Author(s): 井上飛鳥
  • Organizer: The 27th Naito Conference on Menmbrane Dynamics and Lipid Biology [I]
  • Place of Presentation: Sapporo, CHATERAISE Gateaux Kingdom SAPPORO
  • Year and Date: 2010-06-29
• [Presentation] LC-ESI-MS/MSを用いたリゾリン脂質定量系の開発 (2010)
  • Author(s): 井上飛鳥
  • Organizer: 第52回日本脂質生化学会
  • Place of Presentation: 群馬
  • Year and Date: 2010-06-15
• [Presentation] LC-ESI-MS/MSを用いたリゾリン脂質定量系の開発 (2010)
  • Author(s): 井上飛鳥
  • Organizer: 第52回日本脂質生化学会
  • Place of Presentation: 群馬、秋森旅館
  • Year and Date: 2010-06-15
• [Presentation] TGPα切断アッセイによるGPCRシグナルの検出 (2010)
  • Author(s): 石黒純
  • Organizer: 第62回日本脂質生化学会
  • Place of Presentation: 群馬、秋森旅館
  • Year and Date: 2010-06-15
• [Presentation] オートタキシントランスジェニックマウスの解析 (2010)
  • Author(s): 雪浦弘志
  • Organizer: 第52回日本脂質生化学会
  • Place of Presentation: 群馬、秋森旅館
  • Year and Date: 2010-06-15
• [Presentation] Lysophosphatidic acid plays a critical role in hair follicle formation through TGFa release and EGFR transactivation (2010)
  • Author(s): Asuka Inoue
  • Organizer: Keystone Symposia Bioactive Lipids: Biochemistry and Diseases (D2)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-06-07
• [Presentation] Lysophosphatidic acid plays a critical role in hair follicle formation through TGFa release and EGFR transactivation (2010)
  • Author(s): 井上飛鳥
  • Organizer: Keystone Symposia Bioactive Lipids : Biochemistry and Diseases (D2)
  • Place of Presentation: Kyoto, Westin Miyako Kyoto
  • Year and Date: 2010-06-07
• [Presentation] TGFα切断アッセイによるG12/13シグナルの検出 (2010)
  • Author(s): 石黒純
  • Organizer: 第7回GPCR研究会
  • Place of Presentation: 東京、日本科学未来館みらいCANホール
  • Year and Date: 2010-05-07
• [Presentation] Cleavage of membrane-bound TGFa as a useful readout for GPCR activation (2010)
  • Author(s): Asuka Inoue
  • Organizer: Satellite Symposium of the 14th International Congress of Endocrinology (ICE2010)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-03-30
• [Presentation] Cleavage of membrane-bound TGFa as a useful readout for GPCR activation (2010)
  • Author(s): Asuka Inoue, Jun Ishiguro, Naoaki Arima, Shigeki Higashiyama, Junken Aoki
  • Organizer: Satellite Symposium of the 14th International Congress of Endocrinology(ICE2010)
  • Place of Presentation: 京都
  • Year and Date: 2010-03-30
• [Presentation] 広範囲のGPCRシグナル検出を目的としたGαタンパク質共発現によるTGFα切断アッセイ系の確立 (2009)
  • Author(s): 井上飛鳥
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] Lysophosphatidic acid plays a critical roles in hair follicle formation via EGFR transactivation (2009)
  • Author(s): 井上飛鳥、有馬直明、新井洋由、青木淳賢
  • Organizer: 第十回 文部科学省特定領域研究「がん」5領域 若手研究者ワークショップ
  • Place of Presentation: 蓼科
  • Year and Date: 2009-09-03
• [Presentation] Lysophosphatidic acid plays a critical role in hair follicle formation through TGFa cleavage (2009)
  • Author(s): Asuka Inoue
  • Organizer: FASEB Summer Research Conference "Lysophospholipid Mediators in Health and Disease"
  • Place of Presentation: Arizona, USA
  • Year and Date: 2009-06-30
• [Presentation] Lysophosphatidic acid plays a critical role in hair follicle formation through TGFa cleavage (2009)
  • Author(s): Asuka Inoue, Naoaki Arima, Hiroyuki Arai, Junken Aoki
  • Organizer: FASEB Summer Research Conference "Lysophospholipid Mediators in Health and Disease"
  • Place of Presentation: アメリカ合衆国、アリゾナ州ケアフリー
  • Year and Date: 2009-06-30
• [Presentation] LYSOPHOSPHATIDIC ACID PLAYS A CRITICAL ROLE IN HAIR FOLLICLE FORMATION THROUGH TGF-ALPHA SHEDDING (2009)
  • Author(s): Asuka Inoue
  • Organizer: 4th International Conference on Phospholipase A2 and Lipid Mediators (PLM2009)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2009-05-27
• [Presentation] LYSOPHOSPHATIDIC ACID PLAYS A CRITICAL ROLE IN HAIR FOLLICLE FORMATION THROUGH TGF-ALPHA SHEDDING (2009)
  • Author(s): Asuka Inoue, Naoaki Arima, Hiroyuki Arai, Junken Aoki
  • Organizer: 4th International Conference on Phospholipase A2 and Lipid Mediators(PLM2009)
  • Place of Presentation: 東京
  • Year and Date: 2009-05-27
• [Presentation] Lysophosphatidic acid is involved in hair follicle formation (2009)
  • Author(s): Asuka Inoue
  • Organizer: he 5th Takeda Science Foundation Symposium on PharmaSciences
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2009-05-25
• [Presentation] Lysophosphatidic acid is involved in hair follicle formation (2009)
  • Author(s): Asuka Inoue, Naoaki Arima, Hiroyuki Arai, Junken Aoki
  • Organizer: The 5th Takeda Science Foundation Symposium on PharmaSciences
  • Place of Presentation: 東京
  • Year and Date: 2009-05-25
• [Presentation] EGF受容体リガンド切断アッセイを用いた新規リゾホスファチジン酸受容体P2Y5の機能解析 (2009)
  • Author(s): 井上飛鳥
  • Organizer: 第6回GPCR研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-05-08
• [Remarks]
  • URL: http://www.pharm.tohoku.ac.jp/seika/H2l/index.html
• [Remarks]
  • URL: http://www.pharm.tohoku.ac.jp/~seika/H21/index.html
• [Remarks]
  • URL: http://www.pharm.tohoku.ac.jp/%7Eseika/H21/index.html