| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
In this study, we obtained following results:
(1) Using immunoprecipitation coupled with mass spectrometry, several PAFAH2-interacting proteins including PRDX2 and SCP2 were identified.
(2) From mutational analysis of PAFAH2, Cys-83 was found to be the only residue conferring sensitivity of PAFAH2 to DTNB, suggesting that PAFAH2 activity can be regulated by modification of Cys-83.
(3) An immunocytochemical assay to detect the translocation of PAFAH2 from cytosol to membrane was established.
(4) PAFAH2-deficient mice exhibited reduced PCA reaction most probably by impaired mast cell degranulation.
(5) Bone marrow-derived mast cells from PAFAH2-deficient mice also showed decreased IgE-dependent degranulation.
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